In order to further dynamically update and maintain teaching resources and establish a teaching platform with various interactive modes, which is suitable for students, teachers, management and other levels to operate comprehensively, this paper constructs a Web-based experiment information teaching system by using the ideas and technologies of B/S mode. The system integrates the latest technology of the current network, integrates all kinds of teaching needs, rich teaching auxiliary functions and teaching management functions, and has strong pertinence and practicability. Based on the improved three-tier architecture, the system has good expansibility and maintainability, and can be easily transplanted to other teaching fields. The database design based on configurable view ensures the efficiency and security of the system. At present, the system has been widely used in many universities in China.