This paper introduces a powerful LabVIEW-based toolbox for monitoring, diagnosis and management of vital biosignals [electrocardiogram (ECG) and electromyogram (EMG)]. The main features of this software present in user-friendly graphical user interface, the patient's data management, real-time monitoring of heart and muscles activities, reviewing patient's progress through variety of charts, diagnosis of heart and muscles disorders and data transmission to remote clinician. The experimental validation of this toolbox showed that it has good performance and demonstrated a superior assurance quality in optimising clinical decisions based on real-time analysis of patient's vital signals.Reference to this paper should be made as follows: Amer, M.M.B., Qtait, Y., Al-Ebbini, L., Ammary, S. and Awwad, M. (2010) 'Design of a user-friendly LabVIEW-based toolbox for real-time monitoring and diagnosis of vital signals', Int.