A γ-chain complex forms a functional receptor on cloned human lymphocytes with natural killer-like activity

Moingeon, Philippe; Jitsukawa, S; Faure, Florence; Troalen, Frédéric; Triebel, Frédéric; Graziani, Marita; Forestier, F.; Bellet, Dominique; Bohuon, C; Hercend, Thierry

doi:10.1038/325723a0

Cited by 185 publications

(81 citation statements)

References 19 publications

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“…In contrast, circulating TCR g/d þ cells [36,37] and a minor subset of TCR a /b þ , CD8 þ cells which contains lymphokineactivated killer (LAK) cell precursors [38] express constitutively CD122 in the absence of CD25. This feature allows both cell types to proliferate following in vitro treatment with high-dose rIL-2.…”

Section: Discussionmentioning

confidence: 99%

Synovial fluid T cell clones from oligoarticular juvenile arthritis patients display a prevalent Th1/Th0-type pattern of cytokine secretion irrespective of immunophenotype

Gattorno¹,

Facchetti

Ghiotto

et al. 1997

Clinical and Experimental Immunology

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SUMMARYThe aim of the present study was to investigate the patterns of cytokine production by T cell clones raised from in vivo activated synovial fluid (SF) mononuclear cells (MNC) of five patients with oligoarticular juvenile arthritis (JA). Freshly isolated SF T cells were cultured in vitro with low dose recombinant IL-2 and subsequently cloned by limiting dilution. Sixty-four clones were obtained from the five patients studied. Fifty-nine clones were TCR a/b þ , either CD4 þ (n ¼ 43) or CD8 þ (n ¼ 15). The remaining five clones were TCR g/d þ , CD4 ¹ , CD8 ¹ . Clone immunophenotypes differed in the individual patients. Forty-four T cell clones were stimulated with phytohaemagglutinin (PHA) and phorbol myristate acetate (PMA) and supernatants tested for the presence of IL-2, IL-4, IL-5 and interferon-gamma (IFN-g) by ELISA or bioassays. Cytokine mRNA accumulation was tested by reverse transcriptase-polymerase chain reaction (RT-PCR). Most of 44 clones tested released large amounts of IFN-g irrespective of the immunophenotype. Of these, 27 were classified as Th1-type and 17 as Th0-type based upon the IFN-g/IL-4 ratio in culture supernatants. Finally, when 10 representative T cell clones were tested for pro-and anti-inflammatory cytokines, gene expression by RT-PCR, all of them were found to express the granulocyte-macrophage colony-stimulating factor (GM-CSF), tumour necrosis factor-alpha (TNF-a), IL-10 and transforming growth factor-beta 1 (TGF-b1) genes, and half of them IL-6 and IL-8 mRNA. In conclusion, T cell clones, that represent the progeny of in vivo activated SF T cells from oligoarticular JA patients, display heterogeneous immunophenotypes, but all share the ability to produce large amounts of IFN-g, with a predominant Th1/Th0 pattern. The expression of pro-and anti-inflammatory cytokine genes in these clones suggests that in vivo activated SF T cells modulate joint inflammation in a complex fashion.

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Section: Discussionmentioning

confidence: 99%

Synovial fluid T cell clones from oligoarticular juvenile arthritis patients display a prevalent Th1/Th0-type pattern of cytokine secretion irrespective of immunophenotype

Gattorno¹,

Facchetti

Ghiotto

et al. 1997

Clinical and Experimental Immunology

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“…It is now established that T lymphocytes of this subset express a TCR-y/S (35)(36)(37)(38) and are capable of performing nonrestricted (39,40) as well as MHC-linked specific cytolytic functions (41,42).…”

Section: Discussionmentioning

confidence: 99%

Site-restricted persistent cytomegalovirus infection after selective long-term depletion of CD4+ T lymphocytes.

Jonjić

Mutter

Weiland

et al. 1989

The Journal of Experimental Medicine

521

339

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We have established a murine model system for exploring the ability of a CD4 subset-deficient host to cope with cytomegalovirus infection, and reported three findings. First, an antiviral response of the CD8 subset of T lymphocytes could be not only initiated but also maintained for a long period of time despite a continued absence of the CD4 subset, whereas the production of antiviral antibody proved strictly dependent upon help provided by the CD4 subset. Second, no function in the defense against infection could be ascribed as yet to CD4-CD8- T lymphocytes, which were seen to accumulate to a new subset as a result of depletion of the CD4 subset. This newly arising subset did not substitute for CD4+ T lymphocytes in providing help to B lymphocytes, and was also not effective in controlling the spread of virus in host tissues. As long as a function of these cells in the generation and maintenance of a CD8 subset-mediated response is not disproved, caution is indicated with concern to an autonomy of the CD8 subset. Third, even though with delay, the CD8+ effector cells raised in the CD4 subset-deficient host were able of clear vital tissues from productive infection and to restrict asymptomatic, persistent infection to acinar glandular epithelial cells in salivary gland tissue.

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“…Several lines of evidence suggest that T cells are involved in some early, non-specific resistance mechanisms [54][55][56], particularly the localization of different subsets to different epithelia that are in contact with the external environment [24,[57][58][59][60] where they appear to function as activated cytotoxic T lymphocytes [61,62]. There is also evidence from both mice and humans [63,64] that T cells originating from the recirculating pool are reactive to self 60-70-kD heat shock proteins.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the T cell receptor Vγ region gene repertoire in bronchoalveolar lavage (BAL) and peripheral blood of atopic asthmatics and healthy subjects

Molfino

Doherty

et al. 1996

Clinical and Experimental Immunology

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SUMMARYWe analysed the T cell receptor (TCR) V°repertoire in BAL and peripheral blood (PBL) of three mild stable atopic asthmatics and two non-asthmatic controls. We used the polymerase chain reaction (PCR) to establish the expression of the four V°families, and to detect oligo or monoclonal expansion of°± T cells, we resolved the PCR products on denaturing and non-denaturing gels to find the extent of junctional diversity arising from differences in the lengths of the V(D)J junctions. We also subcloned and sequenced the PCR products to characterize fully the sequence diversity. BAL T lymphocytes from two asthmatic patients (treated with inhaled steroids) expressed only V°II and, in one of them, V°IIJ°u sage was restricted to J°P and J°P1 gene segments, contrasting with the V°J°repertoire found in his respective PBL. Analyses in denaturing and non-denaturing gels showed that the BAL V°IIJ°P and V°IIJ°P1 PCR products resolved into few bands, suggesting deletions at the junctions due to oligoclonal expansion. BAL T lymphocytes from the third asthmatic (not receiving inhaled steroids) expressed V°I, II and III, and the sequences of the in-frame TCR transcripts from this asthmatic and one healthy volunteer who expressed a similar BAL V°TCR repertoire showed clonal expansion of T cells expressing all three V°families. Our analyses showed that much of the°± T cell population found in BAL fluid of humans derives from clonally expanded T cells.

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A γ-chain complex forms a functional receptor on cloned human lymphocytes with natural killer-like activity

Cited by 185 publications

References 19 publications

Synovial fluid T cell clones from oligoarticular juvenile arthritis patients display a prevalent Th1/Th0-type pattern of cytokine secretion irrespective of immunophenotype

Synovial fluid T cell clones from oligoarticular juvenile arthritis patients display a prevalent Th1/Th0-type pattern of cytokine secretion irrespective of immunophenotype

Site-restricted persistent cytomegalovirus infection after selective long-term depletion of CD4+ T lymphocytes.

Analysis of the T cell receptor Vγ region gene repertoire in bronchoalveolar lavage (BAL) and peripheral blood of atopic asthmatics and healthy subjects

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