A virus-specific thymidine kinase in BHK21 cells infected with herpes simplex virus

Klemperer, H. G.; Haynes, GR; Shedden, W. I. H.; Watson, Derek

doi:10.1016/0042-6822(67)90015-3

Cited by 170 publications

(74 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Involvement of thymidine kinase activity during infection of cells with DNA viruses and DNA tumor viruses (25)(26)(27) and during induction with IdUrd of the Epstein-Barr virus in Burkitt lymphoblastoid cells (28,29) has previously been shown.…”

Section: Inhibition Of Virusmentioning

confidence: 99%

Induction of Endogenous Virus and of Thymidine Kinase by Bromodeoxyuridine in Cell Cultures Transformed by Friend Virus

Ostertag

Roesler

Krieg

et al. 1974

Proc. Natl. Acad. Sci. U.S.A.

106

View full text Add to dashboard Cite

Thymidine kinase positive (TK+) N type cell lines that had been transformed by spleen focus-forming virus were established by transformation with NB tropic Friend virus complex. Thymidine kinase deficient (TK-) cell clones were isolated. Some of these cell clones release 1000-to 100,000-fold reduced amounts of Friend virus complex as compared to the TK+ parental cell clone. TK-clones were grown in medium without BrdUrd. Some of these TK-clones can be induced to release endogenous helper virus and transforming spleen focus-forming virus on reexposure to 10-10-4 M BrdUrd. BrdUrd inhibits the replication of C-type tumor viruses (1), whereas in some instances BrdUrd or IdUrd can induce endogenous C-type virus (2-4). In order to study the action of BrdUrd on virus replication and its role in the induction of endogenous virus we have used Friend virus (FV) infected and transformed DBA-2 mouse spleen cells in culture. The FV complex consists of two components, the lymphatic leukemia helper virus (LLV-F) and the replication-defective erythroid cell-transforming spleen focus-forming virus (SFFV-F) (5, 6). The RNA of the two virus types is presumably different in size and structure (7). We have established various cell lines transformed by the SFFV-F (8, 9) which, after another thymidine analogue, can be phosphorylated to azidothymidine triphosphate. However, the presence of an No group at the 3' terminal of the deoxysugar interferes with esterification, so presumably azidothymidine can only be added terminally to the growing DNA strand. Both azidothymidine as well as BrdUrd can be used to decrease the virus titer of BrdUrd-sensitive erythroleukemic cells. We have isolated several BrdUrd-resistant clones by treatment of sensitive cells with high doses of BrdUrd (11) and have shown that some of these clones have either decreased or barely detectable thymidine kinase (ATP: thymidine 5'-phosphotransferase, EC 2.7.1.75) activity (11). Furthermore, they have a 108-to 104-fold decreased virus titer. The decrease in C-type virus release can also be shown by electron microscopy.A temporary thymidine kinase activation is observed if BrdUrd is added to some virus negative lines. The same cells release large numbers of endogenous C-type and spleen focusforming virus on exposure to BrdUrd but not to azidothymidine. The RNA tumor virus which is induced by BrdUrd has mainly N-type host range properties unlike the original NB type FV complex which has been used for the transformation of our erythroid cell lines. Thymidine kinase induction and virus induction are always correlated. We have independent evidence (Ostertag, Crozier, and Swetly, unpublished)

show abstract

Section: Inhibition Of Virusmentioning

confidence: 99%

Induction of Endogenous Virus and of Thymidine Kinase by Bromodeoxyuridine in Cell Cultures Transformed by Friend Virus

Ostertag

Roesler

Krieg

et al. 1974

Proc. Natl. Acad. Sci. U.S.A.

106

View full text Add to dashboard Cite

show abstract

“…The assay was based on that for herpes simplex virus-stimulated thymidine kinase activity described by Klemperer et al (1967). The optimum conditions used to assay the virus-induced enzyme and uninfected cell enzyme activities were as follows.…”

Section: Virus and Cells Spodoptera Frugiperda Cells (Vaughnmentioning

confidence: 99%

Baculovirus Replication: Stimulation of Thymidine Kinase and DNA Polymerase Activities in Spodoptera frugiperda Cells Infected with Trichoplusia ni Nuclear Polyhedrosis Virus

Kelly

1981

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…t Extracts for TK and DNA polymerase assays were prepared from TK-BHK cells (BU-BHK) as described previously (Larder & Darby, 1982). TK activity was measured using the method of Klemperer et al (1967), with…”

mentioning

confidence: 99%

Restoration of Wild-type Pathogenicity to an Attenuated DNA Polymerase Mutant of Herpes Simplex Virus Type 1

Larder¹,

Lisle

Darby³

1986

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYThe drug-resistant variant, RSC-26, which was derived from the herpes simplex virus type 1 wild-type strain SC16, expresses an altered DNA polymerase and has reduced pathogenicity in animal models. To determine whether the attenuation in pathogenicity was due solely to mutation in the polymerase gene, a fragment of the wild-type gene was cloned, transferred into the genome of RSC-26 and recombinants were isolated. Three recombinants examined had similar properties to wild-type virus with respect to their sensitivity to antiviral drugs, DNA polymerase activities and their pathogenicity for mice. These results strongly suggest that expression of the altered polymerase of RSC-26 results in attenuated pathogenicity.

show abstract

A virus-specific thymidine kinase in BHK21 cells infected with herpes simplex virus

Cited by 170 publications

References 28 publications

Induction of Endogenous Virus and of Thymidine Kinase by Bromodeoxyuridine in Cell Cultures Transformed by Friend Virus

Induction of Endogenous Virus and of Thymidine Kinase by Bromodeoxyuridine in Cell Cultures Transformed by Friend Virus

Baculovirus Replication: Stimulation of Thymidine Kinase and DNA Polymerase Activities in Spodoptera frugiperda Cells Infected with Trichoplusia ni Nuclear Polyhedrosis Virus

Restoration of Wild-type Pathogenicity to an Attenuated DNA Polymerase Mutant of Herpes Simplex Virus Type 1

Contact Info

Product

Resources

About