A Versatile Giemsa Protocol for Permanent Nuclear Staining of Fungi

Wilson, A. D.

doi:10.2307/3760326

Cited by 11 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, there was considerable divergence between the copies in each isolate. The 560-bp 5' regions of tub2-2, tub2-3, and tub24 differed by [14][15][16][17][18][19][20][21][22][23][24][25][26] nucleotide substitutions, a level comparable to their 17-35 differences with tub2 of E. typhina isolate E8 (15) Fig. 4.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Evolutionary diversification of fungal endophytes of tall fescue grass by hybridization with Epichloë species.

Tsai

Liu

Stäben

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

247

137

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

“…Nuclear staining was by the method of Wilson (23). Southern blot hybridization was as described (24), but the digoxigenin-labeled hybridization probe was generated by PCR (25).…”

mentioning

confidence: 99%

Evolutionary diversification of fungal endophytes of tall fescue grass by hybridization with Epichloë species.

Tsai

Liu

Stäben

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

247

137

View full text Add to dashboard Cite

show abstract

“…Ascospores ejected onto glass slides coated with Haupt's adhesive (Jensen, 1962) were also stained by the HCI-Giemsa method (Wilson, 1992) after fixation in Farmer's fluid (Sass, 1951) for lOmin.…”

Section: Methodsmentioning

confidence: 99%

Sclerotinia nivalis, sp. nov., the pathogen of snow mold of herbaceous dicots in northern Japan

Saito¹

1997

Mycoscience

View full text Add to dashboard Cite

A new Sc/erotinia, previously reported as S. intermedia from Japan, is described as Sclerotinia nivalis on the morphological basis of the sclerotial anamorph and teleomorph produced in culture. The characters assigning this species to the genus Sclerotinia are the tuberoid sclerotia superficially produced on suscepts, the small sclerotia produced on aerial mycelium in culture, the interhyphal spaces in medullary tissue of sclerotia, and the globose cells constructing the ectal excipulum of apothecia. It is distinguishable from S. sclerotiorum, S. minor, and S. trifoliorum by the intermediate sized sclerotia in culture, binucleate ascospores, the molecular mass of major proteins of sclerotia, and the patterns of esterase isozymes in sclerotial extracts. Although S. nivalis causes snow mold of various dicots, it is a mesophile having an optimum temperature for mycelial growth of around 20~It attacks edible burdock (Arctium lappa), Chryhsanthemum morifolium, Ambrosia elatior, carrot (Daucus carota), Angelica acutiloba, Ajuga reptans, and Plantago lanceolata.Key Words herbaceous dicots; mesophilic fungus; Sclerotinia; snow mold.Tochinai and Sugimoto (1958) studied a snow-mold disease of santonica (Artemisia maritima L., the source plant for santonin industry) caused by a sclerotiniaceous fungus, which had been the major problem for santonica cultivation in Hokkaido since the early 1940s. They identified the causal organism with Sclerotinia intermedia Ramsey, which was synonymized with Sclerotinia sclerotiorum (Lib.) de Bary by Purdy (1955) proposing a broader concept of the species S. sclerotiorum based on variability of morphological characters that had been used for identification. The characters of the organism that Tochinai and Sugimoto adopted in their identification are the color of mycelium on the suscept (not always white), temperature response in mycelial growth (faster growth at 0~ than S. sclerotiorum and S. trifoliorum Erikss.), intermediate size of sclerotia produced at 25 ~ (1.9-4.3mm) and measurements of asci (118.5x 7.3/~m) and ascospores (10.5x4.5~m).Later, autumn-sown rape (Brassica campestris L. subsp, napus Hook.) and summer-sown carrot (Daucus carota L.) left unharvested in the field were found to be damaged by the same organism under the similar conditions as with santonica (Sugimoto et al., 1962;Narita, 1980). Although there have been no published records, the organism found by Tochinai and Sugimoto was also considered to cause snow-mold disease of pyrethrum (Chrysanthemum cinerariaefolium Bocc.), the source plant for pyrethrin industry, which was widely cultivated in Hokkaido in the 1930s (Narita, 1980). Thereafter, the organism has become rare in agricultural sites in Hokkaido because of the decline of cultivation of perennial or biennial dicot crops which could be suscepts. On the other hand, the recent taxonomic revision of Sclerotiniaceae (Kohn, 1979) that synonymized S. intermedia with Sclerotinia minor Jagger led to the re-examination of the organism designated as S. intermedia in Japan,...

show abstract

“…For the purpose of staining nuclei in ascospores, a fresh apothecium was placed to make its upper face contact with a glass slide, allowing ascospores to be ejected onto the slide. The ascospores were fixed in Carnoi's fluid (Sass 1951) and stained with the HCl-Giemsa technique (Wilson 1992). Observations of the tissue structure of apothecia and sclerotia were carried out for the resinembedded sections prepared by the following methods: tissues of apothecia and sclerotia were fixed and stored in 4% (v/v) glutaraldehyde solution in 1/15 M phosphate buffer, pH 7.0 at 3°C, then dehydrated with an acetone series, embedded in Spurr's low-viscosity resin, and sectioned in 1-2 µm thickness with glass knives on a Porter Blum MT 1 microtome.…”

Section: Microscopymentioning

confidence: 99%

Ciborinia gentianae sp. nov., the causal organism of sclerotial flower blight of cut-flower gentians

Saito

Kaji²

2006

Mycoscience

View full text Add to dashboard Cite

A new Ciborinia causing sclerotial flower blight of cut-flower gentians (Gentiana triflora var. japonica and interspecific hybrids between related species or varieties) is described as Ciborinia gentianae on the morphological basis of sclerotia and apothecia. The characteristics of Ciborinia gentianae are (1) an abundant production of spermodochia in the hollow cavity of host stems; (2) flat and thin sclerotia produced beneath the epidermis and the inclusion of host vascular remnants within their medulla; (3) globose cells composed of ectal excipulum of apothecia; (4) elongated cells with a slight apical swelling in ectal excipulum at the apothecial margin; and (5) tetra nucleate ascospores. Asci and ascospores mounted in Melzer's reagent measured 156-208 × 8-12 µm and 11.8-15 × 5.5-7.1 µm, respectively.

show abstract

A Versatile Giemsa Protocol for Permanent Nuclear Staining of Fungi

Cited by 11 publications

References 18 publications

Evolutionary diversification of fungal endophytes of tall fescue grass by hybridization with Epichloë species.

Evolutionary diversification of fungal endophytes of tall fescue grass by hybridization with Epichloë species.

Sclerotinia nivalis, sp. nov., the pathogen of snow mold of herbaceous dicots in northern Japan

Ciborinia gentianae sp. nov., the causal organism of sclerotial flower blight of cut-flower gentians

Contact Info

Product

Resources

About