A Wnt/β-catenin negative feedback loop represses TLR-triggered inflammatory responses in alveolar epithelial cells

Li, Yong; Shi, Juan; Yang, Jiali; Ma, Yan; Cheng, Long; Zeng, Jin; Hao, Xiujing; Ma, Chi; Wang, Yujiong; Liu, Xiaoming

doi:10.1016/j.molimm.2014.02.002

Cited by 41 publications

(36 citation statements)

References 36 publications

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“…The levels of protein expression were semiquantified by optical densitometry using ImageJ Software version 1.46 (https://rsb.info.nih.gov/ij/). The ratio of the net intensity of each sample divided by the β -actin internal control was calculated as densitometric arbitrary units (AU) which was served as an index of relative expression of a protein of interest [28, 29]. …”

Section: Methodsmentioning

confidence: 99%

Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5a/PKC Signaling Pathway

Yang

Zhang

et al. 2016

Stem Cells International

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The development of chemoresistance to cisplatin regimens causes a poor prognosis in patients with advanced NSCLC. The role of noncanonical Wnt signaling in the regulation of properties of lung cancer stem cells and chemoresistance was interrogated, by accessing capacities of cell proliferation, migration, invasion, and clonogenicity as well as the apoptosis in A549 cell lines and cisplatin-resistant A549 cells treated with Wnt5a conditional medium or protein kinase C (PKC) inhibitor GF109203X. Results showed that the noncanonical Wnt signaling ligand, Wnt5a, could promote the proliferation, migration, invasion, and colony formation in A549 lung adenocarcinoma cells and cisplatin-resistant A549/DDP cells and increase the fraction of ALDH-positive cell in A549/DDP cells. An exposure of cells to Wnt5a led to a significant reduction of A549/DDP cell apoptosis but not A549 cells. An addition of GF109203X could both strikingly increase the baseline apoptosis and resensitize the Wnt5a-inhibited cell apoptosis. Interestingly, an inhibition of Wnt/PKC signaling pathway could reduce properties of lung cancer stem cells, promote cell apoptosis, and resensitize cisplatin-resistant cells to cisplatin via a caspase/AIF-dependent pathway. These data thus suggested that the Wnt5a could promote lung cancer cell mobility and cisplatin-resistance through a Wnt/PKC signaling pathway and a blockage of this signaling may be an alternative therapeutic strategy for NSCLC patients with resistance to chemotherapies.

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Section: Methodsmentioning

confidence: 99%

Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5a/PKC Signaling Pathway

Yang

Zhang

et al. 2016

Stem Cells International

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“…On the other hand, other studies have shown that enhancement of ␤-catenin activity could ameliorate TLR ligand-induced pro-inflammatory responses [45]. It is suggested that in anti-inflammatory state, ␤-catenin physically binds to NF-B and prevents its activation, thereby negatively regulate inflammation [46].…”

Section: ˇ-Catenin Signalingmentioning

confidence: 99%

Glycogen synthase kinase-3 beta (GSK-3β) signaling: Implications for Parkinson's disease

Golpich

Amini

Hemmati

et al. 2015

Pharmacological Research

234

175

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“…Key genes in the WNT signaling pathway were selected based on their biological functions as core components of this pathway, including CTNNB1 , WIF1 , DKK1 , SFRP1 , WNT1 , WNT3A and WNT5A 9101112131415. We selected candidate SNPs for this study through a thorough search of the International HapMap project, dbSNP database, 1000 Genomes database.…”

Section: Methodsmentioning

confidence: 99%

“…Antagonists, including the secreted frizzled-related protein ( SFRP ) family, Wnt inhibitory protein one ( WIF1 ) and Dickkopf ( DKK ), inhibit WNT pathway signaling by binding to Wnt or Wnt receptors and negatively regulating target gene expression9. Recent evidence has indicated that the WNT pathway has an etiological role in TB disease1112131415. Wu et al .…”

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confidence: 99%

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Pathway Analyses Identify Novel Variants in the WNT Signaling Pathway Associated with Tuberculosis in Chinese Population

Zhou

Chen

et al. 2016

Sci Rep

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Tuberculosis remains a global public health problem, and its immunopathogenesis is still poorly understood. In this study, 25 single nucleotide polymorphisms (SNPs) in the WNT pathway were evaluated in relation to tuberculosis risk in a Chinese Han discovery set, and 6 candidate susceptible SNPs were further validated in a Chinese Tibetan cohort. Luciferase reporter assay, RT-qPCR and Western blot were used to assess the functionality of the important WNT polymorphisms. Five polymorphisms were associated with tuberculosis susceptibility after Bonferroni correction: SFRP1 rs4736958, CTNNB1 rs9859392, rs9870255 and rs3864004 showed decreased tuberculosis risk; SFRP1 rs7832767 was related to an increased risk (OR = 1.81, 95% CI = 1.30–2.52, p = 0.010). Patients with TT genotype of rs4736958 and rs7832767 correlated with higher CRP concentrations (p = 0.003, <0.001, respectively). Functional assays revealed that mutant alleles of rs9859392 (G), rs9870255 (C) and rs3864004 (A) were associated with significantly decreased transcriptional activity, lower CTNNB1 mRNA expression and p-β-catenin level, which were consistent with their effects of decreasing TB risk. Our results provide evidences that WNT pathway polymorphisms influence tuberculosis susceptibility and host immune response to Mycobacterium tuberculosis, suggesting that these variations may serve as novel markers for identifying the risk of developing tuberculosis.

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A Wnt/β-catenin negative feedback loop represses TLR-triggered inflammatory responses in alveolar epithelial cells

Cited by 41 publications

References 36 publications

Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5a/PKC Signaling Pathway

Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5a/PKC Signaling Pathway

Glycogen synthase kinase-3 beta (GSK-3β) signaling: Implications for Parkinson's disease

Pathway Analyses Identify Novel Variants in the WNT Signaling Pathway Associated with Tuberculosis in Chinese Population

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