A validated high-performance liquid chromatographic method for the determination of glibenclamide in human plasma and its application to pharmacokinetic studies

Niopas, Ioannis; Daftsios, Athanasios C.

doi:10.1016/s0731-7085(02)00013-4

Cited by 38 publications

(30 citation statements)

References 12 publications

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“…To achieve peak blood concentration of the drug before the start of the study, glibenclamide was administered 45 minutes before baseline FMD assessment. 17 This was followed by the application of a 3-cycle RIPC stimulus on the contralateral arm, IR, and a final FMD assessment ( Figure 2, protocol a). To exclude a direct effect of glibenclamide on endothelial IR injury, the protocol was repeated without RIPC ( Figure 2, protocol b).…”

Section: Mechanism Of Protection By Ripc: Role Of K Atp Channelsmentioning

confidence: 99%

Transient Limb Ischemia Induces Remote Preconditioning and Remote Postconditioning in Humans by a K _ATP Channel–Dependent Mechanism

Loukogeorgakis¹,

Williams²,

et al. 2007

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Background— Transient limb ischemia administered before a prolonged ischemic insult has systemic protective effects against ischemia-reperfusion (IR) injury (remote ischemic preconditioning [RIPC]). It has been demonstrated that protection from IR can be achieved by brief periods of ischemia applied at a remote site during an injurious ischemic event (remote postconditioning [RPostC]). Using an in vivo model of endothelial IR injury, we sought to determine whether RPostC occurred in humans and whether it shared mechanistic similarities with RIPC. Methods and Results— Endothelial function was assessed by flow-mediated dilation before and after IR (20 minutes of arm ischemia followed by reperfusion). RIPC was induced by conditioning cycles of 5 minutes of ischemia and reperfusion on the contralateral arm or leg before IR. For RPostC induction, conditioning cycles were administered during the ischemic phase of IR. Oral glibenclamide was used to determine the dependence of RIPC and RPostC on K ATP channels. IR caused a significant reduction in flow-mediated dilation in healthy volunteers (baseline, 9.3±1.2% versus post-IR, 3.3±0.7%; P <0.0001) and patients with atherosclerosis (baseline, 5.5±0.6% versus post-IR, 2.3±0.5%; P <0.01). This reduction was prevented by RIPC (post-IR+RIPC: healthy volunteers, 7.2±0.5% [ P <0.0001 versus post-IR]; patients, 4.5±0.3% [ P <0.01 versus post-IR]) and RPostC (post-IR+RPostC: 8.0±0.5%; P <0.0001 versus post-IR). The protective effects of RIPC and RPostC were blocked by glibenclamide. Conclusions— This study demonstrates for the first time in humans that RPostC can be induced by transient limb ischemia and is as effective as RIPC in preventing endothelial IR injury. RIPC and RPostC share mechanistic similarities, with protection being dependent on K ATP channel activation. These results suggest that remote conditioning stimuli could be protective in patients with acute ischemia about to undergo therapeutic reperfusion.

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Section: Mechanism Of Protection By Ripc: Role Of K Atp Channelsmentioning

confidence: 99%

Transient Limb Ischemia Induces Remote Preconditioning and Remote Postconditioning in Humans by a K _ATP Channel–Dependent Mechanism

Loukogeorgakis¹,

Williams²,

et al. 2007

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“…[5][6][7] The matrices of health food are diverse and different from those of biosamples. Therefore analytical methods differ based on the biosample.…”

Section: Screening and Quantitative Analysis Formentioning

confidence: 99%

Screening and Quantitative Analysis for Sulfonylurea-Type Oral Antidiabetic Agents in Adulterated Health Food Using Thin-Layer Chromatography and High-Performance Liquid Chromatography

Kumasaka

Kojima

Honda

et al. 2005

JOURNAL OF HEALTH SCIENCE

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“…13 Different research has reported that glibenclamide has a low bioavailability, which is attributed to its poor dissolution properties. [14][15][16] Different methods have been reported to determine glibenclamide levels in various biological fluids, such as plasma 17,18 and serum, 19 in pharmaceutical formulation analyses [20][21][22] or in simultaneous determination of anti-diabetic drugs. 23 Our objectives were to formulate calcium-alginate beads containing glibenclamide by ionotropic gelation, characterize the resulting microparticles, evaluate the release characteristics of glibenclamide loaded calcium-alginate beads in an in vitro dissolution test, and compare it with two commercially available trademarks (Daonil ® and Glibetab ® ).…”

Section: Introductionmentioning

confidence: 99%

Encapsulation and release characteristics of glibenclamide loaded calcium-alginate beads

Charles-Navarro¹,

Bocanegra-García²,

Palos³

et al. 2010

Quím. Nova

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Recebido em 22/6/09; aceito em 17/3/10; publicado na web em 30/7/10The aims of this study were to formulate calcium-alginate beads containing glibenclamide, characterize the resulting microparticles, evaluate the release characteristics of this type of delivery system in an in vitro dissolution test, and compare it with two commercially available trademarks (Daonil ® and Glibetab ® ). We obtained glibenclamide loaded calcium-alginate beads with a rough surface and a particle size between 150-200 μm. For the in vitro dissolution test Daonil ® at 45 min showed a Q > 70%, whereas Glibetab ® and glibenclamide calciumalginate beads a Q < 70%; in spite of that glibenclamide calcium-alginate beads showed significant release properties.

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A validated high-performance liquid chromatographic method for the determination of glibenclamide in human plasma and its application to pharmacokinetic studies

Cited by 38 publications

References 12 publications

Transient Limb Ischemia Induces Remote Preconditioning and Remote Postconditioning in Humans by a K _ATP Channel–Dependent Mechanism

Transient Limb Ischemia Induces Remote Preconditioning and Remote Postconditioning in Humans by a K _ATP Channel–Dependent Mechanism

Screening and Quantitative Analysis for Sulfonylurea-Type Oral Antidiabetic Agents in Adulterated Health Food Using Thin-Layer Chromatography and High-Performance Liquid Chromatography

Encapsulation and release characteristics of glibenclamide loaded calcium-alginate beads

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A validated high-performance liquid chromatographic method for the determination of glibenclamide in human plasma and its application to pharmacokinetic studies

Cited by 38 publications

References 12 publications

Transient Limb Ischemia Induces Remote Preconditioning and Remote Postconditioning in Humans by a K ATP Channel–Dependent Mechanism

Transient Limb Ischemia Induces Remote Preconditioning and Remote Postconditioning in Humans by a K ATP Channel–Dependent Mechanism

Screening and Quantitative Analysis for Sulfonylurea-Type Oral Antidiabetic Agents in Adulterated Health Food Using Thin-Layer Chromatography and High-Performance Liquid Chromatography

Encapsulation and release characteristics of glibenclamide loaded calcium-alginate beads

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Product

Resources

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Transient Limb Ischemia Induces Remote Preconditioning and Remote Postconditioning in Humans by a K _ATP Channel–Dependent Mechanism

Transient Limb Ischemia Induces Remote Preconditioning and Remote Postconditioning in Humans by a K _ATP Channel–Dependent Mechanism