Automotive research and development passed through a vast evolution during past decades. Many passive and active driver assistance systems were developed, increasing the passengers' safety and comfort. This ongoing process is a main focus in current research and offers great potential for further systems, especially focusing on the task of autonomous and cooperative driving in the future. For that reason, information about the current stability in terms of dynamic behavior and vehicle environment are necessary for the systems to perform properly. Thus, model-based online state and parameter estimation have become important throughout the last years using a detailed vehicle model and standard sensors, gathering this information. In this chapter, state and parameter estimation in vehicle dynamics utilizing the unscented Kalman filter is presented. The estimation runs in real time based on a detailed vehicle model and standard measurements taken within the car. The results are validated using a Volkswagen Golf GTE Plug-In Hybrid for various dynamic test maneuvers and a Genesys Automotive Dynamic Motion Analyzer (ADMA) measurement unit for highprecision measurements of the vehicle's states. Online parameter estimation is shown for friction coefficient estimation performing maneuvers on different road surfaces.