A Zn²⁺-specific turn-on fluorescent probe for ratiometric sensing of pyrophosphate in both water and blood serum

Abstract

A novel fluorescent sensor composed of a naphthalene functionalized tetraazamacrocycle ligand 3,6,9,15-tetraazabicyclo[9.3.1]pentadeca-1(15),11,13-triene-3-methyl naphthalene (1) and Zn(2+) has been designed and prepared, which can be utilized for selective and ratiometric sensing of pyrophosphate (PPi) over other phosphate-containing anions in aqueous solution at physiological pH. Notably, the water soluble 1 itself also exhibits a selective enhanced fluorescent response to Zn(2+), and the complex 1-Zn(2+) thus formed eventually fulfils the synergic Zn(2+) coordination-altered strategy with PPi. Furthermore, the ratiometric sensing of 1-Zn(2+) towards PPi performed well even in blood serum milieu. Finally, the sensor 1-Zn(2+) was successfully employed to monitor a real-time assay of inorganic pyrophosphatase (PPase) by means of ratiometric fluorescent measurements for the first time.

Stabilisation of Exotic Tribromide (Br3−) Anions via Supramolecular Interaction with a Tosylated Macrocyclic Pyridinophane. A Serendipitous Case

“…It was later in the spotlight for its ability to form metal complexes [6][7][8], but its wider success was achieved thanks to its utilisation as a scaffold for the preparation of new materials that have proven effective in a variety of applications. Indeed, L derivatives decorated with different hanging functionalities have been studied for several biomedical (MRI contrast agents [9][10][11][12], antiproliferative treatments [13][14][15][16], radiotherapy [17,18], enzyme mimicking [19,20] and antioxidant activity [21][22][23]), chemosensing [24][25][26][27] and catalytic [28][29][30][31][32][33] applications. Very recently, (MRI contrast agents [9][10][11][12], antiproliferative treatments [13][14][15][16], radiotherapy [17,18], enzyme mimicking [19,20] and antioxidant activity [21][22][23]), chemosensing [24][25]…”

A Highly Sensitive and Selective Fluorescent Sensor for Zinc(II) Ions Based on a 1,2,3‐Triazolyl‐Functionalized 2,2’‐Dipicolylamine (DPA)

“…As evident from Figure , the fluorescent intensity of TAZDPA/ Zn 2+ complex was highly dependent upon the pH values. In the basic medium, the fluorescent intensity of TAZDPA/ Zn 2+ complex was substancially lowered, which suggested resumption of a strong PET process from DPA to the acetophenone fluorophore due to the conceivable formation of Zn 2+ hydroxide . On the other hand, decreasing the pH towards the acid medium (pH<5) led to enhance fluorescent intensity.…”

A Simple Fluorescent Chemosensor for Detection of Zinc Ions in Some Real Samples and Intracellular Imaging in Living Cells

“…44 On the other hand, the receded decreases in fluorescence response at pH > 8.0 could be attributed to the formation of Zn 2+ ion hydroxides. 45 Hence, HEPES buffer solution (pH 7.0) was chosen as the working moiety for further studies.…”