A water extract of Malva verticillata seeds suppresses osteoclastogenesis and bone resorption stimulated by RANK ligand

Abstract

Background: Malva verticillata seeds are used as a therapeutic medicine to treat kidney dysfunction in traditional Chinese medicine (TCM). TCM has suggested that herbal medicine tonifying kidney function may have beneficial effect on bone metabolism. Methods: Osteoclastogenesis was examined in bone marrow macrophages by measuring tartrate-resistant acid phosphatase (TRAP) activity and counting the number of TRAP-stained multinuclear cells. The activation of receptor activator of nuclear factor-kB (RANK) ligand signaling, and the expression of c-Fos and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) were investigated by western blot analysis. Transcription factor and bone resorption marker mRNA levels were evaluated using real-time quantitative polymerase chain reaction. The bone resorption activity of mature osteoclast was examined in osteoclasts cultured on a hydroxyapatite-coated culture plate.

Devil's claw (Harpagophytum procumbens) and chronic inflammatory diseases: A concise overview on preclinical and clinical data

“…Medicinal plants displaying antioxidant and protective effects could represent a new research field in the osteoporosis management (Xiao, Zhang, Tong, & Shi, ). Several studies suggested that antioxidant‐rich plants may regulate osteoblastic and osteoclastic functions, with protective effects on bone matrix (Kim et al, ; Menghini et al, ; Shim, Lee, Yim, Ha, & Ma, ). Despite revealing minor efficacy compared with synthetic drugs, adverse effects are rare (Tabatabaei‐Malazy, Larijani, & Abdollahi, ).…”

Effect of the Extract and Constituents From Hancornia speciosa Fruits in Osteoclasts

“…4a). Other plant extracts have also been reported to elicit a decrease in the resorptive activity of osteoclasts, without interfering with their number [17,18]. Herein, we demonstrate for the first time that a fraction derived from EEF of H. speciosa fruits was able to decrease osteoclast activity.…”

“…Some compounds have indeed been described to inhibit osteoclast resorption activity. In this regard, a water extract from Malva verticillata L. (Malvaceae) seeds inhibited osteoclastogenesis stimulated by RANKL in bone marrow macrophages [17]. Moreover, a water extract from Acer tegmentosum Maxim (Sapindaceae) stems demonstrated a protective effect on bone destruction by inhibiting osteoclast differentiation and function [18].…”