A validated LC–MS/MS method for thyroid hormone determination in sea lamprey (Petromyzon marinus) plasma, gill, kidney and liver

Bussy, Ugo; Chung-Davidson, Yu Wen; Li, Ké; Fissette, Skye D.; Buchinger, Ethan G.; Li, Weiming

doi:10.1016/j.jchromb.2016.12.024

Cited by 14 publications

(9 citation statements)

References 42 publications

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“…The detection limits in plasma ranged between 29-42 pg/ml for T3 and 24-70 pg/ml for T4 [8,9] and recently 4.5-6 pg /ml for free T4 and 1.7-2.7 pg/ml for free T3 [32,33]. In tissues, detection limits of 1 pg/ml [14], 0.33 ng/ml [10] and 0.20-0.39 pg on-column [11] have been reported. Reported quantitation limits in tissues range from 10 pg/ ml (0.1 pg on-column) [14], 5-20 pg/ml (1.3-1.4 pg on-column) [11], 0.5-0.75 ng/ml (3.8-5.0 pg on-column) [13] to 1.1 ng/ml [10] for T3 and T4.…”

Section: Introductionmentioning

confidence: 96%

“…Previous studies have reported validation of LC-MS/MS methods for various THs and their metabolites, mostly for plasma, but also in some tissues [7][8][9][10][11][12][13][14]. Importantly, in biomedical and toxicological research using small animal models, and in ecophysiological research in wild species, the acquired sample volumes are likely to be very low and the small sample volume often has to be sufficient for multiple physiological measurements.…”

Section: Introductionmentioning

confidence: 99%

“…Kiebooms et al [32] and Tanoue et al [33] recently validated free T3 and T4 measurements in bovine serum. Kunisue et al [10], Ackermans et al [11], T Saba et al [12] and recently de Angelis et al [13] reported methods measuring THs and their metabolites in rat and mice tissues while Bussy et al [14] recently validated an UPLC method for tissues of sea lamprey. The detection limits in plasma ranged between 29-42 pg/ml for T3 and 24-70 pg/ml for T4 [8,9] and recently 4.5-6 pg /ml for free T4 and 1.7-2.7 pg/ml for free T3 [32,33].…”

Section: Introductionmentioning

confidence: 99%

“…In tissues, detection limits of 1 pg/ml [14], 0.33 ng/ml [10] and 0.20-0.39 pg on-column [11] have been reported. Reported quantitation limits in tissues range from 10 pg/ ml (0.1 pg on-column) [14], 5-20 pg/ml (1.3-1.4 pg on-column) [11], 0.5-0.75 ng/ml (3.8-5.0 pg on-column) [13] to 1.1 ng/ml [10] for T3 and T4. On-column amounts are not always explicitly stated, even if that would make comparisons easier [7].…”

Section: Introductionmentioning

confidence: 99%

“…in proteomics facilities. Nano-LC-MS/MS can provide similar quantitation limits as microflow [14,15], but uses even smaller injection volumes.…”

Section: Introductionmentioning

confidence: 99%

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A new method for measuring thyroid hormones using nano-LC-MS/MS

Ruuskanen

Hsu

Heinonen

et al. 2018

Journal of Chromatography B

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This paper describes a novel mass spectrometry based analytical method for analyzing thyroid hormones (THs). Thyroid hormones play a critical role in the regulation of many biological processes such as growth, metabolism and development. Several analytical methods using liquid chromatography-mass spectrometry (LC-MS) and tandem mass spectrometry (LC-MS/MS) have previously been developed to measure THs, especially in humans. For biomedical and toxicological research using small animal models, and in ecophysiological research using wild species where sample volume is limiting, sensitive methods are needed. In this study, we developed a nano-LC-MS/MS method enabling quantification of low concentrations of two key THs, thyroxine (T4) and 3,3',5-triiodothyronine (T3). The method was tested with egg yolk samples. We used a low flow rate (300 nl/min) to obtain maximal sensitivity of the method. The limit of quantitation was 10.6 amol for T4 and 17.9 amol for T3. The method shows good linearity (r > 0.99), repeatability and reproducibility (CVs <10%). We also reanalyzed yolk samples with radioimmunoassay for a comparison of the newly developed and previously used methods. Finally, we applied the methodology to measure hormones in egg yolk extracts in multiple avian species, and report interesting variation in maternal TH deposition. The newly developed nano-LC-MS/MS method is thus suitable for measuring THs in low concentrations and across species.

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Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…in proteomics facilities. Nano-LC-MS/MS can provide similar quantitation limits as microflow [14,15], but uses even smaller injection volumes.…”

Section: Introductionmentioning

confidence: 99%

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A new method for measuring thyroid hormones using nano-LC-MS/MS

Ruuskanen

Hsu

Heinonen

et al. 2018

Journal of Chromatography B

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Mass spectrometry in the diagnosis of thyroid disease and in the study of thyroid hormone metabolism

Borsò

Agretti

Zucchi

2020

Mass Spectrometry Reviews

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The importance of thyroid hormones in the regulation of development, growth, and energy metabolism is well known. Over the last decades, mass spectrometry has been extensively used to investigate thyroid hormone metabolism and to discover and characterize new molecules involved in thyroid hormones production, such as thyrotropin-releasing hormone. In the earlier period, the quantification methods, usually based on gas chromatography-mass spectrometry, were complicated and time consuming. They were mainly focused on basic research, and were not suitable for clinical diagnostics on a routine basis. The development of the modern mass spectrometers, mainly coupled to liquid chromatography, enabled simpler sample preparation procedures, and the accurate quantification of thyroid hormones, of their precursors, and of their metabolites in biological fluids, tissues, and cells became feasible. Nowadays, molecules of physiological and pathological interest can be assayed also for diagnostic purposes on a routine basis, and mass spectrometry is slowly entering the clinical laboratory. This review takes stock of the advancements in the field of thyroid metabolism that were carried out with mass spectrometry, with special focus on the use of this technique for the quantification of molecules involved in thyroid diseases. | INTRODUCTIONThyroid hormones (TH), namely triiodothyronine (3,5, 3′-triiodothyronine, T 3 ) and thyroxine (3,5,3′,5′-tetraiodothyronine, T 4 ), regulate development, energy metabolism, and growth, and their blood levels are controlled by complex central and peripheral signals mainly mediated by hypothalamic thyrotropin-releasing hormone (TRH) and pituitary thyrotropin (thyroid-stimulating hormone, TSH). The hypothalamic-pituitary-thyroid axis determines the set

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Thyroid Hormones and Derivatives: Endogenous Thyroid Hormones and Their Targets

Köhrle

2018

Methods in Molecular Biology

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More than a century after the discovery of L-Thyroxine, the main thyroid hormone secreted solely by the thyroid gland, several metabolites of this iodinated, tyrosine-derived ancestral hormone have been identified. These are utilized as hormones during development, differentiation, metamorphosis, and regulation of most biochemical reactions in vertebrates and their precursor species. Among those metabolites are the thyromimetically active 3,3',5-Triiodo-L-thyronine (T3) and 3,5-Diiodo-L-thronine, reverse-T3 (3,3',5'-Triiodo-L-thyronine) with still unclear function, the recently re-discovered thyronamines (e.g., 3-Iodo-thyronamine), which exert in part T3-antagonistic functions, the thyroacetic acids (e.g., Tetrac and Triac), as well as various sulfated or glucuronidated metabolites of this panel of iodinated signaling compounds. In the blood most of these hydrophobic metabolites are tightly bound to the serum distributor proteins thyroxine binding globulin (TBG), transthyretin (TTR), albumin or apolipoprotein B100. Cellular import and export of these charged, highly hydrophobic amino acid derivatives requires a number of cell-membrane transporters or facilitators such as MCT8 or MCT10 and members of the OATP and LAT families of transporters. Depending on their structure, the thyroid hormone metabolites exert their cellular action by binding and thus modulating the function of various receptors systems (e.g., ανβ3 integrin receptor and transient receptor potential channels (TRPM8) of the cell membrane), in part linked to intracellular downstream kinase signaling cascades, and several isoforms of membrane-associated, mitochondrial or nuclear thyroid hormone receptors (TR), which are members of the c-erbA family of ligand-modulated transcription factors. Intracellular deiodinase selenoenzymes, which obligatory are membrane integrated enzymes, ornithine decarboxylase and monoamine oxidases control local availability of biologically active thyroid hormone metabolites. Inactivation of thyroid hormone metabolites occurs mainly by deiodination, sulfation or glucuronidation, reactions which favor their renal or fecal elimination.

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A validated LC–MS/MS method for thyroid hormone determination in sea lamprey (Petromyzon marinus) plasma, gill, kidney and liver

Cited by 14 publications

References 42 publications

A new method for measuring thyroid hormones using nano-LC-MS/MS

A new method for measuring thyroid hormones using nano-LC-MS/MS

Mass spectrometry in the diagnosis of thyroid disease and in the study of thyroid hormone metabolism

Thyroid Hormones and Derivatives: Endogenous Thyroid Hormones and Their Targets

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