A Wild Arachis Endochitinase Enhances Sclerotinia Resistance in Transgenic Plants

da Silva Ferreira, Deziany; da Cunha Quintana Martins, Andressa; Berbert, Pedro Souza; dos Anjos, Renan Miguel; de Passos Saraiva, Mario Alfredo; Brasileiro, Ana Cristina Miranda; Miller, Robert Neil Gerard; Guimaraes, Patricia Messenberg

doi:10.1007/s12042-024-09359-z

Tropical Plant Biol.

2024

DOI: 10.1007/s12042-024-09359-z

|View full text |Cite

A Wild Arachis Endochitinase Enhances Sclerotinia Resistance in Transgenic Plants

Deziany da Silva Ferreira,

Andressa da Cunha Quintana Martins,

Pedro Souza Berbert

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 102 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Ectopic expression of a truncated NLR gene from wild Arachis enhances resistance to Fusarium oxysporum

de Araújo,

Brasileiro,

Martins

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

Fusarium oxysporum causes devastating vascular wilt diseases in numerous crop species, resulting in substantial yield losses. The Arabidopsis thaliana-F. oxysporum f.sp. conglutinans (FOC) model system enables the identification of meaningful genotype–phenotype correlations and was applied in this study to evaluate the effects of overexpressing an NLR gene (AsTIR19) from Arachis stenosperma against pathogen infection. AsTIR19 overexpression (OE) lines exhibited enhanced resistance to FOC without any discernible phenotype penalties. To elucidate the underlying resistance mechanisms mediated by AsTIR19 overexpression, we conducted whole transcriptome sequencing of an AsTIR19-OE line and non-transgenic wild-type (WT) plants inoculated and non-inoculated with FOC using Illumina HiSeq4000. Comparative analysis revealed 778 differentially expressed genes (DEGs) attributed to transgene overexpression, while fungal inoculation induced 434 DEGs in the OE line, with many falling into defense-related Gene Ontology (GO) categories. GO and KEGG enrichment analysis showed that DEGs were enriched in the phenylpropanoid and flavonoid pathways in the OE plants. This comprehensive transcriptomic analysis underscores how AsTIR19 overexpression reprograms transcriptional networks, modulating the expression of stress-responsive genes across diverse metabolic pathways. These findings provide valuable insights into the molecular mechanisms underlying the role of this NLR gene under stress conditions, highlighting its potential to enhance resistance to Fusarium oxysporum.

show abstract

Ectopic expression of a truncated NLR gene from wild Arachis enhances resistance to Fusarium oxysporum

de Araújo,

Brasileiro,

Martins

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

A Wild Arachis Endochitinase Enhances Sclerotinia Resistance in Transgenic Plants

Cited by 1 publication

References 102 publications

Ectopic expression of a truncated NLR gene from wild Arachis enhances resistance to Fusarium oxysporum

Ectopic expression of a truncated NLR gene from wild Arachis enhances resistance to Fusarium oxysporum

Contact Info

Product

Resources

About