A virus related to Soybean mosaic virus from Pinellia ternata in China and its comparison with local soybean SMV isolates

Abstract

A potyvirus isolated from Pinellia ternata in China was characterised and shown to be related to Soybean mosaic virus (SMV). The virus was pathogenic on P. ternata and some soybean cultivars, whereas the local soybean SMV isolate HH5 did not infect P. ternata. Western blot experiments demonstrated a serological relationship between the virus from Pinellia, SMV and Watermelon mosaic virus (WMV). The complete nucleotide sequences of the Pinellia virus (isolate P-1, 9735 nt) and of the Chinese soybean SMV isolates HH5 (9585 nt) and HZ (9588 nt) were determined. A 1733 nt sequence at the 3'-terminus of a second isolate from Pinellia (isolate P-2) was also determined. The predicted polyprotein of isolate P-1 has 83% amino acid (aa) identity with those of published SMV sequences. In many parts of the genome, aa identity was about 90% but it was much lower in the P1 protein region (24-29%), where it more closely resembled Dasheen mosaic virus (62%). The partial sequence of isolate P-2 had 91% nt identity to P-1 and both isolates resembled a recent sequence in the public databases (AF469171) wrongly named Zantedeschia mosaic virus. The two complete SMV soybean sequences had 93-95% nt identity with those of the previously sequenced isolates and >97% amino acid identity. Phylogenetic analysis and comparisons of coat proteins suggest that the Pinellia, WMV and SMV potyviruses should probably be treated as strains of the same species.

Molecular Characterization of Soybean Mosaic Virus NIa Protein and its Processing Event in Bacterial Expression

“…AJ619757. For sequence comparisons, NIa gene products of the reference isolates were obtained from the NCBI data library including SMV-G2 (S42280) and -G7-1 (AF241739; then designated G7 in USA) [26] , SMV-G7d (AY216987) [27] , SMV-N (NC002634) [28] , SMV-G5b (AY294044: then designated G5 in Korea) and -G7H (AY294045) [29] , SMV-HH5 (AJ310200: then designated Huanghuai) and -HZ (AJ312439: then designated Severe) [30] , as well as web-published isolates SMV-G72 (AY216010), -Aa (AB100442) and -Aa15-M2 (AB100443). Distance matrices for complete NIa sequences were calculated from the multiple sequence alignments by the DNAMAN version 5.2.9 (Lynnon Biosoft, Quebec, Canada).…”

Variability of Fungal Rhizosphere and Its Role as Antagonist Agents of Fusarium oxysporum Causing Wilt Disease in Crysanthenum sp.

“…Gen CP SMV memiliki homologi yang sangat dekat dengan BCMV, namun terpisah jauh dengan Bean common mosaic necrosis virus (BCMNV) (Chen et al 2003). Reaksi silang antara SMV dan BCMV diduga terjadi juga dalam penelitian ini.…”

The complete genomic sequence of Tobacco vein banding mosaic virus and its similarities with other potyviruses

“…Subsequently, S primer (5¢-GGN AAY AAY AGY GGN CAR CC-3¢) and the primer mentioned above were used to amplify the first fragment. Primers pCI(+) (5¢-GTN GGN TCN GGN AAN TCN AC-3¢) and pP3new(+) (5¢-CTN NTN TGY GAY AAY CRR YTN GA-3¢) [10] were used sequentially in PCR together with primers designed according to newly determined sequences to amplify most of the viral genomic sequence. PCR reactions were carried out using an Expand TM Long Template PCR system (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer's protocols.…”