A Water‐Soluble Copper(II) Complex for the Selective Fluorescence Detection of Nitric Oxide/Nitroxyl and Imaging in Living Cells

Abstract

An ovel water-soluble copper(II) naphthalimidec omplex fluorescent probe was devised and displayed significant fluorescence turn-on response for nitric oxide and nitroxyl over other biologically relevant reactive oxygen species under physiological conditions. The dual-analyte recognition of this simple and sensitivep robe was successfully appliedf or the imaging of exogenous and endogenous NO and HNO in living cells.[a] Dr.

Organic Nanoprobes for Fluorescence and 19F Magnetic Resonance Dual‐Modality Imaging

“…[3,4] Among the various combination of imaging modalities, a combination of fluorescence imaging (FI) and magnetic resonance imaging (MRI) [5][6][7][8][9][10][11] has the characteristic of complementary advantages. [12,13] Compared with other bioimaging techniques, FI displays more advantages of high sensitivity, easy operation, and cost-effectiveness, [14][15][16][17][18][19] which can also trace real-time cellular processes over a long period of time but lacks penetration depth for the internal organs, especially in view of in vivo applications. [20][21][22] With respect to MRI technique, [23][24][25] it is noninvasive and nondestructive, and can offer high spatial resolution, whereas it shows low sensitivity.…”

“…[26][27][28][29] To date, some exogenous contrast agents such as molecular probes with a number of equivalent fluorine atoms, [27,[30][31][32][33][34][35] polymeric tracers containing high numbers of fluorine atoms, [28,[36][37][38][39][40][41][42] and fluorinated metallic nanoparticles [43][44][45][46] have been developed and employed for 19 F MRI. Despite great successes, it is highly desirable and still challenging to develop suitable 19 F MRI contrast agents with satisfied performance as well as multi-modalities.…”

“…Herein, we report a new dual-modality imaging polymer nanoplatform for both fluorescence imaging (FI) and 19 F magnetic resonance imaging ( 19 F MRI). A fluorinated hydrophilic polymer (Pn, n＝1-7) was prepared through a simple atom-transfer radical polymerization (ATRP) strategy by employing 2,2,2-trifluoroethyl acrylate (TFEA) and poly(ethylene glycol) methyl ether acrylate (PEGA) as monomers, where the half width of the 19 F resonance peak could be adjusted within a certain range by simply changing the feed ratios of monomers.…”

“…[47][48][49] Thereafter, the obtained amphiphilic polymer (TPE-azo-Pn) self-assembled into a polymer TPE-Pn nanoprobe (TPE-PnN) via ultrasonication in aqueous solution, shown in Scheme 1. These nanoprobes were then utilized for both fluorescence imaging and 19 F magnetic resonance imaging.…”

“…[47] As indicated in Figure 1, TPE-N 3 displayed weak fluorescence in pure dimethylsulfoxide (DMSO) as well as in DMSO-H 2 O mixture with water fraction less than 30%, while the emission intensity significantly increased once with water fraction higher than 40%, which was originated from the poor solvent induced aggregation of TPE-N 3 . To obtain strong and sharp 19 F NMR peaks, and thus good 19 F MRI performance, it is important to increase the fluorine content per nanoprobe while ensure sufficient mobility of the fluorine moieties. [27,44] Therefore, the design principle for the polymer 19 F MRI nanoprobe is to prepare an amphiphilic fluorinated chain with a hydrophobic core and fluorine containing hydrophilic arms.…”