A very simple high‐performance liquid chromatographic method with fluorescence detection for the determination of gemifloxacin in human breast milk

Sağırlı, Olcay; Demirci, Seda; Önal, Armağan

doi:10.1002/bio.2901

Cited by 8 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Water was purified by an aquaMAXTMultra system (Younglin Instruments, Anyang, Korea). In the literature there are several methods for the quantitation of GMX in human plasma [5][6][7][8][9] and one method in breast milk [10]. In order to separate the anayte from proteins, lipids and other matrix constituents solid-phase extraction (SPE), liquid-liquid extraction (LLE), protein precipitation techniques were used in these studies.…”

Section: Apparatusmentioning

confidence: 99%

Magnetic Solid Phase Extraction for a New HPLC Method for the Determination of Gemifloxacin in Human Plasma and Breast Milk

Durmuș

Tekkeli

Kızıltaş

et al. 2017

J. Chil. Chem. Soc.

View full text Add to dashboard Cite

A simple analytical procedure, magnetic solid phase extraction combination to HPLC, has been developed for the analysis of gemifloxacin in human plasma and breast milk. Before chromatographic separation, magnetic solid phase extraction has been applied for sample preparation which is currently preffered extraction technique accordingly its simple, fast and efficient procedure. Fe 3 O 4 magnetic nanoparticles have been used as magnetic adsorbents, the adsorption process has been optimized. RP C18 column has been used with mobile phase composed of acetonitrile-10 mM orthophosphoric acid including 1 mL/L triethylamine (60:40) by isocratic elution with flow rate of 1.3 mL/min. Calibration curve is linear over the range of 0.5-30 µg/mL and 0.5-20 µg/mL for plasma and breast milk, respectively. LOD and LOQ has been found to be 0.15 and 0.5µg/mL for both matrices. Intraday and interday RSD values are less than 3.57% for both assays. Moreover, the newly developed method provides fast, simple, cost reduced and sensitive assay for gemifloxacin.

show abstract

Section: Apparatusmentioning

confidence: 99%

Magnetic Solid Phase Extraction for a New HPLC Method for the Determination of Gemifloxacin in Human Plasma and Breast Milk

Durmuș

Tekkeli

Kızıltaş

et al. 2017

J. Chil. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…According to a literature review, analytical methods used for the determination of gemifloxacin include spectrophotometry [ 6 , 7 , 8 ], spectrofluorometry [ 9 , 10 ], polarography [ 11 ], voltammetry [ 12 , 13 , 14 ], HP-TLC [ 15 , 16 ], HPLC-UV [ 17 , 18 , 19 ] , HPLC-F [ 20 , 21 , 22 ], HPLC-MS [ 23 , 24 , 25 ], and potentiometry [ 26 , 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Applications of Potentiometric Membrane Sensors Based on Specific Recognition Sites for the Measurement of the Quinolone Antibacterial Drug Gemifloxacin

2023

View full text Add to dashboard Cite

Supramolecular gemifloxacin (GF) sensors have been developed. Supramolecular chemistry is primarily concerned with noncovalent intermolecular and intramolecular interactions, which are far weaker than covalent connections, but they can be exploited to develop sensors with remarkable affinity for a target analyte. In order to determine the dose form of the quinolone antibacterial drug gemifloxacin, the current study’s goal is to adapt three polyvinylchloride (PVC) membrane sensors into an electrochemical technique. Three new potentiometric membrane sensors with cylindric form and responsive to gemifloxacin (GF) were developed. The sensors’ setup is based on the usage of o-nitrophenyl octyl ether (o-NPOE) as a plasticizer in a PVC matrix, β-cyclodextrin (β-CD) (sensor 1), γ-cyclodextrin (γ-CD) (sensor 2), and 4-tert-butylcalix[8]arene (calixarene) (sensor 3) as an ionophore, potassium tetrakis (4-chlorophenyl) borate (KTpClPB) as an ion additive for determination of GF. The developed method was verified according to IUPAC guidelines. The sensors under examination have good selectivity for GF, according to their selectivity coefficients. The constructed sensors demonstrated a significant response towards to GF over a concentration range of 2.4 × 10−6, 2.7 × 10−6, and 2.42 × 10−6 mol L−1 for sensors 1, 2, and 3, respectively. The sensors showed near-Nernstian cationic response for GF at 55 mV, 56 mV, and 60 mV per decade for sensors 1, 2, and 3, respectively. Good recovery and relative standard deviations during the day and between days are displayed by the sensors. They demonstrated good stability, quick response times, long lives, rapid recovery, and precision while also exhibiting good selectivity for GF in various matrices. To determine GF in bulk and dose form, the developed sensors have been successfully deployed. The sensors were also employed as end-point indicators for titrating GF with sodium tetraphenyl borate.

show abstract

“…Electrochemical methods were also reported for the determination of quinolones [13,14]. The reported HPTLC methods included the determination of MX in tablets [15], MX with other fluoroquinolone antibiotics in industrial wastewater [16], LV with ornidazole or cefpodoxime [17,18] and GM [19,20]; the reported HPLC methods involved the determination of MX [21][22][23], LV [24,25], MX by stability studies [26], LV in urine [27], and GM in pharmaceutical and biological matrices [28][29][30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Two Eco-Friendly Chromatographic Methods Evaluated by GAPI for Simultaneous Determination of the Fluoroquinolones Moxifloxacin, Levofloxacin, and Gemifloxacin in Their Pharmaceutical Products

et al. 2022

View full text Add to dashboard Cite

In this paper, novel green HPLC and HPTLC chromatographic methods were developed for the concurrent determination of moxifloxacin, levofloxacin, and gemifloxacin in bulk and pharmaceutical products. The green HPLC method was used on Thermo C18 (4.6 × 250 mm, 5 µm). By mixing ethanol and 20 mM sodium dihydrogen phosphate dihydrate (pH 5) in a ratio of 25:75, v/v, the mobile phase was created using isocratic elution. The flow rate was 1 mLmin−1. The studied antibiotics were separated well within 9.5 min. The green HPTLC method was used on coated HPTLC aluminum sheets with Silica gel 60 F254 using a mobile phase mixture of water: acetone: ammonia (8:1:1, v/v/v). Compact and well-resolved peaks were obtained under chamber-saturation circumstances for the standard fluoroquinolone antibiotics. Both methods were optimized individually, validated by ICH, and assessed using the Green analytical procedure index (GAPI). The methods were applied to pharmaceutical products and compared with the published methods for the determination of each of these antibiotics individually, using Student’s t-test. They can be used by quality-control laboratories in pharmaceutical factories as sensitive eco-friendly methods for the analysis of these drugs and for the detection of cross-contamination during manufacturing processes.

show abstract

A very simple high‐performance liquid chromatographic method with fluorescence detection for the determination of gemifloxacin in human breast milk

Cited by 8 publications

References 11 publications

Magnetic Solid Phase Extraction for a New HPLC Method for the Determination of Gemifloxacin in Human Plasma and Breast Milk

Magnetic Solid Phase Extraction for a New HPLC Method for the Determination of Gemifloxacin in Human Plasma and Breast Milk

Fabrication and Applications of Potentiometric Membrane Sensors Based on Specific Recognition Sites for the Measurement of the Quinolone Antibacterial Drug Gemifloxacin

Two Eco-Friendly Chromatographic Methods Evaluated by GAPI for Simultaneous Determination of the Fluoroquinolones Moxifloxacin, Levofloxacin, and Gemifloxacin in Their Pharmaceutical Products

Contact Info

Product

Resources

About