Large ground-based solar telescopes are equipped with adaptive optics systems to correct wavefront distortions induced in the turbulent atmosphere. The design of the adaptive optics system strongly depends on the vertical profiles of the optical turbulence. In particular, the characteristics of the optical turbulence determine the design of tomographic adaptive optics systems, which provide image correction within a wide field of view. In the article, a new method to estimate reference optical turbulence characteristics from Era-5 reanalysis assimilated data is presented. This method is based on the dependence of the air refractive index structure constant $C_n^2$ on the vertical shears of wind speed as well as the outer scale of turbulence L0. The L0 parameter is estimated by minimization of the dispersion between the modeled and measured values of the refractive index structure constant $C_n^2$ within the surface layer. For the first time, parametrization coefficients and reference profiles of optical turbulence averaged for the period 1940–2022 are calculated for the Large Solar Vacuum Telescope (LSVT) site. The calculated optical turbulence profiles are representative; these profiles correspond to typical changes of the measured values of the Fried parameter, the isoplanatic angle, and the outer scale of turbulence at the LSVT site. The model turbulence profiles are verified taking into account the Shack–Hartmann wavefront sensor measurements at the LSVT. The higher accuracy of estimation of the optical turbulence characteristics makes it possible to refine parameters relevant to the LSVT adaptive optics system. The obtained results can be used in order to develop high-resolution solar adaptive optics technologies as applied to ground-based telescopes including those using the principles of atmospheric tomography.