A Versatile and Efficient Method to Isolate DNA–Polymer Conjugates

Abstract

We present a facile and adaptable method to purify and isolate DNA−polymer conjugates from different uncharged homo, random, or block copolymer families. Anion exchange chromatography is used to separate the reaction solution and retrieve the excess unreacted polymer and oligonucleotide. The stationary phase has a high efficiency (25 nmol of DNA per run), facilitating the purification of large batches without compromising the peak shape and resolution. To demonstrate the versatility of this method, different types of polymers, including acrylates, methacrylates, and acrylamides containing hydrophilic and hydrophobic blocks, were purified with high yields. Additionally, DNA−polymer conjugates with various DNA block lengths were also successfully purified, further highlighting the broad applicability of this method.

Natural and Synthetic Polymers for Biomedical and Environmental Applications

“…In this context, drug delivery systems (DDSs) constructed using representative natural polymers such as polysaccharides (chitosan and hyaluronic acid) and proteins (silk fibroin and collagen) are generally summarized. These DDS systems are used to deliver payloads, which mainly include low molecular weight drugs, proteins, and DNA, and are employed for various applications such as tissue engineering, wound healing, or anticancer therapy [ 55 , 56 ]. Moreover, a DDS constructed by modified biopolymers has also been presented, focusing on the chemical and morphological modifications, the additions of smart stimuli-triggered or targeted motifs, and so on, which promoted delivery and therapy efficiency.…”