A validated method development for ketoprofen by a flow-injection analysis with UV-detection and its application to pharmaceutical formulations

Aboul‐Enein, Hassan Y.; Dal, Arın Gül; Tunçel, Muzaffer

doi:10.1016/s0014-827x(03)00061-2

Cited by 19 publications

(9 citation statements)

References 9 publications

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“…[24][25][26][27][28][29][30] FIA methods were also successfully applied in pharmaceutical assays carried out in the past. [31][32][33][34][35][36][37] This method has considerable time saving and has the superiority of high injection frequency which is very preferable for routine analysis. It also offers simple instrumentation and lower running cost.…”

Section: Discussionmentioning

confidence: 99%

Determination of Ticlopidine in Pharmaceutical Tablets by Flow Injection Analysis Using UV-Detection

Can

Altıokka

Aboul‐Enein

2008

Journal of Liquid Chromatography & Related Technologies

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A precise and accurate flow injection analysis method for the quantification of ticlopidine in pharmaceuticals is described. An aqueous carrier stream, which was entirely prepared with water was chosen for the flow injection analysis. The method development was achieved by using a reference standard solution of ticlopidine at 3.19 Â 10 À6 M concentration, which was prepared in water. The solution was injected into the instrumental system at a flow rate of 1.0 mL Á min À1 and signals were detected by a UV detector at 214.2 nm. The calibration curves of TP were linear in the concentration range of 1.59 Â 10 À6 À 7.99 Â 10 À5 M. The intra-and inter-assay precision was less than 1.3% relative standard deviation. The method exhibited good linearity with the correlation coefficients close to unity. The limit of detection and limit of quantization concentrations were found to be 8.91 Â 10 À8 and 2.70 Â 10 À7 M, respectively. The effects of the tablet excipients were insignificant at the 95% probability level. The calculated tablet contents were around 99%, which is in agreement with the ranges stated by pharmacopoeias.

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Section: Discussionmentioning

confidence: 99%

Determination of Ticlopidine in Pharmaceutical Tablets by Flow Injection Analysis Using UV-Detection

Can

Altıokka

Aboul‐Enein

2008

Journal of Liquid Chromatography & Related Technologies

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“…Potency studies: Though titrimetric method has recently been used to determine the potency of ketoprofen bulk (Maheswari et al, 2010) potency of ketoprofen solid dosage form is reported to be estimated accurately by spectrophotometry (Blanco et al, 1997;Aboul-Enein et al, 2003). A UV spectrophotometer (Camspec, UK) was used to determine the amount of ketoprofen present in the samples at 258 nm and was compared with the standard sample of that drug.…”

Section: Methodsmentioning

confidence: 99%

Quality evaluation of Ketoprofen Solid Dosage Forms Available in the Pharma-Market of Bangladesh

Fatemeh¹

2010

American Journal of Applied Sciences

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Problem statement: Ketoprofen, a widely used analgesic and anti-inflammatory drug, was available in two types of solid dosage forms in the pharma-market of Bangladesh: enteric-coated tablet and capsule of sustained-release pattern. Seven brands of ketoprofen enteric-coated tablets and four brands of ketoprofen sustained release capsules were studied for their in-vitro release behavior as well as potency status. Approach: The studies were carried out to compare with standard sample of ketoprofen sustained-release pellets and that of ketoprofen powder. To determine the release pattern of the preparations, disintegration study and dissolution tests were performed as per the method described in standard pharmacopeial compendia (British Pharmacopoeia or BP and United State Pharmacopoeia or USP respectively). The potency of the samples were determined by the UV spectroscopic method as described in BP. Results: Out of seven samples of tablets, two brands (KT-03 and KT-07) were found noncompliant in respect of disintegration test in acid stage whereas rest of the brands complied with BP specification in buffer stage at pH 6.8. The dissolution study of ketoprofen tablets were carried out in both acid and buffer stages and all the samples satisfied with USP specification in both stages. All of the brands of ketoprofen capsule also complied with the USP specification. Two brands (KT-03 and KT-07) of tablets were found non-compliant whereas rest brands of tablets and all brands of capsules exerted compliance in respect of potency. Conclusion: This study will provide a basis for further invivo bioavailability studies of these brands to draw a more conclusive remark regarding quality status of these samples.

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“…Some techniques includes capillary zone electrophoresis 4 , UV-spectrophotometry [5][6][7][8] , highperformance liquid chromatography [9][10][11][12] , flow injection technique with hemiluminiscence 13,14 , flow injection with UV-detection 15 , polarography 16 ODS of 10cm X 4.6 mm column, (5μm particle size). The mobile phase, degassed under vacuum in an ultrasonic bath, consists of 0.01 M sodium dihydrogen phosphate (adjusted to pH 6.5 with orthophosphoric acid), methanol and acetonitrile, 4:4:3 (v/v), respectively.…”

Section: Introductionmentioning

confidence: 99%

Untitled

2016

IJPSR

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A simple and accurate Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the estimation of ketoprofen in vitro samples and in vivo samples from bulk drug and from administered transdermal dosage form, using Spherosorb S5 ODS of 10cm X 4.6 mm column, (5μm particle size). Mobile phase for in vitro sample analysis and in vivo plasma samples consists of 0.01 M sodium dihydrogen phosphate (pH adjusted to 6.5 with ortho phosphoric acid), methanol, and acetonitrile, 4:3:3 (v/v) respectively. Isocratic elution technique was followed. The flow rate was 0.5ml/min and the detection was monitored out by UV detector at 265nm. The retention time for ketoprofen was found to be 2.982 in in vitro sample and 3.025 min in in vivo sample. Naproxen was used as internal standard for in vivo sample analysis. The proposed method has permitted the quantification of ketoprofen over linearity in the range of 100-1000 ng/ml.

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A validated method development for ketoprofen by a flow-injection analysis with UV-detection and its application to pharmaceutical formulations

Cited by 19 publications

References 9 publications

Determination of Ticlopidine in Pharmaceutical Tablets by Flow Injection Analysis Using UV-Detection

Determination of Ticlopidine in Pharmaceutical Tablets by Flow Injection Analysis Using UV-Detection

Quality evaluation of Ketoprofen Solid Dosage Forms Available in the Pharma-Market of Bangladesh

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