A Versatile Photocrosslinkable Silicone Composite for 3D Printing Applications

Alioglu, Mecit Altan; Yilmaz, Yasar Ozer; Gerhard, Ethan Michael; Pal, Vaibhav; Gupta, Deepak; Rizvi, Syed Hasan Askari; Ozbolat, Ibrahim T.

doi:10.1002/admt.202301858

Cited by 5 publications

(3 citation statements)

References 89 publications

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“…To assess resolution, two key parameters are examined: the thickness of printed lines and the clarity of the edges of hexagonal structures which is reported in Figure 4 to increased light scattering and, consequently, a potential decrease in printing resolution [66,67]. This phenomenon might explain the observed increase in branch thickness in structures printed with 5G1d ink after 1 minute of crosslinking, which was approximately 868 ± 181.48 μm.…”

Section: Printability Evaluationmentioning

confidence: 99%

3D bioprinting of Liver Microenvironment Model Using Photocrosslinkable Decellularized Extracellular Matrix based Hydrogel

Tabatabaei Rezaei,

Kumar,

Liu

et al. 2024

Preprint

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The liver, as one of the vital organs in the body, plays a crucial role in various bodily functions. Numerous factors can cause liver damage, that the sole remedy for severe liver conditions is the transplantation of healthy liver tissue. In response to the transplantation challenges, innovative approaches involving hydrogel-based technologies have emerged, leading to the creation of highly functionalized tissues. The development of three-dimensional printing and patterning of cell-laden biomaterial matrices offers promising advances for creating tissue-specific structures in tissue engineering and bioprinting. However, the matrix materials currently employed in bioprinting liver microtissue often fail to capture the complexity of the natural extracellular matrix (ECM), hindering their ability to restore innate cellular shapes and functions. Liver ECM-based hydrogels are increasingly recognized for their potential as biomimetic 3D cell culture systems that facilitate the exploration of liver disease, metabolism, and toxicity mechanisms. Yet, the conventional production of these hydrogels relies on slow thermal gelation processes, which restrict the manipulation of their mechanical characteristics. In this research, we introduce a novel approach with a functionalized photocrosslinkable liver decellularized extracellular matrix (dECM). By combining liver dECM methacrylate (LdECMMA) with gelatin methacrylate (GelMA), we achieved accelerated crosslinking under visible light irradiation and the ability to tune the mechanical, rheological, and physiological properties of the material. We encapsulated human hepatocellular carcinoma cells within an optimal concentration of the GelMA-LdECMMA hybrid hydrogel and examined cell proliferation and function over an extended period. The findings revealed that the GelMA-LdECMMA hybrid hydrogel enhances liver cell proliferation and function, holding significant promise for applications in drug screening and liver cancer metastasis research.

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Section: Printability Evaluationmentioning

confidence: 99%

3D bioprinting of Liver Microenvironment Model Using Photocrosslinkable Decellularized Extracellular Matrix based Hydrogel

Tabatabaei Rezaei,

Kumar,

Liu

et al. 2024

Preprint

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“…Pluronic-based Pluronic, modified Pluronic [22,61] Alginate [32,37,62,175,176] Gelatin or GelMA -based [32,37,103,155,176] Collagen [32,37] Polyelectrolyte complex [177] PDMS, Silicone [178] 𝛼-calcium phosphate / Pluronic P123, 𝛼-calcium phosphate / hydroxypropyl methylcellulose [179] Silicone-based Carbon conductive grease [72] Photocrosslinkable PVA [180] Water/carboxylic acid-functionalized silica nanoparticles [181] PDMS, Silicone [81,180,182,183] Xanthan gum [65,184] Pluronic [184] Hyaluronic acid (HA)-based HA, modified HA [59,60,79,185] Spheroids [186] GelMA [79] Modified HA / PEDGA / Agarose μP [ 133] Carbopol PVA [180] PDMS, Silicone [49,87,117,129,180,[187][188][189] Alginate [143,[190][191]…”

Section: Support Bath Materials Bioink Referencesmentioning

confidence: 99%

“…Alginate / GelMA, [ 176] GelMA [ 32,37,103] , Polyelectrolyte complex, [ 177] poly(acrylamide) μP [ 222] Pluronic-based Modified HA [ 59,60] Hyaluronic acid-based Modified HA [ 156,157] , GelMA/poly (3,4-ethylenedioxythiophene): poly(styrenesulfonate) , [ 146] GelMA, GelMA / collagen methacrylate, [ 148] Poly(ethylene glycol) methacrylate [ 162] Gelatin μP GelMA-based [ 45,95,205,207,208] , PEGDA/Acrylamide [143] Agarose Photocrosslinkable PVA, [ 180] GelMA-based [ 26,191,194] , Photocrosslinkable CNC, [ 200] Photocroslinkable resin, [ 221] Carbopol Photocrosslinkable silicone [ 81,182,215,224,225] , Photocroslinkable resin, [ 217] Xanthan Gum/ Pluronic [ 184] Silicone-based or mineral oil-based Cells, [ 166] Alginate/CNC/ GelMA, [ 211] GelMA [ 165,226] , PEGDA,…”

Section: Photomentioning

confidence: 99%

Dissecting the Interplay Mechanism among Process Parameters toward the Biofabrication of High‐Quality Shapes in Embedded Bioprinting

Wu,

Yang,

Gupta

et al. 2024

Adv Funct Materials

Self Cite

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Embedded bioprinting overcomes the barriers associated with the conventional extrusion‐based bioprinting process as it enables the direct deposition of bioinks in 3D inside a support bath by providing in situ self‐support for deposited bioinks during bioprinting to prevent their collapse and deformation. Embedded bioprinting improves the shape quality of bioprinted constructs made up of soft materials and low‐viscosity bioinks, leading to a promising strategy for better anatomical mimicry of tissues or organs. Herein, the interplay mechanism among the printing process parameters toward improved shape quality is critically reviewed. The impact of material properties of the support bath and bioink, printing conditions, cross–linking mechanisms, and post‐printing treatment methods, on the printing fidelity, stability, and resolution of the structures is meticulously dissected and thoroughly discussed. Further, the potential scope and applications of this technology in the fields of bioprinting and regenerative medicine are presented. Finally, outstanding challenges and opportunities of embedded bioprinting as well as its promise for fabricating functional solid organs in the future are discussed.

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Nested Biofabrication: Matryoshka‐Inspired Intra‐Embedded Bioprinting

Alioglu,

Yilmaz,

Singh

et al. 2023

Small Methods

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Engineering functional tissues and organs remains a fundamental pursuit in bio‐fabrication. However, the accurate constitution of complex shapes and internal anatomical features of specific organs, including their intricate blood vessels and nerves, remains a significant challenge. Inspired by the Matryoshka doll, here a new method called “Intra‐Embedded Bioprinting (IEB)” is introduced building upon existing embedded bioprinting methods. a xanthan gum‐based material is used which served a dual role as both a bioprintable ink and a support bath, due to its unique shear‐thinning and self‐healing properties. IEB's capabilities in organ modeling, creating a miniaturized replica of a pancreas using a photocrosslinkable silicone composite is demonstrated. Further, a head phantom and a Matryoshka doll are 3D printed, exemplifying IEB's capability to manufacture intricate, nested structures. Toward the use case of IEB and employing an innovative coupling strategy between extrusion‐based and aspiration‐assisted bioprinting, a breast tumor model that included a central channel mimicking a blood vessel, with tumor spheroids bioprinted in proximity is developed. Validation using a clinically‐available chemotherapeutic drug illustrated its efficacy in reducing the tumor volume via perfusion over time. This method opens a new way of bioprinting enabling the creation of complex‐shaped organs with internal anatomical features.

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A Versatile Photocrosslinkable Silicone Composite for 3D Printing Applications

Cited by 5 publications

References 89 publications

3D bioprinting of Liver Microenvironment Model Using Photocrosslinkable Decellularized Extracellular Matrix based Hydrogel

3D bioprinting of Liver Microenvironment Model Using Photocrosslinkable Decellularized Extracellular Matrix based Hydrogel

Dissecting the Interplay Mechanism among Process Parameters toward the Biofabrication of High‐Quality Shapes in Embedded Bioprinting

Nested Biofabrication: Matryoshka‐Inspired Intra‐Embedded Bioprinting

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