A whole-cell electrochemical biosensing system based on bacterial inward electron flow for fumarate quantification

Si, Rong-Wei; Zhai, Dan‐Dan; Liao, Zhi-Hong; Gao, Lu; Yong, Yang‐Chun

doi:10.1016/j.bios.2014.12.035

Cited by 53 publications

(33 citation statements)

References 36 publications

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“…Apart from formate dehydrogenase and carbon-monoxide dehydrogenase complex, the remaining genes were organized in a cluster, whose gene . 3 SEM (a, b) of C. scatologenes ATCC 25775 after microbial electrosynthesis experiments ended in low and high scale, respectively content and organization were identical to that found in C. ljungdahlii (Si et al 2015) and C. difficile (Yang et al 2017). In the Wood-Ljungdahl pathway, hydrogen provides the electrons and protons required for the synthesis of the products.…”

Section: Discussionmentioning

confidence: 64%

“…Among all those technologies, the MES is considered as a promising technology for production of value-added chemicals and fuels, from CO 2 (Patil et al 2015). MES is an application of a bioelectrochemical system (Yong et al 2014;Yang et al 2017;Si et al 2015), in which anaerobic microbes can accept electrons, from a cathode, as an energy source and CO 2 , as a carbon source, to form high value-added products, including methane (Cheng et al 2009), hydrogen peroxide (Rozendal et al 2009), formic acid (Kim et al 2014), acetic acid (Huang et al 2014), ethanol (Steinbusch et al 2010), acetone (Kim and Kim 1988), butanol (Kim and Kim 1988), butyric acid, 2,3-butanedio (Nevin et al 2010), and so on. Therefore, an appropriate biocatalyst is the key factor affecting the transformation efficiency and the product spectrum in the MES (Bajracharya et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Microbial electrosynthesis of organic chemicals from CO2 by Clostridium scatologenes ATCC 25775T

Liu

Song

Fei

et al. 2018

Bioresour. Bioprocess.

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Background: The conversion of CO 2 into high value-added products has a very important environmental and economic significance. Microbial electrosynthesis (MES) is a promising technology, which adopts a bioelectrochemical system to transform CO 2 into organic chemicals. Results: In this study, Clostridium scatologenes ATCC 25775 T , an anaerobic acetogenic bacterium, demonstrated its utility as a biocatalyst in a MES system, for the first time. With the cathodic potential of the MES system decreased from − 0.6 to − 1.2 V (vs. Ag/AgCl), the current density of the MES, and the production of organic chemicals, increased. Combining the genetic analysis and the results of the wet lab experiments, we believe C. scatologenes may accept electrons directly from the cathode to reduce CO 2 into organic compounds at a potential of − 0.6 V. The acetic and butyric acid reached a maximum value of 0.03 and 0.01 g/L, respectively, and the maximum value of total coulombic efficiency was about 84%, at the potential of − 0.6 V. With the decrease in cathodic potentials, both direct electron transfer and exogenous electron shuttle, H 2 might be adopted for the C. scatologenes MES system. At a potential of − 1.2 V, acetic acid, butyric acid and ethanol were detected in the cathodic chamber, with their maximum values increasing to 0.44, 0.085 and 0.015 g/L, respectively. However, due to the low H 2 utilization rate by the C. scatologenes planktonic cell, the total coulombic efficiency of the MES system dropped to 37.8%. Conclusion: Clostridium scatologenes is an acetogenic bacterium which may fix CO 2 through the Wood-Ljungdahl pathway. Under H 2 fermentation, C. scatologenes may reduce CO 2 to acetic acid, butyric acid and ethanol. It can also be used as the biocatalyst in MES systems.

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Section: Discussionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Microbial electrosynthesis of organic chemicals from CO2 by Clostridium scatologenes ATCC 25775T

Liu

Song

Fei

et al. 2018

Bioresour. Bioprocess.

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“…In addition, several single‐species sensing system have been developed for, for example, monitoring of acetate by G. sulfurreducens (Tront et al ., 2008; Nevin et al ., 2011), sensing of lactate, fumarate and arabinose by S. oneidensis (Kouzuma et al ., 2012; Golitsch et al ., 2013; Si et al ., 2015) and the detection of arsenic by Enterobacter cloacae and S. oneidensis (Webster et al ., 2014; Rasmussen and Minteer, 2015). More advanced applications include the cell‐internal processing of quorum sensing signals which has been achieved by the creation of AND logic gates in S. oneidensis and P. aeruginosa (Li et al ., 2011; Hu et al ., 2015).…”

Section: Resultsmentioning

confidence: 99%

Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili

Lienemann

TerAvest

Pitkänen

et al. 2018

Microbial Biotechnology

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SummaryBiosensors detect signals using biological sensing components such as redox enzymes and biological cells. Although cellular versatility can be beneficial for different applications, limited stability and efficiency in signal transduction at electrode surfaces represent a challenge. Recent studies have shown that the Mtr electron conduit from Shewanella oneidensis MR‐1 can be produced in Escherichia coli to generate an exoelectrogenic model system with well‐characterized genetic tools. However, means to specifically immobilize this organism at solid substrates as electroactive biofilms have not been tested previously. Here, we show that mannose‐binding Fim pili can be produced in exoelectrogenic E. coli and can be used to selectively attach cells to a mannose‐coated material. Importantly, cells expressing fim genes retained current production by the heterologous Mtr electron conduit. Our results demonstrate the versatility of the exoelectrogenic E. coli system and motivate future work that aims to produce patterned biofilms for bioelectronic devices that can respond to various biochemical signals.

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“…Nevertheless, a cost-effective and fast analytical method for the detection and quantification of fumarate is desired. Si et al (2015) produced an electrochemical whole-cell biosensing system for the quantification of fumarate in foods (apple juice) (Si, Zhai, Liao, Gao, & Yong, 2015). A sensitive inwards electric output (electron flow from electrode into bacteria) is sensitive to fumarate in Shewanella oneidensis M A N U S C R I P T…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Potential use of electronic noses, electronic tongues and biosensors as multisensor systems for spoilage examination in foods

Ghasemi‐Varnamkhasti

Apetrei

Lozano

et al. 2018

Trends in Food Science & Technology

144

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Development and use of reliable and precise detecting systems in the food supply chain must be taken into account to ensure the maximum level of food safety and quality for consumers. Spoilage is a challenging concern in food safety considerations as it is a threat to public health and is seriously considered in food hygiene issues accordingly. Although some procedures and detection methods are already available for the determination of spoilage in food products, these traditional methods have some limitations and drawbacks as they are time-consuming, labour intensive and relatively expensive. Therefore, there is an urgent need for the development of rapid, reliable, precise and non-expensive systems to be used in the food supply and production chain as monitoring devices to detect metabolic alterations in foodstuff. Attention to instrumental detection systems such as electronic noses, electronic tongues and biosensors coupled with chemometric approaches has greatly increased because they have been demonstrated as a promising alternative for the purpose of detecting and monitoring food spoilage. This paper mainly focuses on the recent developments and the application of such multisensor systems in the food industry. Furthermore, the most traditionally methods for food spoilage detection are introduced in this context as well. The challenges and future trends of the potential use of the systems are also discussed. Based on the published literature, encouraging reports demonstrate that such systems are indeed the most promising candidates for the detection and monitoring of spoilage microorganisms in different foodstuff.

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A whole-cell electrochemical biosensing system based on bacterial inward electron flow for fumarate quantification

Cited by 53 publications

References 36 publications

Microbial electrosynthesis of organic chemicals from CO2 by Clostridium scatologenes ATCC 25775T

Microbial electrosynthesis of organic chemicals from CO2 by Clostridium scatologenes ATCC 25775T

Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili

Potential use of electronic noses, electronic tongues and biosensors as multisensor systems for spoilage examination in foods

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