A Water-soluble Fluorescent Probe for Rapid Detection of Sulfur Dioxide Derivatives

Abstract

The content of sulfur dioxide derivatives in cells is closely related to life and health. Therefore, it is essential to detect the content of sulfur dioxide derivatives in cells under physiological conditions to ensure life and health. In this paper, a novel sulfur dioxide derivative fluorescent water-soluble probe ((E)-1,1,3-trimethyl-2-(2-(naphthalen-1-yl)vinyl)-1H-3λ 4-benzo[e]indole, TNB) was synthesized by using naphthalene formaldehyde (1) and 1,1,2,3-tetramethyl-1H-3λ 4benzo[e] indole (2) as raw materials. Based on the intramolecular charge transfer (ICT) mechanism of the naphthalene ring to benzoindole, TNB exhibits very weak fluorescence emission in PBS buffer (pH 7.4). The olefin unit of TNB can be combined with HSO3-/SO3 2with high selectivity to make the π-π conjugate interrupt. ICT is blocked, TNB produces a strong fluorescence emission, and the color visible to the naked eye changes from yellow to colorless. The effect of TNB on HSO3-/SO3 2can be completed in 40 s, the fluorescence intensity is increased by 91 times, and the detection limit is as low as 0.089 μM. It is expected to be able to rapidly detect low levels of sulfur dioxide derivatives in cells, which has important application prospects in maintaining life and health.

A highly sensitive fluorescent probe for ozone based on coumarin-benzothiazole derivative

“…3b). According to the detection limit formula LOD = 3σ/k (Where σ is the standard deviation of the blank sample measurement, k is the slope between fluorescence intensity and O 3 concentration) (Zhao et al 2020), the minimum detection concentration is calculated to be 43 nM, which indicates that the probe can be used to detect trace amounts of O 3 in a physiological environment.…”

Simple Fluorescence Assay for Triethylamine Based on the Palladium Catalytic Dimerization of Benzofuran-2-boronic Acid

“…Although several gas chromatographic methods have been developed, these methods require relatively complicated instruments. [13][14][15] Further, although the fluorescence method has advantages in terms of simplicity, sensitivity and rapidness, 16 TEA does not exhibit intrinsic fluorescence, and it should be difficult to apply fluorescence derivatization because TEA does not have a derivatizable moiety. 17 From these aspects, we attempted to develop a simple and rapid fluorescence microplate assay for TEA based on promoting the dimerization of benzofuran-2-boronic acid.…”

Stimuli-responsive Behaviors for Room-temperature Fluorescent Liquid Materials based on N-Heteroacenes and their Mixtures in Response to HCl Vapor and their Facile Synthesis

“…As hydrophilic liquid materials can be dissolved in or miscible to water media, they are expected to function as sensing materials to highly toxic metals. 17,[39][40]45 Analytical Sciences Figure 4a-c, blue lines indicate that proBz was in air for 1h, and proPy and proPhen were in air for 1 day.…”

“…Furthermore, the chemical modification such as a water solubility into these materials can provide the opportunity to use for colorimetric fluorescent probes in various fields. 17,[39][40][41][42][43][44][45] Herein, we report on facile synthesis for a series of SFLs with a low environmental load as well as the characterization of mixtures with SFLs.…”