A Versatile and Robust Platform for the Scalable Manufacture of Biomimetic Nanovaccines

Abstract

Biomimetic strategies are useful for designing potent vaccines. Decorating a nanoparticulate adjuvant with cell membrane fragments as the antigen‐presenting source exemplifies, such as a promising strategy. For translation, a standardizable, consistent, and scalable approach for coating nanoadjuvant with the cell membrane is important. Here a turbulent mixing and self‐assembly method called flash nanocomplexation (FNC) for producing cell membrane‐coated nanovaccines in a scalable manner is demonstrated. The broad applicability of this FNC technique compared with bulk‐sonication by using ten different core materials and multiple cell membrane types is shown. FNC‐produced biomimetic nanoparticles have promising colloidal stability and narrow particle polydispersity, indicating an equal or more homogeneous coating compared to the bulk‐sonication method. The potency of a nanovaccine comprised of B16‐F10 cancer cell membrane decorating mesoporous silica nanoparticles loaded with the adjuvant CpG is then demonstrated. The FNC‐fabricated nanovaccines when combined with anti‐CTLA‐4 show potency in lymph node targeting, DC antigen presentation, and T cell immune activation, leading to prophylactic and therapeutic efficacy in a melanoma mouse model. This study advances the design of a biomimetic nanovaccine enabled by a robust and versatile nanomanufacturing technique.

A nanoparticulate dual scavenger for targeted therapy of inflammatory bowel disease

“…Here, we used MON with good biodegradability and with a high surface area for greater PEI conjugation and cfDNA binding. Our group recently developed diselenide-bridged MON with a large pore size and a ROS-degradable matrix for controlled drug release ( 35 , 36 ) . We hypothesized that these MON may serve not only as a core for PEI functionalization to scavenge cfDNA but also as a ROS scavenger via cleavage of the diselenide bonds and subsequent depletion of ROS.…”

Self-Assembly Engineering Nanodrugs Composed of Paclitaxel and Curcumin for the Combined Treatment of Triple Negative Breast Cancer

“…Significant work has been dedicated to developing drug delivery system that can concurrently deliver PTX and Cur to the tumor site, which might provide improved therapeutic activity and safety. However, most reported carriers have limited loading capacity and there are also concerns regarding their possible toxicity and biodegradation (Hiremath et al, 2019;Li et al, 2019;Zhao et al, 2019;Shao et al, 2020;Xiong et al, 2020;Hu et al, 2021;Liao et al, 2021;Zhang et al, 2021). Carrierfree drug delivery systems are recently developed alternatives that do not rely on inert carriers and thus avoid potential toxicity.…”

Biocompatible Supramolecular Mesoporous Silica Nanoparticles as the Next-Generation Drug Delivery System

“…Largely dependent on the route of drug administration, the aforementioned are due to insufficient stability of the DDS carriers in vivo , poor bioavailability and solubility of drugs, and lack of specificity by targeting ligand. Various nanoparticles including mesoporous silica-based, decorated, coated, cloaked, or linked with stimuli-responsive materials and some with targeting ligands have been synthesized to overcome the challenges ( Wang et al, 2019 ; Yan et al, 2019 ; Hu et al, 2021 ; Zhang et al, 2021 ; Yang et al, 2022 ). Recently, an improved performance, targeted delivery of mesoporous organosilica nanoparticles (MONs) has been designed to be immunogenic by coating it with 4T1 breast cancer cell membrane while responsive to X-ray as the external activator that controlled the drug release ( Shao et al, 2020 ).…”