A vision-based hybrid particle tracking velocimetry (PTV) technique using a modified cascade correlation peak-finding method

Lei, Y.-C.; Tien, Wei-Hsin; Duncan, James S.; Paul, Manoranjan; Ponchaut, Nicolas F.; Mouton, Christopher A.; Dabiri, Dana; Rösgen, Thomas; Hove, Jay R.

doi:10.1007/s00348-012-1357-6

Cited by 31 publications

(37 citation statements)

References 36 publications

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“…Mickoleit et al and our laboratory have developed a rigorous method for reconstruction of zebrafish RBC movement in 3D with a high spatiotemporal system (35); however, modeling the circulating RBCs remains unresolved. Further optimization of the 4D light-sheet imaging methods may allow improved 4D volume-mapping techniques in future work (47).…”

Section: Discussionmentioning

confidence: 99%

Spatial and temporal variations in hemodynamic forces initiate cardiac trabeculation

Lee¹,

Vedula²,

Baek³

et al. 2018

JCI Insight

112

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Hemodynamic shear force has been implicated as modulating Notch signaling-mediated cardiac trabeculation. Whether the spatiotemporal variations in wall shear stress (WSS) coordinate the initiation of trabeculation to influence ventricular contractile function remains unknown. Using light-sheet fluorescent microscopy, we reconstructed the 4D moving domain and applied computational fluid dynamics to quantify 4D WSS along the trabecular ridges and in the groves. In WT zebrafish, pulsatile shear stress developed along the trabecular ridges, with prominent endocardial Notch activity at 3 days after fertilization (dpf), and oscillatory shear stress developed in the trabecular grooves, with epicardial Notch activity at 4 dpf. Genetic manipulations were performed to reduce hematopoiesis and inhibit atrial contraction to lower WSS in synchrony with attenuation of oscillatory shear index (OSI) during ventricular development. γ-Secretase inhibitor of Notch intracellular domain (NICD) abrogated endocardial and epicardial Notch activity. Rescue with NICD mRNA restored Notch activity sequentially from the endocardium to trabecular grooves, which was corroborated by observed Notch-mediated cardiomyocyte proliferations on WT zebrafish trabeculae. We also demonstrated in vitro that a high OSI value correlated with upregulated endothelial Notch-related mRNA expression. In silico computation of energy dissipation further supports the role of trabeculation to preserve ventricular structure and contractile function. Thus, spatiotemporal variations in WSS coordinate trabecular organization for ventricular contractile function.

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Section: Discussionmentioning

confidence: 99%

Spatial and temporal variations in hemodynamic forces initiate cardiac trabeculation

Lee¹,

Vedula²,

Baek³

et al. 2018

JCI Insight

112

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“…Typically, the boundaries of the particle images are defined to be at the radial location at which the intensity has decreased to e -2 of the center value. Thus, the critical area is A crit = p D 2 , since particle images share the same boundary if the centers have a distance of D. Lei et al (2012) have shown that the detection of the particle image center is still possible even when the particle image overlap reaches 50 % (L = D/2), which implies that the critical area reduces to A crit = p L 2 , with L being the distance of particle image centers that can be separated. Figure 1 illustrates the ratio of overlapping particle images as a function of L for different particle image densities N ppp .…”

Section: Particle Image Positioningmentioning

confidence: 99%

“…These images have been used by many other researchers, and a detailed analysis and comprehensive collection of the results throughout the literature can be found in Lei et al (2012). Ohmi and Li (2000) applied a particle image identification algorithm first and were able to detect approximately 1,000 to 1,300 particles images out of the total 4,000 particle images per frame.…”

Section: Vsj Standard Piv Imagesmentioning

confidence: 99%

“…Unfortunately, this data are not provided by Ohmi and Li (2000). However, for modern algorithms, 98 % (Brevis et al 2011) and 97 % (Lei et al 2012) of the possible vectors could be resolved correctly using two successive frames.…”

Section: Vsj Standard Piv Imagesmentioning

confidence: 99%

“…3 that in the case of a uniform flow, nearly every detected vector represents the right velocity (R 2 & 1). At these high particle image densities, the final limitation of the particle tracking is not any longer the tracking algorithm but the ability to determine the particles' centers reliably (Lei et al 2012). …”

Section: Monte-carlo Simulationmentioning

confidence: 99%

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Higher order multi-frame particle tracking velocimetry

2013

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Particle tracking velocimetry methods (PTV) have a great potential to enhance the spatial resolution compared to spatial correlation-based methods (PIV). In addition, they are not biased due to inhomogeneous seeding concentration or in-plane and out-of-plane gradients so that the measurement precision can be increased as well. The possibility to simultaneously measure the velocity with the temperature, ph-value, or pressure of the flow at the particle location by means of fluorescent particles is another advantage of PTV. However, at high seeding concentrations, the reliable particle pairing is challenging, and the measurement precision decreases rapidly due to overlapping particle images and wrong particle image pairing. In this paper, it is shown that the particle image information acquired at four or more time steps greatly enhances a reliable particle pairing even at high seeding concentrations. Furthermore, it is shown that the accuracy and precision can be increased by using vector reallocation and displacement estimation using a fit of the trajectory in the case of curved particle paths. The improvements increase the PTV working range as reliable and accurate measurements become possible at seeding concentrations typically used for PIV measurements.

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