A versatile method for the determination of serum cortisol binding globulin and sex hormone binding globulin binding capacities

Hammond, Geoffrey L.; Lähteenmäki, Pekka

doi:10.1016/0009-8981(83)90237-1

Cited by 215 publications

(103 citation statements)

References 19 publications

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“…Serum SHBG levels were determined using an established steroid-binding capacity assay using reference serum samples to control for inter-assay performance (Hammond and Lähteenmäki, 1983). The percentages of free oestradiol in serum samples were estimated from a nomogram describing the relationship between serum SHBG levels and percentages of free oestradiol measured using the centrifugal ultrafiltration-dialysis method in a reference population of normal weight pre-and postmenopausal women (Langley et al, 1985).…”

Section: Measurement Of Blood Samplesmentioning

confidence: 99%

The association of breast mitogens with mammographic densities

et al. 2002

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Radiologically dense breast tissue (mammographic density) is strongly associated with risk of breast cancer, but the biological basis for this association is unknown. In this study we have examined the association of circulating levels of hormones and growth factors with mammographic density. A total of 382 subjects, 193 premenopausal and 189 postmenopausal, without previous breast cancer or current hormone use, were selected in each of five categories of breast density from mammography units. Risk factor information, anthropometric measures, and blood samples were obtained, and oestradiol, progesterone, sex hormone binding globulin, growth hormone, insulin-like growth factor-I and its principal binding protein, and prolactin measured. Mammograms were digitised and measured using a computer-assisted method. After adjustment for other risk factors, we found in premenopausal women that serum insulin-like growth factor-I levels, and in postmenopausal women, serum levels of prolactin, were both significantly and positively associated with per cent density. Total oestradiol and progesterone levels were unrelated to per cent density in both groups. In postmenopausal women, free oestradiol (negatively), and sex hormone binding globulin (positively), were significantly related to per cent density. These data show an association between blood levels of breast mitogens and mammographic density, and suggest a biological basis for the associated risk of breast cancer.

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Section: Measurement Of Blood Samplesmentioning

confidence: 99%

The association of breast mitogens with mammographic densities

et al. 2002

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“…CBG assay CBG levels were measured by a radioligand method (Hammond & Lahteenmaki 1983). Briefly, endogenous steroids were removed by treating 10 µl of plasma with dextran coated charcoal (DCC, 0·2% charcoal, 0·02% dextran) at room temperature for 30 min.…”

Section: Hormone Assaysmentioning

confidence: 99%

Increased corticosteroid binding capacity of plasma albumin but not of corticosteroid-binding globulin caused by ACTH-induced changes in free fatty acid concentrations in snowshoe hares and rabbits

Boonstra

Tinnikov²

1998

Journal of Endocrinology

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Free fatty acids (FFAs) are rapidly mobilized by ACTH and have been shown to be potent endogenous modulators of steroid-protein interactions. We increased FFA in lagomorphs by ACTH and then separated the transient increase in glucocorticoid binding capacity of plasma into that accounted for by changes in binding to albumin and to corticosteroid-binding globulin (CBG). Sequential injections of dexamethasone and ACTH into both snowshoe hares and laboratory rabbits resulted in the rapid mobilization of FFA only after the ACTH injection. The maximum corticosteroid binding capacity increase paralleled that of the FFA increase in both species. In rabbits, CBG levels remained constant over the duration of the experiment. Corticosterone binding by rabbit albumin increased in a dose-dependent fashion in response to increases in FFA (oleic and linoleic acid) concentrations. Finally, by stimulating FFA release in snowshoe hares with ACTH and separating the increase in corticosteroid binding capacity through selective denaturing of CBG by heat, we determined that the increase in plasma binding capacity was a response to changes in binding by albumin, not CBG. Thus FFA released in response to stressors in lagomorphs may effect short-term increases in steroid binding.

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“…SHBG was measured by the dextran-coated charcoal method. 30 Total testosterone was measured by a coated tube commercial radioimmunoassay (Diagnostic Products Corporation, Los Angeles, CA), and total estradiol was measured by a commercial double antibody radioimmunoassay (Pantex, Santa Monica, CA). Percent free testosterone was calculated from the SHBG and total testosterone concentrations by using a computer program that calculates the equilibrium distribution of steroid hormones according to the law of mass action.…”

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confidence: 99%

Association of decreased sex hormone binding globulin and cardiovascular risk factors.

et al. 1989

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Sex hormones play a major role in determining the risk of cardiovascular disease. While earlier studies have shown that reduced sex hormone binding globulin (SHBG) Is associated with increased glucose and Insulin concentrations in premenopausal women, few data exist on the relationship of SHBG to other cardiovascular risk factors in women. We hypothesized that decreased SHBG would be associated with an atherogenlc pattern of cardiovascular risk factors. We measured total testosterone, total estradlol and SHBG, llplds and lipoproteins, glucose and insulin, and systolic and dlastolic blood pressure In 96 premenopausal women. Although total testosterone and total estradiol were not related to cardiovascular risk factors, SHBG was negatively associated with trlglycerlde concentration (r=-0.37) and positively associated with high density llpoproteln cholesterol (HDLC) (r=0.42). After adjustment for overall adiposity (body mass index) and upper body adiposity (as measured by the ratio of waist-to-hlp circumferences), SHBG was still positively related to HDLC, but not to trlglycerlde. Adjustment for Insulin abolished the relationship between SHBG and triglyceride levels, but did not alter the relationship between SHBG and HDLC. Sex hormones were not related to either systolic or dlastolic blood pressure. (Arteriosclerosis 9:136-143, January/February 1989) S ex hormones play a major role in determining the risk of cardiovascular disease. Indirect evidence for this concept is provided by the higher triglyceride and low density lipoprotein cholesterol (LDLC) and lower high density lipoprotein cholesterol (HDLC) levels in men than in women.1 More direct evidence for the role of sex hormones as determinants of cardiovascular risk factors is provided by studies showing that exogenous androgen administration increases LDLC and decreases HDLC. 23 Conversely, administration of estrogens to postmenopausal women with familial hypercholesterolemia is associated with decreased LDLC and increased HDLC. 4 Sex hormone binding globulin (SHBG) may also be an important predictor of lipids and lipoproteins, since it is the major determinant of the ratio of free to bound plasma testosterone and other androgens. 5 Although decreased SHBG and increased percent free testosterone have been associated with increased glycemia and insulinemia in premenopausal women, 67 few data exist on the relationship of endogenous sex hormones and SHBG to lipids and lipoproteins. Studies to date suggest that SHBG is positively associated with HDLC in both premenopausal 8 and postmenopausal 9 women.

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A versatile method for the determination of serum cortisol binding globulin and sex hormone binding globulin binding capacities

Cited by 215 publications

References 19 publications

The association of breast mitogens with mammographic densities

The association of breast mitogens with mammographic densities

Increased corticosteroid binding capacity of plasma albumin but not of corticosteroid-binding globulin caused by ACTH-induced changes in free fatty acid concentrations in snowshoe hares and rabbits

Association of decreased sex hormone binding globulin and cardiovascular risk factors.

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