A WRKY Transcription Factor Regulates Fe Translocation under Fe Deficiency

Yan, Jun; Li, Chun Xiao; Sun, Li; Ren, Jiaqi; Li, Gui Xin; Ding, Ziqian; Zheng, Shao Jian

doi:10.1104/pp.16.00252

Cited by 81 publications

(32 citation statements)

References 54 publications

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“…In addition to these transcriptional networks, WRKY46, a WRKY transcription factor family member, is involved in Fe distribution. WRKY46 regulates Fe translocation from root to shoot and appears to act independently of FIT (Yan et al, 2016). Hence, clearly, a complicated transcriptional regulatory cascade is involved in fine-tuning the plant response to Fe starvation.…”

Section: Discussionmentioning

confidence: 99%

S‐Nitrosoglutathione works downstream of nitric oxide to mediate iron‐deficiency signaling in Arabidopsis

Kailasam

Wang

et al. 2018

The Plant Journal

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Iron (Fe) is essential for plant growth and development. Knowledge of Fe signaling, from the beginning of perception to activation of the uptake process, is critical for crop improvement. Here, by using chemical screening, we identified a small molecule 3-amino-N-(3-methylphenyl)thieno[2,3-b]pyridine-2-carboxamide named R7 ('R' denoting repressor of IRON-REGULATED TRANSPORTER 1), that modulates Fe homeostasis of Arabidopsis. R7 treatment led to reduced Fe levels in plants, thus causing severe chlorosis under Fe deficiency. Expression analysis of central transcription factors, FER-LIKE IRON DEFICIENCY INDUCED TRANSCRIPTION FACTOR (FIT) and subgroup Ib basic helix-loop-helix (Ib bHLH) genes bHLH38/39/100/101, revealed that R7 targets the FIT-dependent transcriptional pathway. Exogenously supplying S-nitrosoglutathione (GSNO), but not other nitric oxide (NO) donors sodium nitroprusside (SNP) and S-nitroso-N-acetyl-dl-penicillamine (SANP), alleviated the inhibitory effects of R7 on Fe homeostasis. R7 did not inhibit cellular levels of NO or glutathione but decreased GSNO level in roots. We demonstrate that NO is involved in regulating not only the FIT transcriptional network but also the Ib bHLH networks. In addition, GSNO, from S-nitrosylation of glutathione, specifically mediates the Fe-starvation signal to FIT, which is distinct from the NO to Ib bHLH signal. Our work dissects the molecular connection between NO and the Fe-starvation response. We present a new signaling route whereby GSNO acts downstream of NO to trigger the Fe-deficiency response in Arabidopsis.

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Section: Discussionmentioning

confidence: 99%

S‐Nitrosoglutathione works downstream of nitric oxide to mediate iron‐deficiency signaling in Arabidopsis

Kailasam

Wang

et al. 2018

The Plant Journal

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“…Soybeans utilize the strategy I response, which involves increased ferric reductase activity at the root surface to convert ferric (Fe 3+ ) to ferrous (Fe 2+ ) iron, which can then be transported into the plant root by specific Fe transporters. In Arabidopsis thaliana, genes regulating these responses have largely been identified through reverse genetic approaches (Henriques et al 2002;Rogers and Guerinot 2002;Vert et al 2002;Bauer et al 2007;Long et al 2010;Yan et al 2016). In contrast, soybean studies over the last 35 years have used traditional quantitative trait locus (QTL) mapping in field conditions, sequenced-based introgression mapping, and gene expression studies to investigate Fe deficiency tolerance (Lin et al 1997(Lin et al , 2000O'Rourke et al 2009;Severin et al 2010;Peiffer et al 2012;Atwood et al 2014;Moran Lauter et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Dynamic gene expression changes in response to micronutrient, macronutrient, and multiple stress exposures in soybean

O’Rourke

McCabe

Graham

2019

Funct Integr Genomics

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Preserving crop yield is critical for US soybean production and the global economy. Crop species have been selected for increased yield for thousands of years with individual lines selected for improved performance in unique environments, constraints not experienced by model species such as Arabidopsis. This selection likely resulted in novel stress adaptations, unique to crop species. Given that iron deficiency is a perennial problem in the soybean growing regions of the USA and phosphate deficiency looms as a limitation to global agricultural production, nutrient stress studies in crop species are critically important. In this study, we directly compared whole-genome expression responses of leaves and roots to iron (Fe) and phosphate (P i) deficiency, representing a micronutrient and macronutrient, respectively. Conducting experiments side by side, we observed soybean responds to both nutrient deficiencies within 24 h. While soybean responds largely to-Fe deficiency, it responds strongly to P i resupply. Though the timing of the responses was different, both nutrient stress signals used the same molecular pathways. Our study is the first to demonstrate the speed and diversity of the soybean stress response to multiple nutrient deficiencies. We also designed the study to examine gene expression changes in response to multiple stress events. We identified 865 and 3375 genes that either altered their direction of expression after a second stress exposure or were only differentially expressed after a second stress event. Understanding the molecular underpinnings of these responses in crop species could have major implications for improving stress tolerance and preserving yield.

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“…Notably, even though the proportion of genes with appropriate expression is higher in leaves, a large fraction (32.8%) of the iron-regulated genes in leaves of DM plants had Table I. Comparison of the expression of select iron deficiency-regulated genes (Petit et al, 2001;Stacey et al, 2002Stacey et al, , 2008Mukherjee et al, 2006;Schaaf et al, 2006;Wang et al, 2007;Klatte et al, 2009;Haydon et al, 2012;Palmer et al, 2013;Fourcroy et al, 2016;Mai et al, 2016;Sisó -Terraza et al, 2016;Yan et al, 2016;Ziegler et al, 2017) (Petit et al, 2001;Stacey et al, 2002Stacey et al, , 2008Mukherjee et al, 2006;Cohu and Pilon, 2007;Wang et al, 2007;Klatte et al, 2009) in ysl1ysl3 DM and wild-type shoots *, P , 0.05; **, P , 0.001; N.S. indicates that the difference was not significant (P .…”

Section: Iron-regulated Gene Expression In Ysl1ysl3 Dm Plantsmentioning

confidence: 99%

Iron-Nicotianamine Transporters Are Required for Proper Long Distance Iron Signaling

et al. 2017

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The mechanisms of root iron uptake and the transcriptional networks that control root-level regulation of iron uptake have been well studied, but the mechanisms by which shoots signal iron status to the roots remain opaque. Here, we characterize an Arabidopsis (Arabidopsis thaliana) double mutant, yellow stripe1-like yellow stripe3-like (ysl1ysl3), which has lost the ability to properly regulate iron deficiency-influenced gene expression in both roots and shoots. In spite of markedly low tissue levels of iron, the double mutant does not up-and down-regulate iron deficiency-induced and -repressed genes. We have used grafting experiments to show that wild-type roots grafted to ysl1ysl3 shoots do not initiate iron deficiency-induced gene expression, indicating that the ysl1ysl3 shoots fail to send an appropriate long-distance signal of shoot iron status to the roots. We present a model to explain how impaired iron localization in leaf veins results in incorrect signals of iron sufficiency being sent to roots and affecting gene expression there. Improved understanding of the mechanism of long-distance iron signaling will allow improved strategies for the engineering of staple crops to accumulate additional bioavailable iron in edible parts, thus improving the iron nutrition of the billions of people worldwide whose inadequate diet causes iron deficiency anemia.

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A WRKY Transcription Factor Regulates Fe Translocation under Fe Deficiency

Cited by 81 publications

References 54 publications

S‐Nitrosoglutathione works downstream of nitric oxide to mediate iron‐deficiency signaling in Arabidopsis

S‐Nitrosoglutathione works downstream of nitric oxide to mediate iron‐deficiency signaling in Arabidopsis

Dynamic gene expression changes in response to micronutrient, macronutrient, and multiple stress exposures in soybean

Iron-Nicotianamine Transporters Are Required for Proper Long Distance Iron Signaling

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