A Universal LC-MS/MS Method for Simultaneous Detection of Antibiotic Residues in Animal and Environmental Samples

Chan, Chak-Lun; Wai, Hogan Kok-Fung; Wu, Peng; Lai, Siu‐Wai; Chan, Olivia Sinn-Kay; Tun, Hein Min

doi:10.3390/antibiotics11070845

Cited by 18 publications

(8 citation statements)

References 18 publications

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“…Other first-line and critically important antimicrobials in human use described by the World Health Organization, antibiotics such as amoxicillin (14%), sulphonamides and sulfa-trimethoprim combinations (15%), neomycin (10%), oxytetracyline (9%), and enrofloxacin (6%), were commonly used in poultry. Expanding on publications regarding laboratory and epidemiological synergy [ 24 ], it is important to consider further AMU study by triangulating the presence of antibiotics in animal muscle and the farming environment by laboratory means, together with sales, and AMU survey data [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

One Health Surveillance of Antimicrobial Use and Resistance: Challenges and Successes of Implementing Surveillance Programs in Sri Lanka

Ariyawansa

Gunawardana

Hapudeniya³

et al. 2023

Antibiotics

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Background: Sri Lanka is a low-income country, as defined by the World Bank. The country suffered further economic downturn during the COVID-19 pandemic. This situation adversely affected the prioritization of policies and programs around healthcare and public health. In particular, inflation, fuel prices, and shortage of food supplies increased struggles to implement antimicrobial resistance (AMR) programs. However, in the long run, it is crucial to gather data and evidence to plan AMR policies and track interventions. (1) Aim: To establish and reiterate the importance of prioritizing AMR programs in the One Health framework, the Fleming Fellows collected and studied antimicrobial use/consumption (AMU/AMC) and resistance (AMR) in humans, food-producing animals, and the environment. (2) Methods: A systematic and cross-sectional study was conducted between 2019 and 2021. By way of coordinating an AMU/AMC and AMR prevalence study across six agencies from human health and food-producing animal sectors, the authors established a field epidemiology study, laboratory testing, and data processing at their institutions. AMU/AMC patterns were surveyed using questionnaires and interviews, while AMR samples were collected for antibiotic susceptibility tests and genomic tests. Samples were tested for phenotypic and genotypic resistance. (3) Results: In human samples, resistance was highest to beta-lactam antibiotics. In non-human samples, resistance was highest to erythromycin, a highest-priority, critically important antibiotic defined by the World Health Organization. From government records, tylosin was sold the most in the food-producing animal sector. (4) Conclusions: Sri Lanka AMU and AMR trends in human and non-human sectors can be ascertained by a One Health framework. Further coordinated, consistent, and sustainable planning is feasible, and can help implement an AMU/AMR surveillance system in Sri Lanka.

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Section: Discussionmentioning

confidence: 99%

One Health Surveillance of Antimicrobial Use and Resistance: Challenges and Successes of Implementing Surveillance Programs in Sri Lanka

Ariyawansa

Gunawardana

Hapudeniya³

et al. 2023

Antibiotics

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“…Certain approaches that have been described for quantifying residual antibiotics in influent, effluent, groundwater, and surface water are assumed to use costly liquid–liquid extraction sample extraction techniques. Few liquid chromatography-tandem mass spectrometry (LC–MS/MS) techniques were reported for the analysis of pharmaceutical substances in effluent, influent, surface, groundwater, and wastewater treatment plant using solid phase extraction (SPE), − electrospray ionization source, , and MS/MS . ultra-performance LC (UPLC)-MS/MS methods have also been reported. , …”

Section: Introductionmentioning

confidence: 99%

Development and Validation of UFLC-MS/MS Analytical Method for the Simultaneous Quantification of Antibiotic Residues in Surface Water, Groundwater, and Pharmaceutical Waste Water Samples from South India

Kamatham,

Seeralan,

Sekar

et al. 2024

ACS Omega

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Antibiotic residues in pharmaceutical wastewater pose a significant environmental concern due to their potential role in fostering antimicrobial resistance. South Indian pharmaceutical companies produce a wide range of antibiotics. As a result, the industries that discharge water may include antibiotic residues, which could be harmful to the environment. In this study, a novel, quick, accurate, and sensitive approach for the simultaneous detection of 11 antibiotics was established, and triple quadrupole mass spectrometry, ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS), and selective solid-phase extraction (SPE) were used for validation. Utilizing a mixed mode reversed-phase/cationexchange cartridge (SPE using Strata X, 33 μm), the single-cartridge extraction procedure was performed and validated. Relative standard deviations for most of the antibiotics ranged from 3.5 to 0.56 with recoveries ranging from 57 to 85%. The samples were injected into the UFLC-MS/ MS apparatus at a volume of 10 μL for analysis. The auto sampler cooler temperature was kept at 150 °C, while the column temperature was kept at 40 °C. After validation, the technique was determined to be linear in the range of 2.0−1000.0 ng/mL. The retention period for antibiotics was between 1.2 and 1.5 min. Antibiotics transitions for multiple reaction monitoring| were between 235.1/105.9 and 711.5/467.9 m/z. The method of analysis took 2.5 min to run completely. Antibiotic residues were efficiently analyzed using the established analytical approach in pharmaceutical wastewater (influent and effluent), surface, and groundwater. Eleven antibiotics were found in the water samples during examination with concentrations ranging between 2.313 and 95.744 ng/L. The procedure was shown to be much more environmentally friendly than other contemporary methods based on the green analytical procedure index's evaluation of greenness. Blue applicability grade index tool indicated the developed method's practicality in comparison with that of other reported method.

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“…Several analytical techniques were developed in recent years for the detection of STR in environmental and food samples including chromatographic methods such as high-performance liquid chromatography (HPLC), 11 liquid chromatography-mass spectrometry (LC-MS), 12 gas chromatography-mass spectrometry (GC-MS), 13 and LC-MS/MS 14 and immunoassay method such as enzyme-linked immunosorbent assay (ELISA). 15 Among these methods, ELISA is a quick, robust and high-throughput alternative to other methods used in trace analysis but it has serious limitations such as instability of the antibodies used, high cost, and low shell-life of the kits.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of molecularly imprinted cotton fibers for quantification of streptomycin in honey samples

Zengi̇n

Badak

Bilici

et al. 2023

J of Applied Polymer Sci

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Streptomycin is a type of aminoglycoside used in treatment of several infections and with serious side effects in humans and animals, such as ototoxicity and nephrotoxicity. In the present study, we focused on the preparation of streptomycin‐imprinted cotton fiber for rapid, sensitive and selective quantification of streptomycin in honey samples. The imprinted cotton fibers were characterized by attenuated total reflectance‐Fourier transform spectroscopy, scanning electron microscopy, and x‐ray photoelectron spectroscopy. In addition, the imprinted cotton fibers showed high adsorption capacity (95.4 mg/g) with an imprinting factor of 3.69 and had satisfactory regeneration ability of up to 10 regenerations without significant change in adsorption capacity. The imprinted cotton fibers showed good linearity with a correlation coefficient of 0.9989 and limit of detection was determined to be 0.29 ng/mL for streptomycin in honey samples. The proposed method showed acceptable recovery ranges (98.0%–100.3%) with lower inter‐day and intra‐day precisions. Moreover, the performance of the proposed method was compared to the ELISA method. The results showed that the present method is as sensitive and selective as ELISA. It is concluded that the imprinted cotton fibers could be a potential alternative to antibiotic quantification based on traditional immunoassays or chromatographic techniques.

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A Universal LC-MS/MS Method for Simultaneous Detection of Antibiotic Residues in Animal and Environmental Samples

Cited by 18 publications

References 18 publications

One Health Surveillance of Antimicrobial Use and Resistance: Challenges and Successes of Implementing Surveillance Programs in Sri Lanka

One Health Surveillance of Antimicrobial Use and Resistance: Challenges and Successes of Implementing Surveillance Programs in Sri Lanka

Development and Validation of UFLC-MS/MS Analytical Method for the Simultaneous Quantification of Antibiotic Residues in Surface Water, Groundwater, and Pharmaceutical Waste Water Samples from South India

Fabrication of molecularly imprinted cotton fibers for quantification of streptomycin in honey samples

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