Abstract. In order to avoid a problem remains with the low accuracy and poor portability of the photometry system, the system based on the photometry method and microfluidic chip technology was built. As the characteristics of cheap, solid and good transmission, Polydimethylsiloxane (PDMS) was chosen as the material of the designed chip in the paper. To the designed UV photometry system, the light-emitting diode with the wavelength of 580m is chosen as the light source. The experimental result indicates that there is no significant deviation between the designed UV photometry system and the conventional immuneturbidimetric assay, the correlation coefficient is 0.95 obtained by adopting the linear regression analysis. The linearity of the designed UV photometry system based on the PDMS-based microfluidic chip has increased by 17.3% in comparison with the system based on the silicon-based microfluidic chip.