A versatile quantitation platform based on platinum nanoparticles incorporated volumetric bar-chart chip for highly sensitive assays

Wang, Yuzhen; Zhu, Guixian; Qi, Wei; Li, Ying; Song, Yujun

doi:10.1016/j.bios.2016.05.090

Cited by 36 publications

(10 citation statements)

References 39 publications

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“…Because the as-produced moving distances were correlated with the concentrations of protein biomarkers, the V-Chip achieved quantitative measurements of analytes without using costly detectors. By combining different immunoassays with V-Chips, various detection platforms have been explored thereafter to detect a broad range of biomolecular targets, leveraging the convenient analytical readouts. − Despite the promising potential of V-Chips in POC testing, there are multiple drawbacks hurdling their wide applications for POC detection and other applications. For example, the fabrication of glass microfluidic V-Chips is complicated, and requires stringent cleanroom facilities, resulting in high cost.…”

Section: Introductionmentioning

confidence: 99%

Detector-Free Photothermal Bar-Chart Microfluidic Chips (PT-Chips) for Visual Quantitative Detection of Biomarkers

Zhou

2021

Anal. Chem.

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The volumetric bar-chart microfluidic chips (V-Chips) driven by chemical reaction-generated gas provide a promising platform for point-of-care (POC) visual biomarker quantitation. However, multiple limitations are encountered in conventional V-Chips, such as costly and complex chip fabrication, complicated assembly, and imprecise controllability of gas production. Herein, we introduced nanomaterial-mediated photothermal effects to V-Chips, and for the first time developed a new type of V-Chip, photothermal bar-chart microfluidic chip (PT-Chip), for visual quantitative detection of biochemicals without any bulky and costly analytical instruments.Immunosensing signals were converted to visual readout signals via photothermal effects, the onchip bar-chart movements, enabling quantitative biomarker detection on a low-cost polymer hybrid PT-Chip with on-chip scale rulers. Four different human serum samples containing prostate-specific antigen (PSA) as a model analyte were detected simultaneously using the PT-Chip, with the limit of detection of 2.1 ng/mL, meeting clinical diagnostic requirements. Although no conventional signal detectors were used, it achieved comparable detection sensitivity to absorbance measurements with a microplate reader. The PT-Chip was further validated by testing human whole blood without the color interference problem, demonstrating good analytical performance of our method even in complex matrixes and thus the potential to fill a gap in current clinical diagnostics that is incapable of testing whole blood. This new PT-Chip driven by nanomaterial-mediated photothermal effects opens a new horizon of microfluidic platforms for instrument-free diagnostics at the point of care.

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Section: Introductionmentioning

confidence: 99%

Detector-Free Photothermal Bar-Chart Microfluidic Chips (PT-Chips) for Visual Quantitative Detection of Biomarkers

Zhou

2021

Anal. Chem.

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“…These harmonics are very useful metrics for characterizing the MNP ferrofluids such as the viscosity and temperature of the solution as well as the conjugations of chemical compounds on the MNPs. − Nowadays, with the ease of fabricating and surface-functionalizing MNPs, MNPs functionalized with ligands, DNA, RNA, and protein molecules can be readily applied as probes, labels, contrasts, and tracers for different bioassays. ,− Unlike the surface-based biosensing platforms (ELISA, GMR, Hall effect sensors, etc.) that directly detect individual target objects near the sensing elements, the volumetric-based biosensing platforms measure the analytical signals that directly come from the entire detection volume, making the bioassays simple and fast. − Representative examples of volumetric-based biosensors are the nuclear magnetic resonance (NMR) devices and the magnetic susceptometers. − MPS is one type of volumetric-based biosensing platform where the MNPs are acting as the minisensing probes, and their dynamic magnetic responses are used as metrics for the characterization of target analytes from the fluidic samples. − …”

Section: Introductionmentioning

confidence: 99%

Magnetic Particle Spectroscopy for Detection of Influenza A Virus Subtype H1N1

Liu

Saha

et al. 2020

ACS Appl. Mater. Interfaces

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Magnetic nanoparticles (MNPs) with proper surface functionalization have been extensively applied as labels for magnetic immunoassays, carriers for controlled drug/gene delivery, tracers and contrasts for magnetic imaging, etc. Here, we introduce a new biosensing scheme based on magnetic particle spectroscopy (MPS) and the self-assembly of MNPs to quantitatively detect H1N1 nucleoprotein molecules. MPS monitors the harmonics of oscillating MNPs as a metric for the freedom of rotational process, thus indicating the bound states of MNPs. These harmonics can be readily collected from nanogram quantities of iron oxide nanoparticles within 10 s. The H1N1 nucleoprotein molecule hosts multiple different epitopes that forms binding sites for many IgG polyclonal antibodies. Anchoring IgG polyclonal antibodies onto MNPs triggers the cross-linking between MNPs and H1N1 nucleoprotein molecules, thereby forming MNP self-assemblies. Using MPS and the self-assembly of MNPs, we were able to detect as low as 44 nM (4.4 pmole) H1N1 nucleoprotein. In addition, the morphologies and the hydrodynamic sizes of the MNP self-assemblies are characterized to verify the MPS results. Different MNP self-assembly models such as classical cluster, open ring tetramer, and chain model as well as multimers (from dimer to pentamer) are proposed in this paper. Herein, we claim the feasibility of using MPS and the self-assembly of MNPs as a new biosensing scheme for detecting ultralow concentrations of target biomolecules, which can be employed as rapid, sensitive, and wash-free magnetic immunoassays.

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“…This assay allows amplification and detection in approximately 35 minutes with excellent sensitivity and specificity, although viral nucleic acid extraction must initially be performed. Other novel technologies, such as portable and low-cost molecular systems [60], handheld NAAT devices [61], and the use of nanoparticles to detect isothermally amplified nucleic acids [62], have been described. Another innovative molecular assay showing promise is the feasibility of providing real-time genome sequencing in an outbreak setting [63].…”

Section: Introductionmentioning

confidence: 99%

Diagnostics in Ebola Virus Disease in Resource-Rich and Resource-Limited Settings

et al. 2016

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The Ebola virus disease (EVD) outbreak in West Africa was unprecedented in scale and location. Limited access to both diagnostic and supportive pathology assays in both resource-rich and resource-limited settings had a detrimental effect on the identification and isolation of cases as well as individual patient management. Limited access to such assays in resource-rich settings resulted in delays in differentiating EVD from other illnesses in returning travellers, in turn utilising valuable resources until a diagnosis could be made. This had a much greater impact in West Africa, where it contributed to the initial failure to contain the outbreak. This review explores diagnostic assays of use in EVD in both resource-rich and resource-limited settings, including their respective limitations, and some novel assays and approaches that may be of use in future outbreaks.

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A versatile quantitation platform based on platinum nanoparticles incorporated volumetric bar-chart chip for highly sensitive assays

Cited by 36 publications

References 39 publications

Detector-Free Photothermal Bar-Chart Microfluidic Chips (PT-Chips) for Visual Quantitative Detection of Biomarkers

Detector-Free Photothermal Bar-Chart Microfluidic Chips (PT-Chips) for Visual Quantitative Detection of Biomarkers

Magnetic Particle Spectroscopy for Detection of Influenza A Virus Subtype H1N1

Diagnostics in Ebola Virus Disease in Resource-Rich and Resource-Limited Settings

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