A WEE1 homologue from Arabidopsis thaliana

Sorrell, David A.; Marchbank, Angela; McMahon, Kathryn; Dickinson, Rebecca; Rogers, Hilary J.; Francis, Dennis

doi:10.1007/s00425-002-0815-4

Cited by 93 publications

(80 citation statements)

References 17 publications

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“…No significant difference in cell size was observed for the different constructs under noninducing conditions. In agreement with previously published results, in the absence of thiamine, expression of the full-length WEE1 gene clearly interfered with the yeast cell cycle, resulting in an elongated cell phenotype ( Figure 4B) (Sun et al, 1999;Sorrell et al, 2002). By contrast, expression of the truncated wee1 alleles did not arrest the yeast cell cycle, because no difference in cell size was observed between cells grown in the presence or absence of thiamine.…”

Section: Wee1 Activity Is Not Required For Cell Division or Endoredupsupporting

confidence: 92%

“…Multiple CDKs and cyclins are encoded by the genomes of Arabidopsis thaliana and Oryza sativa (rice) (Vandepoele et al, 2002;Wang et al, 2004;La et al, 2006). In addition, a WEE1-related kinase has been described for maize (Zea mays), tomato (Solanum lycopersicum), and Arabidopsis (Sun et al, 1999;Sorrell et al, 2002;Gonzalez et al, 2004). Although the plant WEE1 gene is unable to complement mutations in its yeast homolog, its overexpression inhibits cell division in fission yeast.…”

Section: Introductionmentioning

confidence: 99%

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ArabidopsisWEE1 Kinase Controls Cell Cycle Arrest in Response to Activation of the DNA Integrity Checkpoint

Schutter¹,

Joubès²,

et al. 2007

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Upon the incidence of DNA stress, the ataxia telangiectasia-mutated (ATM) and Rad3-related (ATR) signaling kinases activate a transient cell cycle arrest that allows cells to repair DNA before proceeding into mitosis. Although the ATM-ATR pathway is highly conserved over species, the mechanisms by which plant cells stop their cell cycle in response to the loss of genome integrity are unclear. We demonstrate that the cell cycle regulatory WEE1 kinase gene of Arabidopsis thaliana is transcriptionally activated upon the cessation of DNA replication or DNA damage in an ATR-or ATM-dependent manner, respectively. In accordance with a role for WEE1 in DNA stress signaling, WEE1-deficient plants showed no obvious cell division or endoreduplication phenotype when grown under nonstress conditions but were hypersensitive to agents that impair DNA replication. Induced WEE1 expression inhibited plant growth by arresting dividing cells in the G2-phase of the cell cycle. We conclude that the plant WEE1 gene is not rate-limiting for cycle progression under normal growth conditions but is a critical target of the ATR-ATM signaling cascades that inhibit the cell cycle upon activation of the DNA integrity checkpoints, coupling mitosis to DNA repair in cells that suffer DNA damage.

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Section: Wee1 Activity Is Not Required For Cell Division or Endoredupsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

ArabidopsisWEE1 Kinase Controls Cell Cycle Arrest in Response to Activation of the DNA Integrity Checkpoint

Schutter¹,

Joubès²,

et al. 2007

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show abstract

“…In addition, although no protein with similarities to Myt1/Mik1 + could be isolated in plants, a Wee1 homolog has been identified in different plant species (Sun et al, 1999;Sorrell et al, 2002;Gonzalez et al, 2004), and in in vitro experiments, WEE1 could phosphorylate CDK and block its activity (Sun et al, 1999;Shimotohno et al, 2006;Gonzalez et al, 2007). RNA interference-mediated downregulation of WEE1 expression in tomato resulted in reduced plant growth and a smaller fruit size (Gonzalez et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Control of Cell Proliferation, Organ Growth, and DNA Damage Response Operate Independently of Dephosphorylation of the Arabidopsis Cdk1 Homolog CDKA;1

et al. 2009

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Entry into mitosis is universally controlled by cyclin-dependent kinases (CDKs). A key regulatory event in metazoans and fission yeast is CDK activation by the removal of inhibitory phosphate groups in the ATP binding pocket catalyzed by Cdc25 phosphatases. In contrast with other multicellular organisms, we show here that in the flowering plant Arabidopsis thaliana, cell cycle control does not depend on sudden changes in the phosphorylation pattern of the PSTAIRE-containing Cdk1 homolog CDKA;1. Consistently, we found that neither mutants in a previously identified CDC25 candidate gene nor plants in which it is overexpressed display cell cycle defects. Inhibitory phosphorylation of CDKs is also the key event in metazoans to arrest cell cycle progression upon DNA damage. However, we show here that the DNA damage checkpoint in Arabidopsis can also operate independently of the phosphorylation of CDKA;1. These observations reveal a surprising degree of divergence in the circuitry of highly conserved core cell cycle regulators in multicellular organisms. Based on biomathematical simulations, we propose a plant-specific model of how progression through the cell cycle could be wired in Arabidopsis.

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“…Plants lack an orthologous CDC25 gene (Boudolf et al, 2006) but possess a homolog of the WEE1 protein kinase (Sun et al, 1999;Sorrell et al, 2002;Gonzalez et al, 2004). Arabidopsis thaliana WEE1 transcript levels are strongly induced upon treatment with replication-inhibitory drugs in an ATR-dependent manner.…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis thaliana RNase H2 Deficiency Counteracts the Needs for the WEE1 Checkpoint Kinase but Triggers Genome Instability

Kalhorzadeh

Cools

et al. 2014

The Plant Cell

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The WEE1 kinase is an essential cell cycle checkpoint regulator in Arabidopsis thaliana plants experiencing replication defects. Whereas under non-stress conditions WEE1-deficient plants develop normally, they fail to adapt to replication inhibitory conditions, resulting in the accumulation of DNA damage and loss of cell division competence. We identified mutant alleles of the genes encoding subunits of the ribonuclease H2 (RNase H2) complex, known for its role in removing ribonucleotides from DNA-RNA duplexes, as suppressor mutants of WEE1 knockout plants. RNase H2 deficiency triggered an increase in homologous recombination (HR), correlated with the accumulation of g-H2AX foci. However, as HR negatively impacts the growth of WEE1-deficient plants under replication stress, it cannot account for the rescue of the replication defects of the WEE1 knockout plants. Rather, the observed increase in ribonucleotide incorporation in DNA indicates that the substitution of deoxynucleotide with ribonucleotide abolishes the need for WEE1 under replication stress. Strikingly, increased ribonucleotide incorporation in DNA correlated with the occurrence of small base pair deletions, identifying the RNase H2 complex as an important suppressor of genome instability.

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A WEE1 homologue from Arabidopsis thaliana

Cited by 93 publications

References 17 publications

ArabidopsisWEE1 Kinase Controls Cell Cycle Arrest in Response to Activation of the DNA Integrity Checkpoint

ArabidopsisWEE1 Kinase Controls Cell Cycle Arrest in Response to Activation of the DNA Integrity Checkpoint

Control of Cell Proliferation, Organ Growth, and DNA Damage Response Operate Independently of Dephosphorylation of the Arabidopsis Cdk1 Homolog CDKA;1

Arabidopsis thaliana RNase H2 Deficiency Counteracts the Needs for the WEE1 Checkpoint Kinase but Triggers Genome Instability

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