Electron beam welding (EBW) has been attracted due to its high energy density, high depth-to-width ratio, low thermal strain, compared with arc and laser welding. In addition, it ensures high quality weld joint in structural metals in a wide range of thickness from 0.025 mm to 300 mm. Therefore, EBW is widely used in industries such as automotive, aviation, nuclear power plant, etc. Although a lot of studies on EBW for several decades have been presented, EB based repair welding with supplement of filler metal has less reported. In this work, EB repair welding for 5083 aluminum alloys was investigated because the aluminum alloys are becoming more important as a lightweight material in mobility industries. Material characterization and mechanical properties of the repair-welded specimens were evaluated, compared with characteristics of base metal and bead welding without repair. The optimized experimental conditions showed tensile strength of 294 MPa, which is a similar to base metal. Our study indicates that EB repair welding can be considered for the candidate of repair welding of aluminum alloys.