A Virulence and Antimicrobial Resistance DNA Microarray Detects a High Frequency of Virulence Genes in
            <i>Escherichia coli</i>
            Isolates from Great Lakes Recreational Waters

Hamelin, Katia; Bruant, Guillaume; El‐Shaarawi, A. H.; Hill, Stephen J.; Edge, Thomas A.; Békal, Sadjia; Fairbrother, John M.; Harel, Josée; Maynard, Christine; Masson, Luke; Brousseau, Roland

doi:10.1128/aem.00137-06

Cited by 104 publications

(115 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…coli pathotypes such as STEC, EPEC, ETEC, and ExPEC and their associated VGs were selected in this study, as other studies have reported the presence of these pathotypes, originating from sewage and/or animal feces, in environmental water (23,24,25,38,46). Overall, 30 (15%) of 200 isolates from eight tanks were positive for the eaeA gene, which belongs to STEC or EPEC (25).…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Intestinal and Extraintestinal Virulence Genes in Escherichia coli Isolates from Rainwater Tanks in Southeast Queensland, Australia

Ahmed

Hodgers

Masters

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

In this study, 200 Escherichia coli isolates from 22 rainwater tank samples in Southeast Queensland, Australia, were tested for the presence of 20 virulence genes (VGs) associated with intestinal and extraintestinal pathotypes. In addition, E. coli isolates were also classified into phylogenetic groups based on the detection of the chuA, yjaA, and TSPE4.C2 genes. Of the 22 rainwater tanks, 8 (36%) and 5 (23%) were positive for the eaeA (belonging to enteropathogenic E. coli [EPEC] and Shiga-toxigenic E. coli [STEC]) and ST1 (belonging to enterotoxigenic E. coli [ETEC]) genes, respectively. VGs (cdtB, cvaC, ibeA, kpsMT allele III, PAI, papAH, and traT) belonging to extraintestinal pathogenic E. coli (ExPEC) were detected in 15 (68%) of the 22 rainwater tanks. Of the 22 samples, 17 (77%) and 11 (50%) contained E. coli belonging to phylogenetic groups A and B1, respectively. Similarly, 10 (45%) and 16 (72%) contained E. coli belonging to phylogenetic groups B2 and D, respectively. Of the 96 of the 200 strains from 22 tanks that were VG positive, 40 (42%) were carrying a single VG, 36 (37.5%) were carrying two VGs, 17 (18%) were carrying three VGs, and 3 (3%) had four or more VGs. This study reports the presence of multiple VGs in E. coli strains belonging to the STEC, EPEC, ETEC, and ExPEC pathotypes in rainwater tanks. The public health risks associated with potentially clinically significant E. coli in rainwater tanks should be assessed, as the water is used for drinking and other, nonpotable purposes. It is recommended that rainwater be disinfected using effective treatment procedures such as filtration, UV disinfection, or simply boiling prior to drinking.

show abstract

Section: Discussionmentioning

confidence: 99%

Occurrence of Intestinal and Extraintestinal Virulence Genes in Escherichia coli Isolates from Rainwater Tanks in Southeast Queensland, Australia

Ahmed

Hodgers

Masters

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Prehybridization and hybridizations were performed as previously described (19), with the following modifications: hybridizations were performed using a Slide Booster hybridization work station (model SB800; Advalytix, Germany), and scanning was performed using a ScanArray Lite fluorescent microarray analysis system (Perkin-Elmer, Mississauga, ON, Canada) with images scanned at 5-m resolution and laser power set to 95%. Acquisition of fluorescent spots and quantification of fluorescent spot intensities were performed as described by Hamelin et al (19).…”

Section: Methodsmentioning

confidence: 99%

“…To this end, an E. coli DNA virulence microarray previously employed to assess the genotypes (virulence and antibiotic resistance genes) of E. coli strains isolated from different environmental ecosystems and from the chicken intestinal tract (1,10,19,20,33) was used. The aim of the present trial was to investigate the distributions of pathotypes and of virulence and antibiotic resistance genes in E. coli isolates from broilers fed with antimicrobial supplementation diets including bambermycin, penicillin, salinomycin, bacitracin, chlortetracycline, virginiamycin, monensin, and narasin.…”

mentioning

confidence: 99%

Pathotype and Antibiotic Resistance Gene Distributions of Escherichia coli Isolates from Broiler Chickens Raised on Antimicrobial-Supplemented Diets

Bonnet

Diarrassouba

Brousseau

et al. 2009

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

The impact of feed supplementation with bambermycin, monensin, narasin, virginiamycin, chlortetracycline, penicillin, salinomycin, and bacitracin on the distribution of Escherichia coli pathotypes in broiler chickens was investigated using an E. coli virulence DNA microarray. Among 256 E. coli isolates examined, 59 (23%) were classified as potentially extraintestinal pathogenic E. coli (ExPEC), while 197 (77%) were considered commensal. Except for chlortetracycline treatment, the pathotype distribution was not significantly different among treatments (P > 0.05). Within the 59 ExPEC isolates, 44 (75%) were determined to be potentially avian pathogenic E. coli (APEC), with the remaining 15 (25%) considered potentially "other" ExPEC isolates. The distribution within phylogenetic groups showed that 52 (88%) of the ExPEC isolates belonged to groups B2 and D, with the majority of APEC isolates classified as group D and most commensal isolates (170, 86%) as group A or B1. Indirect assessment of the presence of the virulence plasmid pAPEC-O2-ColV showed a strong association of the plasmid with APEC isolates. Among the 256 isolates, 224 (88%) possessed at least one antimicrobial resistance gene, with nearly half (107, 42%) showing multiple resistance genes. The majority of resistance genes were distributed among commensal isolates. Considering that the simultaneous detection of antimicrobial resistance tet(A), sulI, and bla TEM genes and the integron class I indicated a potential presence of the resistance pAPEC-O2-R plasmid, the results revealed that 35 (14%) of the isolates, all commensals, possessed this multigene resistance plasmid. The virulence plasmid was never found in combination with the antimicrobial resistance plasmid. The presence of the ColV plasmid or the combination of iss and tsh genes in the majority of APEC isolates supports the notion that when found together, the plasmid, iss, and tsh serve as good markers for APEC. These data indicate that different resistant E. coli pathotypes can be found in broiler chickens and that the distribution of such pathotypes and certain virulence determinants could be modulated by antimicrobial agent feed supplementation.

show abstract

“…The microarray used in this study is based on earlier published work , which contains 312 oligonucleotides (70 bases in length) targeting 183 virulence or virulence-related genes and 30 of the most common antimicrobial resistance genes found in Gramnegative bacteria (Hamelin et al 2006). …”

Section: Dna Microarraymentioning

confidence: 99%

“…The tube was re-centrifuged as before and 150 mL of the supernatant containing DNA was removed and stored at (208C until used. Thawed lysate DNA was linearly amplified and labeled using the Bioprime † DNA Labeling System (Invitrogen Life Technologies, Burlington, ON) as described previously (Hamelin et al 2006). Briefly, 4 mL of RNase-treated supernatant were added to a final volume of 50 mL containing 20 mL of a random primer solution, 1 mL of persistent concentration DNA polymerase (Klenow fragment, 40 U mL…”

Section: Intestinal Tissuementioning

confidence: 99%

Escherichia coli O157:H7-secreted cytotoxins are toxic to enterocytes and increase Escherichia coli O157:H7 colonization of jejunum and descending colon in cattle

Baines¹,

Masson²,

McAllister³

2008

Can. J. Anim. Sci.

Self Cite

View full text Add to dashboard Cite

, T. 2008. Escherichia coli O157:H7-secreted cytotoxins are toxic to enterocytes and increase Escherichia coli O157:H7 colonization of jejunum and descending colon in cattle. Can. J. Anim. Sci. 88: 41Á50. Enterohemorrhagic Escherichia coli (EHEC) O157:H7-secreted cytotoxins are toxic to target cells and enhance colonization of intestinal tissues in disease-susceptible animals. It is unclear what role, if any, EHEC O157:H7-secreted cytotoxins play in the colonization of intestinal tissues of healthy reservoir animals. We previously reported that EHEC O157:H7 colonization sites were associated with focal hemorrhages in the jejunum and descending colon of persistent shedding cattle, suggesting a potential role for cytotoxins in EHEC O157:H7 colonization. We have used a traditional EHEC O157:H7 IVOC adherence assay and a novel lawn assay to examine the role of EHEC O157:H7-secreted cytotoxins in EHEC O157:H7 strain colonization of the jejunum and descending colon of non-persistent and persistent shedding cattle. Four EHEC O157:H7 strains that were previously reported to differentially colonize cattle produced cytotoxins that were differentially active against epithelial cells from the jejunum and descending colon. There was a relationship between EHEC O157:H7-secreted cytotoxin activity and strain adherence for epithelial cells from the jejunum and descending colon of cattle. There was also a greater susceptibility of epithelial cells from the jejunum and descending colon to EHEC O157:H7-secreted cytotoxins of persistent shedding cattle compared with non-persistent shedding cattle. Addition of the most active secreted cytotoxins from EHEC O157:H7 R318N to the IVOC adherence assays significantly increased the adherence of the most (R318N) and least (H4420N) virulent EHEC O157:H7 strain to intestinal tissues. The current study supports a role for EHEC O157:H7-secreted cytotoxins in enhancing EHEC O157:H7 colonization of intestinal tissues of cattle.Key words: Escherichia coli O157:H7, cattle, intestine, cytotoxins, colonization Baines, D., Masson, L. et McAllister, T. 2008. Les cytotoxines se´cre´te´es par Escherichia coli O157:H7 sont toxiques pour les ente´rocytes et favorisent la colonisation du je´junum et du coˆlon descendant des bovins par le microorganisme. Can. J. Anim. Sci. 88: 41Á50. Les cytotoxynes se´cre´te´es par la souche O157:H7 d'Escherichia coli ente´ro-he´morragique (EHEC) sont toxiques pour les cellules cibles et favorisent la colonisation des tissus intestinaux chez les animaux sensibles a`la maladie. Le roˆle e´ventuel des cytotoxynes de EHEC O157:H7 dans la colonisation des tissus intestinaux des porteurs sains n'est toutefois pas claire. Ante´rieurement, les auteurs avaient signale´que les sites colonise´s par EHEC O157:H7 e´taient associe´s a`des foyers d'he´morragie dans le je´junum et le coˆlon descendant des animaux excre´teurs, signe que les cytotoxynes libe´re´es par le microorganisme pourraient intervenir. Ils ont recouru a`une e´preuve classique d'adhe´rence IVOC pour EHEC O157:H7 ainsi ...

show abstract

A Virulence and Antimicrobial Resistance DNA Microarray Detects a High Frequency of Virulence Genes in Escherichia coli Isolates from Great Lakes Recreational Waters

Cited by 104 publications

References 38 publications

Occurrence of Intestinal and Extraintestinal Virulence Genes in Escherichia coli Isolates from Rainwater Tanks in Southeast Queensland, Australia

Occurrence of Intestinal and Extraintestinal Virulence Genes in Escherichia coli Isolates from Rainwater Tanks in Southeast Queensland, Australia

Pathotype and Antibiotic Resistance Gene Distributions of Escherichia coli Isolates from Broiler Chickens Raised on Antimicrobial-Supplemented Diets

Escherichia coli O157:H7-secreted cytotoxins are toxic to enterocytes and increase Escherichia coli O157:H7 colonization of jejunum and descending colon in cattle

Contact Info

Product

Resources

About