2021
DOI: 10.3390/vaccines9040308
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A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity

Abstract: There are seven viral serotypes of foot-and-mouth disease virus (FMDV): A, O, C, Asia 1, and Southern African Territories 1, 2, and 3 (SAT 1–3). Unlike serotype O FMDV vaccine strains, vaccine strains of serotype A FMDV do not provide broad-range cross-reactivity in serological matching tests with field isolates. Therefore, the topotype/lineage vaccine strain circulating in many countries and a highly immunogenic strain might be advantageous to control serotype A FMDV. We developed a new vaccine strain, A/SKR/… Show more

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Cited by 15 publications
(11 citation statements)
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“…Cell culture adaptation of such non-adapted strains leads to the selection of variants within the viral quasispecies that can utilize heparan sulphate (HS) proteoglycans (HSPG) as secondary receptors for infection [ 8 , 9 ]. To date, there have been numerous approaches to cell culture adaptions of FMDV strains, including sequential passaging within and between cell lines, and the use of reverse genetics to insert targeted mutations, informed from sequencing such passaged virus, into the external capsid-coding regions of the genome [ 10 , 11 , 12 , 13 ]. These approaches have had good success but are time-consuming and the targeted capsid mutations required for cell culture adaptation may be multiple and serotype or strain specific [ 10 ].…”
Section: Introductionmentioning
confidence: 99%
“…Cell culture adaptation of such non-adapted strains leads to the selection of variants within the viral quasispecies that can utilize heparan sulphate (HS) proteoglycans (HSPG) as secondary receptors for infection [ 8 , 9 ]. To date, there have been numerous approaches to cell culture adaptions of FMDV strains, including sequential passaging within and between cell lines, and the use of reverse genetics to insert targeted mutations, informed from sequencing such passaged virus, into the external capsid-coding regions of the genome [ 10 , 11 , 12 , 13 ]. These approaches have had good success but are time-consuming and the targeted capsid mutations required for cell culture adaptation may be multiple and serotype or strain specific [ 10 ].…”
Section: Introductionmentioning
confidence: 99%
“…A highly immunogenic vaccine is pivotal for protection against FMDV [ 38 ]. In the present study, the mean anti-structural protein antibody levels of the pigs and cows vaccinated with the rHN/TURVP1 and rHN vaccines were higher than those of the pigs vaccinated with the rHN/NXVP1 vaccine at 28–56 dpv, indicating that the replacement with the different VP1 proteins has a different effect on the immunological responses in animals, which may be attributed to the differences in the amino acids of the VP1 protein.…”
Section: Discussionmentioning
confidence: 99%
“…MG983730) and A/Yeoncheon/SKR/2017 (A YC, GenBank accession No. KY766148) were isolated by the Animal and Plant Quarantine Agency during FMD outbreaks in South Korea and adapted to BHK-21 suspension cells [ 17 , 18 ]. Recombinant A22 IRQ and O PA2 were constructed on the backbone of the O1 Manisa/Turkey/69 strain (GenBank accession No.…”
Section: Methodsmentioning
confidence: 99%