A Universal Stress Protein from Medicago falcata (MfUSP1) confers multiple stress tolerance by regulating antioxidant defense and proline accumulation

Gou, Lanming; Zhuo, Chunliu; Lu, Shengyong; Guo, Zhenfei

doi:10.1016/j.envexpbot.2020.104168

Cited by 24 publications

(18 citation statements)

References 44 publications

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“…Plants under stressful environments accumulate several compatible molecules like GB, proline and sugars to overcome osmotic stress triggered by several abiotic stress conditions 73 , 74 . Excessive proline amassing under stress helps plants to maintain redox potential and help in osmotic adjustments for stabilizing cellular structures and ROS scavenging 75 , 76 .…”

Section: Discussionmentioning

confidence: 99%

Jasmonic acid (JA) and gibberellic acid (GA3) mitigated Cd-toxicity in chickpea plants through restricted cd uptake and oxidative stress management

Ahmad

Raja²,

Ashraf

et al. 2021

Sci Rep

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Cadmium stress is one of the chief environmental cues that can substantially reduce plant growth. In the present research, we studied the effect of jasmonic acid (JA) and gibberellic acid (GA3) applied individually and/or in combination to chickpea (Cicer arietinum) plants exposed to 150 µM cadmium sulphate. Cadmium stress resulted in reduced plant growth and pigment contents. Moreover, chickpea plants under cadmium contamination displayed higher levels of electrolytic leakage, H2O2, and malonaldehyde, as well as lower relative water content. Plants primed with JA (1 nM) and those foliar-fed with GA3 (10–6 M) showed improved metal tolerance by reducing the accumulation of reactive oxygen species, malonaldehyde and electrolytic leakage, and increasing relative water content. . Osmoprotectants like proline and glycinebetaine increased under cadmium contamination. Additionally, the enzymatic activities and non-enzymatic antioxidant levels increased markedly under Cd stress, but application of JA as well as of GA3 further improved these attributes. Enzymes pertaining to the ascorbate glutathione and glyoxylase systems increased significantly when the chickpea plants were exposed to Cd. However, JA and GA3 applied singly or in combination showed improved enzymatic activities as well as nutrient uptake, whereas they reduced the metal accumulation in chickpea plants. Taken together, our findings demonstrated that JA and GA3 are suitable agents for regulating Cd stress resistance in chickpea plants.

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Section: Discussionmentioning

confidence: 99%

Jasmonic acid (JA) and gibberellic acid (GA3) mitigated Cd-toxicity in chickpea plants through restricted cd uptake and oxidative stress management

Ahmad

Raja²,

Ashraf

et al. 2021

Sci Rep

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“…In contrast, while a gene encoding an adenine nucleotide alpha hydrolase-like superfamily protein (Medtr1g054765), which was previously termed MfUSP1 in M. sativa subsp. falcata [ 19 ] and falls within the ethylene-induced-regulated-responsive-activated bin in MapMan, was only up-regulated under drought conditions in ‘sativa’ (log2 fold-change of 1.57089; File S2 ), this gene was significantly up-regulated in ‘falcata’ compared to ‘sativa’ under both well-watered (3.43631 log2 fold-change) and drought (2.56688 log2 fold-change) conditions ( Figure S12b, File S2 ).…”

Section: Resultsmentioning

confidence: 99%

“…Until fairly recently, the enhancement of drought resilience has not been a major focus of alfalfa breeding efforts, and the scope of genetic variation and mechanisms responsible for this trait are not fully known [ 14 ]. While conventional breeding programs have begun to focus on the improvement of this trait in alfalfa [ 2 , 15 ], and a growing number of studies are dedicated towards the enhancement of drought tolerance in alfalfa using a transgenic approach [ 16 , 17 , 18 , 19 , 20 ], such efforts are complicated by a lack of understanding of the exact mechanisms by which alfalfa senses, reacts, and adapts to water deficit. Medicago sativa subsp.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, various studies have demonstrated that the heterologous expression of certain genes from M. sativa subsp. falcata genotypes, including those encoding Universal Stress Protein 1 ( MfUSP1 ) [ 19 ], galactinol synthase ( MfGolS1 ) [ 16 ], myo -inositol phosphate synthase ( MfMIPS1 ) [ 28 ], and late embryogenesis abundant protein 3 ( MfLEA3 ) [ 20 ], in tobacco led to enhanced tolerance to osmotic or dehydration stress in transgenic lines. Although comparative transcriptional responses between a drought-sensitive sativa (Chilean) and a drought-tolerant falcata (Wisfal) variety have been assessed under drought stress using microarray analysis previously [ 24 ], this type of evaluation has not been extended to other accessions/genotypes as of yet.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Differential Drought Response Mechanisms in Medicago sativa subsp. sativa and falcata through Comparative Assessments at the Physiological, Biochemical, and Transcriptional Levels

Singer

Subedi

Lehmann

et al. 2021

Plants

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Alfalfa (Medicago sativa L.) is an extensively grown perennial forage legume, and although it is relatively drought tolerant, it consumes high amounts of water and depends upon irrigation in many regions. Given the progressive decline in water available for irrigation, as well as an escalation in climate change-related droughts, there is a critical need to develop alfalfa cultivars with improved drought resilience. M. sativa subsp. falcata is a close relative of the predominantly cultivated M. sativa subsp. sativa, and certain accessions have been demonstrated to exhibit superior performance under drought. As such, we endeavoured to carry out comparative physiological, biochemical, and transcriptomic evaluations of an as of yet unstudied drought-tolerant M. sativa subsp. falcata accession (PI 641381) and a relatively drought-susceptible M. sativa subsp. sativa cultivar (Beaver) to increase our understanding of the molecular mechanisms behind the enhanced ability of falcata to withstand water deficiency. Our findings indicate that unlike the small number of falcata genotypes assessed previously, falcata PI 641381 may exploit smaller, thicker leaves, as well as an increase in the baseline transcriptional levels of genes encoding particular transcription factors, protective proteins, and enzymes involved in the biosynthesis of stress-related compounds. These findings imply that different falcata accessions/genotypes may employ distinct drought response mechanisms, and the study provides a suite of candidate genes to facilitate the breeding of alfalfa with enhanced drought resilience in the future.

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“…( Yang et al, 2019 ), Barley ( Li et al, 2010 ), Pigeon pea ( Sinha et al, 2016 ) etc. Usually, plant genomes harbor around twenty to fifty genes ( Gou et al, 2020 ). However, more than a hundred members have been reported in the genome of Brassica napus and Triticum aestivum ( Li et al, 2010 ; Chi et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive in silico Characterization of Universal Stress Proteins in Rice (Oryza sativa L.) With Insight Into Their Stress-Specific Transcriptional Modulation

et al. 2021

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In a world where climate change is real and its consequences are unprecedented, understanding of the plant adaptive capacity and native stress-responsive machinery is crucial. In recent years, universal stress proteins (USPs) have received much attention in the field of plant science due to their stress-specific transcriptional regulation. This study focuses on the extensive characterization of the USP gene family members in the monocot crop rice (Oryza sativa L. var. japonica). Here, we report a total of 44 USP genes in the rice genome. In silico characterization of these genes showed that domain architecture played a major role in the functional diversification of the USP gene family which holds for all plant USPs. On top of that, a higher conservation of OsUSP members has been exhibited with a monocot genome (Zea mays L.) as compared to a dicot genome (Arabidopsis thaliana L.). Expression profiling of the identified genes led to the discovery of multiple OsUSP genes that showed pronounced transcript alteration under various abiotic stress conditions, indicating their potential role as multi-functional stress-specific modules. Furthermore, expression validation of OsUSP genes using qRT-PCR provided a strong evidence for the utility OsUSP genes in building multi-stress tolerant plants. Altogether, this study provides leads to suitable USP candidates that could be targeted for plant breeding and genetic engineering experiments to develop stress resilient crop species.

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A Universal Stress Protein from Medicago falcata (MfUSP1) confers multiple stress tolerance by regulating antioxidant defense and proline accumulation

Cited by 24 publications

References 44 publications

Jasmonic acid (JA) and gibberellic acid (GA3) mitigated Cd-toxicity in chickpea plants through restricted cd uptake and oxidative stress management

Jasmonic acid (JA) and gibberellic acid (GA3) mitigated Cd-toxicity in chickpea plants through restricted cd uptake and oxidative stress management

Identification of Differential Drought Response Mechanisms in Medicago sativa subsp. sativa and falcata through Comparative Assessments at the Physiological, Biochemical, and Transcriptional Levels

Comprehensive in silico Characterization of Universal Stress Proteins in Rice (Oryza sativa L.) With Insight Into Their Stress-Specific Transcriptional Modulation

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