A universal fluorescence biosensor based on rolling circle amplification and locking probe for DNA detection

Fang, Ying; Nie, Lanxin; Wang, Suqin; Liu, Shiwen; Li, Hongbo; Yu, Ruqin

doi:10.1007/s00604-024-06501-2

Microchim Acta

2024

DOI: 10.1007/s00604-024-06501-2

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A universal fluorescence biosensor based on rolling circle amplification and locking probe for DNA detection

Ying Fang,

Lanxin Nie,

Suqin Wang

et al.

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Cited by 2 publications

References 42 publications

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Rolling circle amplification cooperating crRNA switch for direct and sensitive methicillin-resistant Staphylococcus aureus (MRSA) analysis

Qiu,

Liu,

Zhu

2024

Biotechnol Lett

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Rolling circle amplification cooperating crRNA switch for direct and sensitive methicillin-resistant Staphylococcus aureus (MRSA) analysis

Qiu,

Liu,

Zhu

2024

Biotechnol Lett

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Single Nucleotide Recognition and Mutation Site Sequencing Based on a Barcode Assay and Rolling Circle Amplification

Zhong,

Chen,

Cao

et al. 2024

Biosensors

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Single nucleotide polymorphisms (SNPs) present significant challenges in microbial detection and treatment, further raising the demands on sequencing technologies. In response to these challenges, we have developed a novel barcode-based approach for highly sensitive single nucleotide recognition. This method leverages a dual-head folded complementary template probe in conjunction with DNA ligase to specifically identify the target base. Upon recognition, the system triggers rolling circle amplification (RCA) followed by the self-assembly of CdSe quantum dots onto polystyrene microspheres, enabling a single-particle fluorescence readout. This approach allows for precise base identification at individual loci, which are then analyzed using a bio-barcode array to screen for base changes across multiple sites. This method was applied to sequence a drug-resistant mutation site in Helicobacter pylori (H. pylori), demonstrating excellent accuracy and stability. Offering high precision, high sensitivity, and single nucleotide resolution, this approach shows great promise as a next-generation sequencing method.

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A universal fluorescence biosensor based on rolling circle amplification and locking probe for DNA detection

Cited by 2 publications

References 42 publications

Rolling circle amplification cooperating crRNA switch for direct and sensitive methicillin-resistant Staphylococcus aureus (MRSA) analysis

Rolling circle amplification cooperating crRNA switch for direct and sensitive methicillin-resistant Staphylococcus aureus (MRSA) analysis

Single Nucleotide Recognition and Mutation Site Sequencing Based on a Barcode Assay and Rolling Circle Amplification

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