A validated quantitative HPTLC method for analysis of biomarkers in<i>Ficus carica</i>L.

Singh, Adarsh Pratap; Singh, D. P.; Srivastava, Sharad; Govindarajan, R.; Rawat, Ajay Kumar Singh

doi:10.1556/jpc.20.2007.6.8

Cited by 10 publications

(3 citation statements)

References 10 publications

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“…Modern instruments utilize wavelengths from 190 to 800 nm in single or multi-wavelength scans and can also record spectra [45]. Results obtained this way can be compared to those from modern photo diode array detectors in HPLC [46], [47], [48], [49]. Also images of the plate can be quantitatively evaluated.…”

Section: Detection and Evaluationmentioning

confidence: 99%

Plant Analysis 2008 – Planar Chromatography

Reich¹,

Widmer²

2008

Planta Med

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Section: Detection and Evaluationmentioning

confidence: 99%

Plant Analysis 2008 – Planar Chromatography

Reich¹,

Widmer²

2008

Planta Med

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“…Furocoumarin separation by HPTLC was reported both on a normal or reversed phase, in one‐ or two‐dimensions, but recent methods were preferentially developed in a normal phase . Table shows an overview of recently published methods used to analyse or isolate furocoumarins by HPTLC.…”

Section: Introductionmentioning

confidence: 99%

“…21 The method was shown to be linear between 10 and 250 mg kg −1 with a LOD and an LOQ of 2.5 and 10 mg kg −1 , respectively. The method was only validated in a standardized essential oils matrix because of the lack of specificity of the UV Furocoumarin separation by HPTLC was reported both on a normal or reversed phase, in one- [24][25][26][27][28][29][30][31][32] or two-dimensions, [33][34][35][36][37] but recent methods were preferentially developed in a normal phase. 27,30,38 45 in the fruits of H. candicans.…”

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confidence: 99%

HPTLC methods for qualitative and quantitative analysis of selected furocoumarins in essential oils

Borgne¹,

Cicchetti²,

Bertrand³

2017

Flavour & Fragrance J

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The concentration of furocoumarins, known to induce mutagenesis, carcinogenesis and photodermatitis under UV radiation, is limited by the EU cosmetic regulation in consumer products such as cosmetics, sun protection and bronzing formulations. The bergapten concentration is also limited by the International Fragrance Association. Analytical methods have been proposed for their quantification using high‐performance liquid chromatography (HPLC) coupled with UV detection or mass spectrometry with limits of detection (LOD) and quantification (LOQ) in accordance with the regulation. These methods are time and solvent consuming for routine quality control analyses. In this context, simple and fast HPLC methods in one‐ (1‐D) and two‐ (2‐D) dimensions were developed for the simultaneous analysis of the 15 most common furocoumarins reported in Citrus essential oils: psoralen, bergapten, xanthotoxin, isopimpinellin, oxypeucedanin, oxypeucedanin hydrate, byakangelicol, byakangelicin, heraclenin, 8‐geranyloxypsoralen, bergamottin, imperatorin, isoimperatorin, phellopterin, epoxybergamottin along with five other furocoumarins known to be present in Angelica, bergamot or Heracleum species: angelicin, bergaptol, isobergapten, psora‐4 and 5‐(4‐phenoxybutoxy)psoralen. The 1‐D method was validated for the quantification of bergapten using a Camag TLC Scanner as a detector. The LOD was determined at 2 mg/kg. The method was linear from 2 to 100 mg/kg and the method range was defined using accuracy profiles at with an 80% tolerance interval, with a 30% error, to be 5–100 mg/kg in acetonitrile and 13–100 mg/kg in matrix.

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Current Awareness in Phytochemical Analysis

2008

Phytochemical Analysis

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley &Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of phytochemical analysis. Each bibliography is divided into 13 sections: 1 Reviews; 2 General; 3 Nucleic Acids; 4 Amino Acids, Proteins &Enzymes; 5 Carbohydrates; 6 Lipids; 7 Secondary Products; 8 Growth Regulators; 9 Industrially‐Important Products; 10 Toxins/Allergens; 11 Pigments; 12 Vitamins; 13 Others. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted.

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A validated quantitative HPTLC method for analysis of biomarkers inFicus caricaL.

Cited by 10 publications

References 10 publications

Plant Analysis 2008 – Planar Chromatography

Plant Analysis 2008 – Planar Chromatography

HPTLC methods for qualitative and quantitative analysis of selected furocoumarins in essential oils

Current Awareness in Phytochemical Analysis

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