Temperature Sensitive Electrical Parameters (TSEP) provide an indirect and non-invasive method for online junction temperature estimation of power semiconductor devices. It is known that the fundamental of TSEP-based methods is to calibrate the relationship between TSEP and junction temperature in advance. However, the calibration methods in the literature need to open the module, require pre-testing, or record the operating data of a converter in the entire power rating range, which are inconvenient in field applications. This paper proposes an on-line and non-invasive calibration method by measuring the data at three operating states that are already existed in the regular converter operation. It is achieved by measuring the accessible heatsink/case temperature and TSEP during converter regular operation. The concept, implementation, and error analysis of the proposed method are presented in this paper. Experimental verification is given to prove the effectiveness, accuracy, and convenience of the proposed method.