A Validated Lc Method for Determination of Carbamazepine in Saliva

Abstract

Carbamazepine (CBZ) is a drug used as anticonvulsant, especially in the treatment of epilepsy. For adequate control disease, treatment must achieve adequate blood concentrations, therefore, it is very important to control the levels of anticonvulsant drugs in biological matrices with suitable analytical methods. A simple and fast liquid chromatographic method with UV detection was developed and validated for the determination of CBZ in saliva. Chromatographic separation was achieved with a RP-18 column, using acetonitrile and water with triethylamine at pH 7,3 as mobile phase in isocratic elution mode, at a flow rate of 1 mL/min. The detection was done at 230 nm. and bromazepam at 1,5 µg/mL was used as IS. The run time was 5 min. The described method was linear over a range of 0,5 -5,5 g/mL. The intraassay and interassay precision, expressed as the RSD, were in the range of 0,67 -2,93 % and 0,17 -9,33 %, respectively, the extraction recoveries were between 100,67 -100,69 %, the accuracy values ranged from -3,33 to 0,69 % and -2,67 to 0,72 in intraday and interday analysis, respectively, and the assay demonstrated adequate selectivity and specificity. The LLOQ is below the therapeutic level, demonstrating an adequate sensitivity of the method. The results showed that the proposed method was found to be suitable for quantitative determination of CBZ in saliva.

Assessment of adherence to carbamazepine using plasma and saliva samples, a study from Jordan

“…This study paints a compelling picture of the intricate interplay between saliva-based monitoring, non-adherence, and optimal carbamazepine therapy for Jordanian epilepsy patients, in contrast to the single approach employed in previously published studies [ 4 , 30 ]. The results of this study serve as guidance for healthcare facilities seeking to use saliva levels for the assessment of medication adherence.…”

“…In the present, HPLC was used to determine the level of carbamazepine concentration in saliva. De-Diego et al has recently developed a fast LC-UV analytical approach to determine carbamazepine level in saliva, using the RP-C18 column [ 30 ]. In agreement with our findings, a strong association was identified between serum and saliva carbamazepine concentrations.…”