A web‐based interactive developmental table for the ascidian <i>Ciona intestinalis</i>, including 3D real‐image embryo reconstructions: I. From fertilized egg to hatching larva

Hotta, Kohji; Mitsuhara, Kenta; Takahashi, Hiroshi; Inaba, Kazuo; Oka, Kotaro; Gojobori, Takashi; Ikeo, Kazuho

doi:10.1002/dvdy.21188

Cited by 234 publications

(260 citation statements)

References 21 publications

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“…Imaging was performed in a room maintained at 20°C. We found no significant deviation from the developmental table (Hotta et al, 2007) defined at 18°C until 8.5 hours post fertilization (hpf). pSPCiEpi1GAP43C and pSPCiEpi1CiCdc25CiEpi1GAP43C (linearized with XhoI) were microinjected into unfertilized eggs together with mVenus-hGem(1/110) mRNA.…”

Section: Time-lapse Imaging Using Wide-field Microscopymentioning

confidence: 66%

Coordination of mitosis and morphogenesis: role of a prolonged G2 phase during chordate neurulation

et al. 2011

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SUMMARYChordates undergo a characteristic morphogenetic process during neurulation to form a dorsal hollow neural tube. Neurulation begins with the formation of the neural plate and ends when the left epidermis and right epidermis overlying the neural tube fuse to close the neural fold. During these processes, mitosis and the various morphogenetic movements need to be coordinated. In this study, we investigated the epidermal cell cycle in Ciona intestinalis embryos in vivo using a fluorescent ubiquitination-based cell cycle indicator (Fucci). Epidermal cells of Ciona undergo 11 divisions as the embryos progress from fertilization to the tadpole larval stage. We detected a long G2 phase between the tenth and eleventh cell divisions, during which fusion of the left and right epidermis occurred. Characteristic cell shape change and actin filament regulation were observed during the G2 phase. CDC25 is probably a key regulator of the cell cycle progression of epidermal cells. Artificially shortening this G2 phase by overexpressing CDC25 caused precocious cell division before or during neural tube closure, thereby disrupting the characteristic morphogenetic movement. Delaying the precocious cell division by prolonging the S phase with aphidicolin ameliorated the effects of CDC25. These results suggest that the long interphase during the eleventh epidermal cell cycle is required for neurulation.

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Section: Time-lapse Imaging Using Wide-field Microscopymentioning

confidence: 66%

Coordination of mitosis and morphogenesis: role of a prolonged G2 phase during chordate neurulation

et al. 2011

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“…Embryos were fixed mid-TVC migration (10.5 h post fertilization, hpf; Hotta et al, 2007) and after hatching (24.5 hpf ). No positive TUNEL staining in any condition was visible at 10.5 hpf (supplementary material Fig.…”

Section: Tissue-specific Inhibition Of the Secretory Pathwaymentioning

confidence: 99%

Surrounding tissues canalize motile cardiopharyngeal progenitors towards collective polarity and directed migration

et al. 2015

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Collectively migrating cells maintain group polarity and interpret external cues to reach their destination. The cardiogenic progenitors (also known as trunk ventral cells, TVCs) of the ascidian Ciona intestinalis provide a simple chordate model with which to study collective migration. Bilateral pairs of associated TVCs undergo a stereotyped polarized migration away from the tail towards the ventral trunk, arguably constituting the simplest possible example of directed collective migration. To identify tissues contributing to TVC polarity and migration, we quantified the contact between TVCs and surrounding tissues, and blocked the secretory pathway in a tissuespecific manner. Even though TVCs normally migrate as an invariably determined leader-trailer polarized pair of adherent cells, they are capable of migrating individually, albeit a shorter distance and with altered morphology. The mesenchyme contacts newborn TVCs and contributes to robust specification of the trailer but appears to have only minor effects on directed migration. The notochord does not contact the TVCs but contributes to the onset of migration. The trunk endoderm first contacts the leader TVC, then 'encases' both migrating cells and provides the inputs maintaining leader-trailer polarity. Migrating TVCs adhere to the epidermis and need this contact for their cohesion. These phenomenological studies reveal that inherently motile cardiopharyngeal progenitors are channeled into stereotyped behaviors by interactions with surrounding tissues.

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“…Collection, rearing, fertilization, staging, dechorionation and electroporation were carried out as previously described (Corbo et al, 1997;Davidson et al, 2005;Hotta et al, 2007).…”

Section: Embryological Techniquesmentioning

confidence: 99%

Matrix adhesion polarizes heart progenitor induction in the invertebrate chordate Ciona intestinalis

et al. 2013

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SUMMARYCell-matrix adhesion strongly influences developmental signaling. Resulting impacts on cell migration and tissue morphogenesis are well characterized. However, the in vivo impact of adhesion on fate induction remains ambiguous. Here, we employ the invertebrate chordate Ciona intestinalis to delineate an essential in vivo role for matrix adhesion in heart progenitor induction. In Ciona precardiac founder cells, invasion of the underlying epidermis promotes localized induction of the heart progenitor lineage. We found that these epidermal invasions are associated with matrix adhesion along the pre-cardiac cell/epidermal boundary. Through targeted manipulations of RAP GTPase activity, we were able to manipulate pre-cardiac cell-matrix adhesion. Targeted disruption of precardiac cell-matrix adhesion blocked heart progenitor induction. Conversely, increased matrix adhesion generated expanded induction. We were also able to selectively restore cell-matrix adhesion and heart progenitor induction through targeted expression of CiIntegrin β2. These results indicate that matrix adhesion functions as a necessary and sufficient extrinsic cue for regional heart progenitor induction. Furthermore, time-lapse imaging suggests that cytokinesis acts as an intrinsic temporal regulator of heart progenitor adhesion and induction. Our findings highlight a potentially conserved role for matrix adhesion in early steps of vertebrate heart progenitor specification.

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A web‐based interactive developmental table for the ascidian Ciona intestinalis, including 3D real‐image embryo reconstructions: I. From fertilized egg to hatching larva

Cited by 234 publications

References 21 publications

Coordination of mitosis and morphogenesis: role of a prolonged G2 phase during chordate neurulation

Coordination of mitosis and morphogenesis: role of a prolonged G2 phase during chordate neurulation

Surrounding tissues canalize motile cardiopharyngeal progenitors towards collective polarity and directed migration

Matrix adhesion polarizes heart progenitor induction in the invertebrate chordate Ciona intestinalis

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