A visual detection of bisphenol A based on peroxidase-like activity of hemin–graphene composites and aptamer

Xiong, Zhengwei; Zhong, Haixia; Zheng, Shuzhan; Deng, Pengxi; Li, Ning; Yun, Wen; Yang, Lizhu

doi:10.1039/c8ay00677f

Cited by 19 publications

(2 citation statements)

References 41 publications

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“…Under optimized conditions, the LOD reached 40 nM. Similar methods were reported to detect bisphenol A using hemin-rGO nanozymes [ 92 ], as well as Hg(II) and Pb (II) using the peroxidase-like activity of GO–AuNP nanohybrids [ 93 ]. Moreover, RNA aptamers were also found preventing the hexadecyltrimethyl ammonium bromide (CTAB)-induced aggregation of AuNPs and improving the peroxidase-like activity of AuNPs.…”

Section: Nanozyme-based Aptasensorsmentioning

confidence: 70%

Regulation Mechanism of ssDNA Aptamer in Nanozymes and Application of Nanozyme-Based Aptasensors in Food Safety

Wang

Zhou

et al. 2022

Foods

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Food safety issues are a worldwide concern. Pathogens, toxins, pesticides, veterinary drugs, heavy metals, and illegal additives are frequently reported to contaminate food and pose a serious threat to human health. Conventional detection methods have difficulties fulfilling the requirements for food development in a modern society. Therefore, novel rapid detection methods are urgently needed for on-site and rapid screening of massive food samples. Due to the extraordinary properties of nanozymes and aptamers, biosensors composed of both of them provide considerable advantages in analytical performances, including sensitivity, specificity, repeatability, and accuracy. They are considered a promising complementary detection method on top of conventional ones for the rapid and accurate detection of food contaminants. In recent years, we have witnessed a flourishing of analytical strategies based on aptamers and nanozymes for the detection of food contaminants, especially novel detection models based on the regulation by single-stranded DNA (ssDNA) of nanozyme activity. However, the applications of nanozyme-based aptasensors in food safety are seldom reviewed. Thus, this paper aims to provide a comprehensive review on nanozyme-based aptasensors in food safety, which are arranged according to the different interaction modes of ssDNA and nanozymes: aptasensors based on nanozyme activity either inhibited or enhanced by ssDNA, nanozymes as signal tags, and other methods. Before introducing the nanozyme-based aptasensors, the regulation by ssDNA of nanozyme activity via diverse factors is discussed systematically for precisely tailoring nanozyme activity in biosensors. Furthermore, current challenges are emphasized, and future perspectives are discussed.

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Section: Nanozyme-based Aptasensorsmentioning

confidence: 70%

Regulation Mechanism of ssDNA Aptamer in Nanozymes and Application of Nanozyme-Based Aptasensors in Food Safety

Wang

Zhou

et al. 2022

Foods

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“…HGNs inherit the strong peroxidase activity of hemin and are less prone to environmental variations [ 23 , 24 ]. In previous studies, graphene-based biosensors have been demonstrated to have many merits, including the composition of multiple functions, choices of detection mechanism, signal generation, and amplification, as well as easy-engineering [ 25 , 26 , 27 , 28 ]. Particularly, biosensors using aptamers selected through systematic evolution of ligands by exponential enrichment (SELEX) have been developed specifically against targets with sizes varying from metal ions to cells [ 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

A Hemin–Graphene Nanocomposite-Based Aptasensor for Ultrasensitive Colorimetric Quantification of Leukaemia Cells Using Magnetic Enrichment

Zhang

Lai

et al. 2022

Biosensors

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Diagnostic blood cell counting is of limited use in monitoring a minimal number of leukaemia cells, warranting further research to develop more sensitive and reliable techniques to identify leukaemia cells in circulation. In this work, a hemin–graphene nanocomposite-based aptasensor was developed for ultrasensitive colorimetric detection of leukaemia cells (CEM) using magnetic enrichment. Hemin-conjugated graphene oxide nanocomposites (HGNs) were prepared by hydrazine reduction using graphene oxide nanosheets and hemins. Hence, the prepared HGNs become able to absorb single-stranded DNA and acquire peroxidase-like activity. The aptamer sgc8c, which recognizes a specific target on leukaemia cells, was absorbed onto HGNs to capture the target CEM cancer cells. The captured target cells that associated with the HGNs were then concentrated and separated by magnetic beads (MBs) coated with sgc8c aptamers, forming a HGN–cell–MB sandwich structure. These sandwich structures can be quantified via an oxidation reaction catalysed by HGNs. By utilizing dual signal amplification effects generated by magnetic enrichment and the improved peroxidase activity of HGNs, the biosensor allowed for highly sensitive detection of 10 to 105 CEM cells with an ultra-low limit of detection (LOD) of 10 cells under optimal conditions. It is expected that the proposed aptasensor can be further employed in monitoring the minimal residual disease during the treatment of leukaemia.

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Current progress in the development of biosensors based on nanomaterials for the detection of inorganic arsenic

Zheng,

Li,

Liu

et al. 2024

Int. J. Environ. Sci. Technol.

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A visual detection of bisphenol A based on peroxidase-like activity of hemin–graphene composites and aptamer

Abstract: Herein, a fast and visible colorimetric method for bisphenol A (BPA) detection was developed using hemin-functionalized reduced graphene oxide (H–rGO) composites and aptamer.

Cited by 19 publications

References 41 publications

Regulation Mechanism of ssDNA Aptamer in Nanozymes and Application of Nanozyme-Based Aptasensors in Food Safety

Regulation Mechanism of ssDNA Aptamer in Nanozymes and Application of Nanozyme-Based Aptasensors in Food Safety

A Hemin–Graphene Nanocomposite-Based Aptasensor for Ultrasensitive Colorimetric Quantification of Leukaemia Cells Using Magnetic Enrichment

Current progress in the development of biosensors based on nanomaterials for the detection of inorganic arsenic

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