A Water Soluble CoQ10 Formulation Improves Intracellular Distribution and Promotes Mitochondrial Respiration in Cultured Cells

Bergamini, Christian; Moruzzi, Noah; Sblendido, Antonella; Lenaz, Giorgio; Fato, Romana

doi:10.1371/journal.pone.0033712

Cited by 75 publications

(64 citation statements)

References 46 publications

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“…This means that CoQ is physiologically not saturating and that increasing CoQ concentration in the membrane is bound to enhance respiratory activity: this is indeed what was found in the study with the watersoluble formulation quoted above [Bergamini et al, 2012]. Addition of Qter to cultured cells increased CoQ concentration in their mitochondria and significantly enhanced respiratory rates.…”

Section: What We Expect From Coq Deficiency and Supplementationsupporting

confidence: 66%

“…The major problem of CoQ 10 administration is its low bioavailability due to its extreme hydrophobicity [Beg et al, 2010]. A water-soluble formulation (Qter) has recently been shown to be easily incorporated into cultured cells and their mitochondria, enhancing respiration and antioxidant properties [Bergamini et al, 2012]. The same formulation was found to improve grip strength and to inhibit apoptosis in aged rats [Xu et al, 2010].…”

Section: What We Expect From Coq Deficiency and Supplementationmentioning

confidence: 99%

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Coenzyme Q and the Respiratory Chain: Coenzyme Q Pool and Mitochondrial Supercomplexes

Enrı́quez

Lenaz

2014

Mol Syndromol

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Two alternative models of organization of the mitochondrial electron transport chain (mETC) have been alternatively favored or questioned by the accumulation evidences of different sources, the solid model or the random collision model. Both agree in the number of respiratory complexes (I-IV) that participate in the mETC, but while the random collision model proposes that Complexes I-IV do not interact physically and that electrons are transferred between them by coenzyme Q and cytochrome c, the solid model proposes that all complexes super-assemble in the so-called respirasome. Recently, the plasticity model has been developed to incorporate the solid and the random collision model as extreme situations of a dynamic organization, allowing super-assembly free movement of the respiratory complexes. In this review, we evaluate the supporting evidences of each model and the implications of the super-assembly in the physiological role of coenzyme Q.

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Section: What We Expect From Coq Deficiency and Supplementationsupporting

confidence: 66%

Section: What We Expect From Coq Deficiency and Supplementationmentioning

confidence: 99%

Coenzyme Q and the Respiratory Chain: Coenzyme Q Pool and Mitochondrial Supercomplexes

Enrı́quez

Lenaz

2014

Mol Syndromol

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“…6 Reactive oxygen species were measured using the mitochondrial superoxide indicator MitoSOX Red, as previously described. 20 Briefly, CD34 + Mito Low and CD34 + Mito High cells were treated with 3 mM MitoSOX for 20 min and were then washed twice in Hanks balanced salt solution and analyzed by flow cytometry (Online Supplementary Figure S1A). …”

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confidence: 99%

Cord blood-derived CD34+ hematopoietic cells with low mitochondrial mass are enriched in hematopoietic repopulating stem cell function

et al. 2013

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ABSTRACT© F e r r a t a S t o r t i F o u n d a t i o n . logical role in a range of hematologic diseases, such as inherited dyserythropoiesis, sideroblastic anemias and low-grade myelodysplastic symdromes. 8,12 In addition, transcriptome, epigenetic and proteomic studies in stem cell systems have indicated that specific metabolic/mitochondrial properties are essential for regulating the balance between self-renewal and differentiation. 13,14 Although recent work has begun to shed light on the mitochondrial response during murine stem cell differentiation, 7,10,15,16 how and to what extent the mitochondrial mass/function contributes to human hematopoietic stem and progenitor function remains poorly understood. Here, we found that mitochondrial mass correlates strongly with mitochondrial membrane potential (ΔΨm Design and Methods Cord blood collection and CD34 + cell isolation and cultureFresh umbilical cord blood units from healthy neonates were obtained from local hospitals following approval from our local Ethics and Biohazard Board Committee. The cord blood samples were pooled to reduce variability among individual units. Mononuclear cells were isolated using Ficoll-Hypaque and after lysing the red cells (Cytognos, Salamanca, Spain), CD34 + cells were purified by magnetic bead separation using the human CD34 MicroBead kit and the AutoMACS Pro separator (Miltenyi Biotec) as instructed by the manufacturer. [17][18][19] The purity of the CD34 + fraction was assessed by flow cytometry using an anti-CD34-PE antibody (Miltenyi Biotec), and only CD34 + fractions showing purity higher than 90% were used. [17][18][19] The CD34 -fraction was irradiated (15 Gy) and used as accessory cells for co-transplantation with CD34 + cells. MitoTracker staining and cell sortingCD34 + cells were stained with MitoTracker Red (CMXRos) and MitoTracker Green FM dye (Molecular Probes) for 10 min and 20 min, respectively, according to the manufacturer's guidelines and analyzed by wide confocal cytometry. 6 For functional assays, CD34 + cells were FACS-sorted (FACSAria-II, BD Biosciences) based on MitoTracker Green levels into CD34 + Mito High and CD34 + Mito Low (n=10). Measurements of ATP and reactive oxygen speciesATP levels were measured using a Cell-Titer-Glo ® Luminescent Cell Viability Assay (Promega) according to the manufacturer's guidelines. Briefly, equal numbers of cells (5x10 4 /100 mL) were seeded in a 96-well plate and 100 mL of the reaction reagent were added to each well. After 10 min of shaking, the luminescence signal was detected using the GloMax ® -Multi Detection System (Promega) and compared against the ATP Standard Curve using ATP disodium salt (Promega). 6 Reactive oxygen species were measured using the mitochondrial superoxide indicator MitoSOX Red, as previously described. 20 Briefly, CD34 + Mito Low and CD34 + Mito High cells were treated with 3 mM MitoSOX for 20 min and were then washed twice in Hanks balanced salt solution and analyzed by flow cytometry (Online Supplementary Figure S1A). Gene expression by q...

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“…The authors had evaluated the bioenergetic effect of ubiquinone treatment, demonstrating that intracellular CoQ10 content after Qter supplementation positively correlates with an improved mitochondrial functionality (increased oxygen consumption rate, transmembrane potential, ATP synthesis) and resistance to oxidative stress. In conclusion, the improved cellular energy metabolism related to increase CoQ10 content represents a strong rationale for the clinical use of CoQ10 and highlights the biological effects of Qter, that make it the eligible CoQ10 formation for the ubiquinone supplementation [26] . Kim EA.…”

Section: The Bioavailability Of Coq10 With Aloe Veramentioning

confidence: 92%

Possible Prophylaxes of Aloe Vera Juice with CoQ10 to Enhance Muscle Performance

Yagi

Ataka

2016

Journal of Gastroenterology and Hepatology Research

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Coenzyme Q (CoQ10) is a major antioxidant principle found in human body which plays a vital role in maintaining several biochemical pathways of body. Deficiency of CoQ10 in body leads to several potential disorders in oxido-reductive reactions of electron transport chain. Present review offers preliminary insight into using dietary supplements, aloe vera juice with CoQ10, to support and optimize quality of life in the people suffering from muscle performance. A questionnaire on aloe vera juice with CoQ10 supplement was given to adult subjects. The questionnaire included 10 points regarding their mental, circulatory, and muscular functions, and the scoring was recorded from top six conditions in Table 1 and 2. Table 2 summarizes the noteworthy information of aloe vera juice with CoQ10 supplement. The high molecular weight and lipophilicity of CoQ10, making its poor water solubility and leading to low systemic availability, may be reduced by supplementation of aloe vera juice with CoQ10. Possible prophylaxes of aloe vera juice with CoQ10 supplement to enhance muscle performance are fully expected.

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A Water Soluble CoQ10 Formulation Improves Intracellular Distribution and Promotes Mitochondrial Respiration in Cultured Cells

Cited by 75 publications

References 46 publications

Coenzyme Q and the Respiratory Chain: Coenzyme Q Pool and Mitochondrial Supercomplexes

Coenzyme Q and the Respiratory Chain: Coenzyme Q Pool and Mitochondrial Supercomplexes

Cord blood-derived CD34+ hematopoietic cells with low mitochondrial mass are enriched in hematopoietic repopulating stem cell function

Possible Prophylaxes of Aloe Vera Juice with CoQ10 to Enhance Muscle Performance

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