A warm welcome for alternative <scp>CO</scp><sub>2</sub> fixation pathways in microbial biotechnology

Claassens, Nico J.

doi:10.1111/1751-7915.12456

Cited by 39 publications

(26 citation statements)

References 19 publications

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“…Using lignocelluloses as alternative feedstock in biorefinery has an apparent advantage by not competing with human consumption needs, but still faces many technical challenges, [2]. The utilization of one-carbon (C1) compounds, such as CO 2 [3][4][5], methane [6], methanol [7] and formate [8], has attracted much attention because they are naturally abundant, or available as industrial by-products and are thus cheap for the production of high-value chemicals. Beyond that, the biological assimilation of C1 compounds for the production of value-added chemicals can help reduce the emission of greenhouse gases such as CO 2 and methane which are largely responsible for global warming and climate change [4].…”

Section: Introductionmentioning

confidence: 99%

Formaldehyde formation in the glycine cleavage system and its use for an aldolase-based biosynthesis of 1,3-propanediol

Meng

Ren

et al. 2020

J Biol Eng

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Glycine cleavage system (GCS) occupies a key position in one-carbon (C1) metabolic pathway and receives great attention for the use of C1 carbons like formate and CO 2 via synthetic biology. In this work, we demonstrate that formaldehyde exists as a substantial byproduct of the GCS reaction cycle. Three causes are identified for its formation. First, the principal one is the decomposition of N 5 ,N 10-methylene-tetrahydrofolate (5,10-CH 2-THF) to form formaldehyde and THF. Increasing the rate of glycine cleavage promotes the formation of 5,10-CH 2-THF, thereby increasing the formaldehyde release rate. Next, formaldehyde can be produced in the GCS even in the absence of THF. The reason is that T-protein of the GCS can degrade methylamine-loaded H-protein (H int) to formaldehyde and ammonia, accompanied with the formation of dihydrolipoyl H-protein (H red), but the reaction rate is less than 0.16% of that in the presence of THF. Increasing T-protein concentration can speed up the release rate of formaldehyde by H int. Finally, a certain amount of formaldehyde can be formed in the GCS due to oxidative degradation of THF. Based on a formaldehyde-dependent aldolase, we elaborated a glycine-based one carbon metabolic pathway for the biosynthesis of 1,3-propanediol (1,3-PDO) in vitro. This work provides quantitative data and mechanistic understanding of formaldehyde formation in the GCS and a new biosynthetic pathway of 1,3-PDO.

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Section: Introductionmentioning

confidence: 99%

Formaldehyde formation in the glycine cleavage system and its use for an aldolase-based biosynthesis of 1,3-propanediol

Meng

Ren

et al. 2020

J Biol Eng

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“…However, they still grow as mixotrophs, requiring an organic carbon source for the production of the starting substrates of linear CO 2 fixation pathways and/or for the regeneration of ATP and/ or electron donors. Converting these engineered heterotrophs into true autotrophs would require the functional transplantation of complete CO 2 fixation cycles and the transplantation of, and integration with, energy-harvesting systems (Claassens et al, 2016;Claassens, 2017).…”

Section: Rational Consortia Between Photoautotrophs and Heterotrophsmentioning

confidence: 99%

About how to capture and exploit the CO₂ surplus that nature, per se, is not capable of fixing

Godoy

Mongili

Fino

et al. 2017

Microbial Biotechnology

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SummaryHuman activity has been altering many ecological cycles for decades, disturbing the natural mechanisms which are responsible for re‐establishing the normal environmental balances. Probably, the most disrupted of these cycles is the cycle of carbon. In this context, many technologies have been developed for an efficient CO 2 removal from the atmosphere. Once captured, it could be stored in large geological formations and other reservoirs like oceans. This strategy could present some environmental and economic problems. Alternately, CO 2 can be transformed into carbonates or different added‐value products, such as biofuels and bioplastics, recycling CO 2 from fossil fuel. Currently different methods are being studied in this field. We classified them into biological, inorganic and hybrid systems for CO 2 transformation. To be environmentally compatible, they should be powered by renewable energy sources. Although hybrid systems are still incipient technologies, they have made great advances in the recent years. In this scenario, biotechnology is the spearhead of ambitious strategies to capture CO 2 and reduce global warming.

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“…A wonderful alternative would be to exploit autotrophic microorganisms such as acetogenic anaerobic bacteria or microalgae (Schiel-Bengelsdorf and Durre, 2012;Scaife et al, 2015) as they have the capability to capture atmospheric CO 2 . However, these biological systems are at the moment industrially inefficient, due to their slow growth, poor productivity and low energy conversion yield (Claassens, 2017). The purpose of this mini-review is to expose and discuss original strategies that have emerged recently and that have employed synthetic biology tools to rewire the carbon metabolism of heterotrophic microorganisms to achieve maximal carbon conservation during their metabolism.…”

Section: Introductionmentioning

confidence: 99%

Synthetic Biology Applied to Carbon Conservative and Carbon Dioxide Recycling Pathways

François

Lachaux²,

Morin

2020

Front. Bioeng. Biotechnol.

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The global warming conjugated with our reliance to petrol derived processes and products have raised strong concern about the future of our planet, asking urgently to find sustainable substitute solutions to decrease this reliance and annihilate this climate change mainly due to excess of CO 2 emission. In this regard, the exploitation of microorganisms as microbial cell factories able to convert non-edible but renewable carbon sources into biofuels and commodity chemicals appears as an attractive solution. However, there is still a long way to go to make this solution economically viable and to introduce the use of microorganisms as one of the motor of the forthcoming bio-based economy. In this review, we address a scientific issue that must be challenged in order to improve the value of microbial organisms as cell factories. This issue is related to the capability of microbial systems to optimize carbon conservation during their metabolic processes. This initiative, which can be addressed nowadays using the advances in Synthetic Biology, should lead to an increase in products yield per carbon assimilated which is a key performance indice in biotechnological processes, as well as to indirectly contribute to a reduction of CO 2 emission.

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A warm welcome for alternative CO₂ fixation pathways in microbial biotechnology

Cited by 39 publications

References 19 publications

Formaldehyde formation in the glycine cleavage system and its use for an aldolase-based biosynthesis of 1,3-propanediol

Formaldehyde formation in the glycine cleavage system and its use for an aldolase-based biosynthesis of 1,3-propanediol

About how to capture and exploit the CO₂ surplus that nature, per se, is not capable of fixing

Synthetic Biology Applied to Carbon Conservative and Carbon Dioxide Recycling Pathways

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A warm welcome for alternative CO2 fixation pathways in microbial biotechnology

Cited by 39 publications

References 19 publications

Formaldehyde formation in the glycine cleavage system and its use for an aldolase-based biosynthesis of 1,3-propanediol

Formaldehyde formation in the glycine cleavage system and its use for an aldolase-based biosynthesis of 1,3-propanediol

About how to capture and exploit the CO2 surplus that nature, per se, is not capable of fixing

Synthetic Biology Applied to Carbon Conservative and Carbon Dioxide Recycling Pathways

Contact Info

Product

Resources

About

A warm welcome for alternative CO₂ fixation pathways in microbial biotechnology

About how to capture and exploit the CO₂ surplus that nature, per se, is not capable of fixing