A Validated HPLC Method for Quantification and Optimization of Furocoumarins in Different Extracts of Fruits of Heracleum candicans

Govindarajan, R.; Singh, D. P.; Singh, Adarsh Pratap; Pandey, Madan Mohan; Rawat, Ajay Kumar Singh

doi:10.1365/s10337-007-0316-5

Cited by 16 publications

(11 citation statements)

References 13 publications

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“…The studies that have were limited in the number of furocoumarins studied or matrices investigated (12)(13)(14)(15)(16)(17)(18)(19). Frerot, et al published an elegant study of 15 furocoumarins (19).…”

Section: Methodsmentioning

confidence: 99%

An LC/MS/MS Method for the Analysis of Furocoumarins in Citrus Oil

Hiserodt

Chen

2012

ACS Symposium Series

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Furocoumarins are secondary plant metabolites commonly found in the families Umbelliferae and Rutaceae. The basic members of this class of compounds are psoralen and angelicin. Researchers have shown particular interest in alkoxy analogues of psoralen; particularly at the 5-and 8-positions. Bergapten (5-methoxypsoralen) and xanthotoxin (8-methoxypsoralen) have been found to be effective in the treatment of the skin diseases psoriasis and vitiligo when combined with the phototherapy technique, PUVA. A mode of action is thought to be mono-adduct formation with DNA but formation of di-adducts, leading to cross-linked DNA and potential carcinogenic side effects, is also a possibility. In response to this, the International Fragrance Association (IFRA) and the European Commission have agreed to ban the use of furocoumarins in cosmetic products except when they are part of natural essences. When present as the result of natural essences, the total furocoumarin concentration cannot exceed 5 ppm based on the analysis of seven marker furocoumarins. To ensure compliance, an LC/MS/MS method using electrospray ionization was developed for the analysis of the seven marker furocoumarins plus seven other furocoumarins. Various aspects of method development are discussed as well as conclusions from the analysis of citrus oils.

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Section: Methodsmentioning

confidence: 99%

An LC/MS/MS Method for the Analysis of Furocoumarins in Citrus Oil

Hiserodt

Chen

2012

ACS Symposium Series

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“…Structure of the analytes investigated in the present study (11), epoxybergamottin (15), with a maximum individual concentration of 1 mg/kg in a finished leave-on product, and suggested to limit the concentration at 50 mg/kg in rinse-off products. 14 To comply with regulations and low expected concentration limits, sensitive and specific analytical methods might be used.…”

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confidence: 88%

“…To comply with regulations and low expected concentration limits, sensitive and specific analytical methods might be used. High‐performance liquid chromatography coupled with a diode array detector (HPLC‐DAD) is the method of choice generally reported for their analysis . In 2004, Frérot et al proposed a reversed phase HPLC method with UV detection at 310 nm to separate and quantify the 15 most common furocoumarins ( 1 – 15 ) in essential oils .…”

Section: Introductionmentioning

confidence: 99%

“…Highperformance liquid chromatography coupled with a diode array detector (HPLC-DAD) is the method of choice generally reported for their analysis. [15][16][17][18][19][20] In 2004, Frérot et al proposed a reversed phase HPLC method with UV detection at 310 nm to separate and quantify the 15 most common furocoumarins (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) in essential oils. 18 This method was validated in a collaborative ring test by the IFRA Analytical Working Group 8 years later.…”

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confidence: 99%

“…32,41 Recently, HPTLC with a scanning system has stimulated a growing interest for the routine determination of target compounds. 46 Thus, the aim of this study was (i) to propose a new HPTLC method to analyse the 20 most common furocoumarins (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) found in perfumery raw materials for screening purposes ( Figure 1). and (ii) to propose a validated HPTLC method allowing the quantification of bergapten in essential oils in accordance with regulated limits.…”

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confidence: 99%

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HPTLC methods for qualitative and quantitative analysis of selected furocoumarins in essential oils

Borgne¹,

Cicchetti²,

Bertrand³

2017

Flavour & Fragrance J

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The concentration of furocoumarins, known to induce mutagenesis, carcinogenesis and photodermatitis under UV radiation, is limited by the EU cosmetic regulation in consumer products such as cosmetics, sun protection and bronzing formulations. The bergapten concentration is also limited by the International Fragrance Association. Analytical methods have been proposed for their quantification using high‐performance liquid chromatography (HPLC) coupled with UV detection or mass spectrometry with limits of detection (LOD) and quantification (LOQ) in accordance with the regulation. These methods are time and solvent consuming for routine quality control analyses. In this context, simple and fast HPLC methods in one‐ (1‐D) and two‐ (2‐D) dimensions were developed for the simultaneous analysis of the 15 most common furocoumarins reported in Citrus essential oils: psoralen, bergapten, xanthotoxin, isopimpinellin, oxypeucedanin, oxypeucedanin hydrate, byakangelicol, byakangelicin, heraclenin, 8‐geranyloxypsoralen, bergamottin, imperatorin, isoimperatorin, phellopterin, epoxybergamottin along with five other furocoumarins known to be present in Angelica, bergamot or Heracleum species: angelicin, bergaptol, isobergapten, psora‐4 and 5‐(4‐phenoxybutoxy)psoralen. The 1‐D method was validated for the quantification of bergapten using a Camag TLC Scanner as a detector. The LOD was determined at 2 mg/kg. The method was linear from 2 to 100 mg/kg and the method range was defined using accuracy profiles at with an 80% tolerance interval, with a 30% error, to be 5–100 mg/kg in acetonitrile and 13–100 mg/kg in matrix.

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Coumarins: Analytical and Preparative Techniques

Skalicka‐Woźniak

Głowniak

2014

Encyclopedia of Analytical Chemistry

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Coumarins are, nowadays, an important group of organic compounds from natural and synthetic sources, possessing a wide range of pharmacological activities. Coumarins comprise a large group of phenolic substances, which occur in plants as secondary metabolites. On account of the significance of this group of compounds, the development of effective methods for the extraction, separation, qualitative and quantitative analyses, and purification remains of high importance. The purpose of the chapter is to present information about methods of analysis and separation of this important group of naturally occurring compounds.

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A Validated HPLC Method for Quantification and Optimization of Furocoumarins in Different Extracts of Fruits of Heracleum candicans

Cited by 16 publications

References 13 publications

An LC/MS/MS Method for the Analysis of Furocoumarins in Citrus Oil

An LC/MS/MS Method for the Analysis of Furocoumarins in Citrus Oil

HPTLC methods for qualitative and quantitative analysis of selected furocoumarins in essential oils

Coumarins: Analytical and Preparative Techniques

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