A weak cation‐exchange monolith as stationary phase for the separation of peptide diastereomers by CEC

Ludewig, Ronny; Nietzsche, Sándor; Scriba, Gerhard K. E.

doi:10.1002/jssc.201000616

Cited by 12 publications

(11 citation statements)

References 29 publications

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“…Analyte separation was done at mobile phases consisting of acidic phosphate buffer and acetonitrile. A comparison between the weak‐cation exchange monolith with the reversed‐phase monolith and a strong cation‐exchange monolith showed different order of elution for some of the peptide diastereomers, so it was concluded that interactions with the stationary phase contribute to the CEC separations are different .…”

Section: Monolithic Stationary Phasesmentioning

confidence: 99%

Capillary electrochromatography of proteins and peptides (2006–2015)

Mikšı́k

2016

J of Separation Science

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This review summarizes the development and the applications of capillary electrochromatographic techniques pertinent to the analysis of proteins and peptides, which took place over the last 10 years (2006-mid-2016). This "hybrid" technique is useful for the analysis of a broad spectrum of proteins and peptides and is an approach that is complementary to the liquid chromatographic and the capillary electrophoretic analysis. All modes of capillary electrochromatography are described here, which includes particle-packed columns, monolithic stationary phases, and open-tubular capillary electrochromatography.

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Section: Monolithic Stationary Phasesmentioning

confidence: 99%

Capillary electrochromatography of proteins and peptides (2006–2015)

Mikšı́k

2016

J of Separation Science

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“…Generally, three kinds of stationary phases have been used in CEC: packed particle beds, monolithic columns and thin layers of stationary phases immobilized to the inner wall of the capillary (so called open tubular CEC, OT-CEC). For peptide diastereomer separations only OT-CEC (155) and copolymer monoliths (171,172) have been utilized (Table 10).…”

Section: Capillary Electrochromatographymentioning

confidence: 99%

Separation of Peptide Diastereomers

Scriba

2014

Encyclopedia of Analytical Chemistry

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Diastereomeric peptides, isomers in which one or more of the chiral centers have been converted to the opposite configuration (R or S, D or L ), often possess different biological activities and/or conformational properties. Therefore, the separation and determination of diastereomeric peptides is important in life sciences and particularly significant to the pharmaceutical industry for quality control of peptide synthesis and stability as well as for regulatory requirements. Diastereomers differ in their physicochemical properties that can be utilized for their determination by numerous techniques. In contrast to many other methods such as optical rotation, circular dichroism, differential scanning calorimetry, or infrared (IR) spectroscopy, separation techniques such as chromatography or capillary electrophoresis (CE) allow the sensitive and simultaneous identification and quantification of peptide diastereomers. This chapter covers analytical‐scale separation of peptide diastereomers by chromatographic methods, including paper chromatography (PC), thin‐layer chromatography (TLC), gas chromatography (GC), high‐performance liquid chromatography (HPLC), and CE. HPLC is currently the predominantly used method for peptide diastereomer separations. However, owing to the high resolving power, particularly for polar compounds, CE has been applied to the separation of diastereomeric peptides in recent years. The chapter focuses on methods employing achiral stationary phases or buffers without chiral additives for the separations, although some examples of such separations, i.e. the use of chiral columns or chiral mobile phase additives, are mentioned. Moreover, only diasteromeric peptides containing natural amino acids will be discussed.

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“…Monoliths can be inorganic, often based on silica, [4][5][6] or organic, based on methacrylates, styrenes or acrylamides. [7][8][9][10][11] Methacrylatebased monoliths can be prepared having different surface hydrophobicities, depending on the monomer used. The most used methacrylate monomers for synthesis of monolithic stationary phases for use in CEC use C4 (butyl methacrylate), [12,13] C12 (lauryl methacrylate) [7] and C18 (octadecyl methacrylate) [8] substituents.…”

Section: Introductionmentioning

confidence: 99%

“…The most used methacrylate monomers for synthesis of monolithic stationary phases for use in CEC use C4 (butyl methacrylate), [12,13] C12 (lauryl methacrylate) [7] and C18 (octadecyl methacrylate) [8] substituents. Organic monoliths are chemically stable over a wide range of pH (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12), a stability that is not possible with the types of silica presently on the market, which dissolves in highly basic media. Careful control of the polymerization process (through variation in the type and proportion of porogenic solvents or variation in the proportion of these with respect to monomers) enables optimization of the porous properties and, consequently, of the efficiency and selectivity of the separation.…”

Section: Introductionmentioning

confidence: 99%

Repeatability of Octadecyl Methacrylate-Based Monolithic Columns for Capillary Electrochromatography

Aguiar

Bottoli

2015

Instrumentation Science & Technology

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& Octadecyl methacrylate-based monolithic capillary columns were prepared using octadecyl methacrylate as the monomer, ethylene dimethacrylate as the cross-linking agent, 2-acryloylamido-2-methylpropanesulfonic acid as the ionizable monomer, responsible for a negatively charged stationary phase surface and able to support a cathodic electrosmotic flow, and amyl alcohol and 1,4-butanediol as porogenic solvents. The repeatability of the morphological aspect of the monolithic material was evaluated by scanning electron microscopy (SEM). The uniformity along the same monolithic bed was also investigated by SEM. Replicate determinations of surface area and pore volume were also performed and compared. The repeatability of the obtained electrochromatographic efficiencies for separations of alkylbenzenes using the stationary phases prepared was also evaluated. A column that had a plate height of 31 lm was tested for separation of polycyclic aromatic hydrocarbons. Even though minimal possible variation of experimental parameters was maintained, it was not possible to reproduce the stationary phases of the monolithic columns, since the relative standard deviation for the efficiencies obtained on columns prepared from the same polymerization mixture composition was 62%. The lack of repeatability for the efficiency is thought to be a result of limited homogeneity of the monolithic globular structures along the length of the majority of the prepared columns. The poor precision of preparation was justified by the copolymerization reaction that occurs in the presence of 2, 2 0 -azobisisobutyronitrile as the initiator agent, whose decomposition starts at the moment that this is added to the reaction mixture.

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A weak cation‐exchange monolith as stationary phase for the separation of peptide diastereomers by CEC

Cited by 12 publications

References 29 publications

Capillary electrochromatography of proteins and peptides (2006–2015)

Capillary electrochromatography of proteins and peptides (2006–2015)

Separation of Peptide Diastereomers

Repeatability of Octadecyl Methacrylate-Based Monolithic Columns for Capillary Electrochromatography

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