A versatile strategy to construct free-standing multi-furcated vessels and a complicated vascular network in heterogeneous porous scaffolds <i>via</i> combination of 3D printing and stimuli-responsive hydrogels

Su, Hongxian; Li, Qingtao; Li, Ding-Guo; Li, Haofei; Feng, Qi; Cao, Xiaodong; Dong, Hua

doi:10.1039/d2mh00314g

Cited by 36 publications

(19 citation statements)

References 73 publications

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“…In brief, a biomedical silicone tube was soaked in GelMA/HepMA hydrogel precursor solution, and ice water was injected into the tube through a syringe. Conformal hydrogel layer was condensed on the tube surface due to gelation of GelMA at low temperature [ 49 , 50 ], which was then irradiated with blue light for photopolymerization of GelMA and HepMA. The scaffold was finally acquired by immersing in VEGF solutions, where VEGF was loaded into the hydrogel tube and bound to HepMA owing to their strong biological affinity.…”

Section: Resultsmentioning

confidence: 99%

A novel bioartificial pancreas fabricated via islets microencapsulation in anti-adhesive core-shell microgels and macroencapsulation in a hydrogel scaffold prevascularized in vivo

Shang

Feng

et al. 2023

Bioactive Materials

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Section: Resultsmentioning

confidence: 99%

A novel bioartificial pancreas fabricated via islets microencapsulation in anti-adhesive core-shell microgels and macroencapsulation in a hydrogel scaffold prevascularized in vivo

Shang

Feng

et al. 2023

Bioactive Materials

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“…This chitosan−gelatin−anion system was used with 3D printing to enable the fabrication of complex hydrogel structures. 95,96 3.2. Coupling with Biomolecular Methods.…”

Section: Coupling With Other Additive Manufacturing Methodsmentioning

confidence: 99%

Electro-Biofabrication. Coupling Electrochemical and Biomolecular Methods to Create Functional Bio-Based Hydrogels

et al. 2023

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Twenty years ago, this journal published a review entitled "Biofabrication with Chitosan" based on the observations that (i) chitosan could be electrodeposited using low voltage electrical inputs (typically less than 5 V) and (ii) the enzyme tyrosinase could be used to graft proteins (via accessible tyrosine residues) to chitosan. Here, we provide a progress report on the coupling of electronic inputs with advanced biological methods for the fabrication of biopolymer-based hydrogel films. In many cases, the initial observations of chitosan's electrodeposition have been extended and generalized: mechanisms have been established for the electrodeposition of various other biological polymers (proteins and polysaccharides), and electrodeposition has been shown to allow the precise control of the hydrogel's emergent microstructure. In addition, the use of biotechnological methods to confer function has been extended from tyrosinase conjugation to the use of protein engineering to create genetically fused assembly tags (short sequences of accessible amino acid residues) that facilitate the attachment of function-conferring proteins to electrodeposited films using alternative enzymes (e.g., transglutaminase), metal chelation, and electrochemically induced oxidative mechanisms. Over these 20 years, the contributions from numerous groups have also identified exciting opportunities. First, electrochemistry provides unique capabilities to impose chemical and electrical cues that can induce assembly while controlling the emergent microstructure. Second, it is clear that the detailed mechanisms of biopolymer self-assembly (i.e., chitosan gel formation) are far more complex than anticipated, and this provides a rich opportunity both for fundamental inquiry and for the creation of high performance and sustainable material systems. Third, the mild conditions used for electrodeposition allow cells to be co-deposited for the fabrication of living materials. Finally, the applications have been expanded from biosensing and lab-on-a-chip systems to bioelectronic and medical materials. We suggest that electro-biofabrication is poised to emerge as an enabling additive manufacturing method especially suited for life science applications and to bridge communication between our biological and technological worlds.

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“…pH-responsive hydrogels could respond to the change in pH values [ 89 ]. The pH of most healthy tissues is maintained between 6.5 and 7.2.…”

Section: Biochemical Signal-responsive Hydrogels For Bone Regenerationmentioning

confidence: 99%

Smart Hydrogels for Bone Reconstruction via Modulating the Microenvironment

et al. 2023

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Rapid and effective repair of injured or diseased bone defects remains a major challenge due to shortages of implants. Smart hydrogels that respond to internal and external stimuli to achieve therapeutic actions in a spatially and temporally controlled manner have recently attracted much attention for bone therapy and regeneration. These hydrogels can be modified by introducing responsive moieties or embedding nanoparticles to increase their capacity for bone repair. Under specific stimuli, smart hydrogels can achieve variable, programmable, and controllable changes on demand to modulate the microenvironment for promoting bone healing. In this review, we highlight the advantages of smart hydrogels and summarize their materials, gelation methods, and properties. Then, we overview the recent advances in developing hydrogels that respond to biochemical signals, electromagnetic energy, and physical stimuli, including single, dual, and multiple types of stimuli, to enable physiological and pathological bone repair by modulating the microenvironment. Then, we discuss the current challenges and future perspectives regarding the clinical translation of smart hydrogels.

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A versatile strategy to construct free-standing multi-furcated vessels and a complicated vascular network in heterogeneous porous scaffolds via combination of 3D printing and stimuli-responsive hydrogels

Abstract: Mimicking complex structures of natural blood vessels and constructing vascular network in tissue engineering scaffolds are still challenging now. Herein we demonstrate a new and versatile strategy to fabricate free-standing...

Cited by 36 publications

References 73 publications

A novel bioartificial pancreas fabricated via islets microencapsulation in anti-adhesive core-shell microgels and macroencapsulation in a hydrogel scaffold prevascularized in vivo

A novel bioartificial pancreas fabricated via islets microencapsulation in anti-adhesive core-shell microgels and macroencapsulation in a hydrogel scaffold prevascularized in vivo

Electro-Biofabrication. Coupling Electrochemical and Biomolecular Methods to Create Functional Bio-Based Hydrogels

Smart Hydrogels for Bone Reconstruction via Modulating the Microenvironment

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