A Δ11 desaturase gene genealogy reveals two divergent allelic classes within the European corn borer (Ostrinia nubilalis)

Geiler, Kerry A; Harrison, Richard G.

doi:10.1186/1471-2148-10-112

Cited by 7 publications

(6 citation statements)

References 47 publications

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“…Patterns of variation and gene genealogies that we observe for OnOR4 and OnOR5 are similar to what has been seen for most other loci in ECB, including the sex‐linked loci Ldh and Kettin ( Ket ) (Dopman et al 2005; Geiler and Harrison 2010), and can be explained by recognizing that E and Z strain ECB not only share an extensive amount of ancestral polymorphism, but that patterns of variation may also be influenced by contemporary gene flow. Polymorphism data suggest that there are diverse alleles in both races, and neutrality tests even show an excess of rare variants for OnOR4 in our sample, which could be a consequence of diversifying selection acting on that locus.…”

Section: Discussionsupporting

confidence: 81%

“…The two strains of O. nubilalis offer excellent opportunities to unravel the origins of barriers to gene exchange that result from divergence in pheromone communication. Indeed, the ECB has been the focus of recent studies investigating the extent of molecular differentiation between the E and Z pheromone strains at a number of nuclear loci (Willett and Harrison 1999; Dopman et al 2005; Malausa et al 2007; Geiler and Harrison 2010). Although genetic differentiation exists, gene genealogies have revealed that the two strains share an extensive amount of polymorphism at all loci investigated thus far with the exception of pgFAR (the locus encoding a fatty acid reductase responsible for differences in pheromone production between E and Z moths) and the sex‐linked marker Tpi .…”

Section: Discussionmentioning

confidence: 99%

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GENE GENEALOGIES REVEAL DIFFERENTIATION AT SEX PHEROMONE OLFACTORY RECEPTOR LOCI IN PHEROMONE STRAINS OF THE EUROPEAN CORN BORER,OSTRINIA NUBILALIS

et al. 2011

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Males of the E and Z strains of the European corn borer Ostrinia nubilalis (Lepidoptera: Crambidae) are attracted to different blends of the same pheromone components. The difference in male behavioral response is controlled by the sex-linked locus Resp. The two types of males have identical neuroanatomy but their physiological specificity is reversed, suggesting that variation at the periphery results in behavioral change. Differences in the olfactory receptors (ORs) could explain the strain-specific antennal response and blend preference. Gene genealogies can provide insights into the processes involved in speciation and allow delineation of genome regions that contribute to reproductive barriers. We used intronic DNA sequences from five OR-encoding genes to investigate whether they exhibit fixed differences between strains and therefore might contribute to reproductive isolation. Although two genealogies revealed shared polymorphism, molecular polymorphism at three genes revealed nearly fixed differences between strains. These three OR genes map to the sex chromosome, but our data indicate that the distance between Resp and the ORs is >20 cM, making it unlikely that variation in pheromone-sensitive OR genes is directly responsible for the difference in behavioral response. However, differences in male antennal response may have their origin in the selection of strain-specific alleles.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

GENE GENEALOGIES REVEAL DIFFERENTIATION AT SEX PHEROMONE OLFACTORY RECEPTOR LOCI IN PHEROMONE STRAINS OF THE EUROPEAN CORN BORER,OSTRINIA NUBILALIS

et al. 2011

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“…However, the sequences did not show any clustering according to pheromone composition, geographical origin or life history (univoltine vs. bivoltine). In another study, ECB moths were not differentiated on the basis of Δ11 desaturase allelic diversity (Geiler & Harrison, ). The variation in intron length in the two Δ11 desaturase allelic classes did not separate ECB moths according to their pheromone race, geographical origin or voltine ecotypes.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning, mRNA expression and biological activity of the pheromone biosynthesis activating neuropeptide (PBAN) from the European corn borer, Ostrinia nubilalis

Fodor

Köblös

Kákai

et al. 2017

Insect Molecular Biology

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Pheromone biosynthesis activating neuropeptide (PBAN) is a member of the pyrokinin (FXPRLamide) insect neuropeptides. Here, we report the cloning of the gene Ostnu-PBAN from the E and Z pheromone strains of the European corn borer (ECB), Ostrinia nubilalis (Lepidoptera: Crambidae), a major pest of maize. The Ostnu-PBAN genomic sequence is > 5 kb in length and consists of six exons. The deduced amino acid sequence revealed a 200-residue precursor protein including a signal peptide, a 24-amino acid (aa) diapause hormone, a 37-aa PBAN and three other FXPRLamide neuropeptides. Our in vivo assays suggest that the 37-aa synthetic Ostnu-PBAN is hormonally active in the pheromone gland. It restores sex pheromone production to normal levels in mated females and decapitated virgins of both E and Z cultures. The results of a real-time PCR analysis indicated that Ostnu-PBAN mRNA levels reached a plateau in the brain-suboesophageal ganglion complexes 1 day after eclosion, and mating did not affect the mRNA expression. Three size classes of Ostnu-PBAN mRNA (1.9, 2.0 and 2.1 kb) were obtained, differing only in the length of the 3' untranslated region. However, there was no correlation between sequence divergence and the pheromone composition, voltinism or geographical origin (Hungary, Slovenia, Sweden, Turkey) of ECB moths.

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“…In this case slightly higher variation was found, as compared to the above common markers, however, these subtle differences on their own would not have any basis for discrimination, especially not, because it could be explained that several cryptic 11-desaturase genes are involved in the pheromone biosynthesis. Indeed, two distinct clades of ∆11-desaturase have been described for O. nubilalis which do not sort with pheromone strains (Geiler and Harrison, 2010).…”

Section: Tablementioning

confidence: 99%

Viability and pathogenicity of Rhynchosporium secalis after long-term storage

Arabi

Alek

Al-Shehadah

et al. 2019

Acta Phytopathologica et Entomologica Hungarica

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The European corn borer moth, (ECB), Ostrinia nubilalis (Lepidoptera: Crambidae, Pyraustinae) is one of the most destructive pests of maize worldwide. ECB has two pheromone-strains, separated by specific ratios of isomers of E-and Z11-tetradecenyl acetates (E11-and Z11-14Ac), but appearing morphologically identical. Accordingly, E-and Z-ECB pheromone traps are available for the respective populations for practical monitoring of the flight, however, traps for Z-strain are unreliable for practical usage in some parts of Central-Europe. E-and Z-ECB populations occur in sympatry in some areas, while in allelopatry in other areas. Determining the strains before the flight of adults, when difference in the composition of their respective pheromones is manifested, would be of practical interest for early warning. In addition to the known fatty-acyl-reductase (FAR) marker, further markers would allow more comprehensive studies. We screened the following common markers for mitochondrial and nuclear DNA regions: partial cytochrome c oxidase I (COI), cytochrome B (CytB), the second spacer of the internal transcribed spacer (ITS2), Elongation factor 1a (EF1a) and actin gene (Act). In addition, a marker of the Δ11-desaturase gene (11desat), linked to biosynthesis of female-produced sex pheromone, was also included, because we reported earlier a differential expression for this site. Three Z-ECB populations locating at distant sites within Hungary, an area where only Z-strain occurs, and an E-ECB population in Slovenia, known as the closest-occurring E-strain, were included into the study. Separate laboratory colonies were established from each population, and F 1 generations were sampled to verify the identity of pheromone strains, by analysing the composition of sex pheromone by gas chromatography linked to an electroantennographic detector (GC-EAD). Molecular studies were conducted using specimens taken from the F 2 generations. Results of genetic studies showed that there were no differences between the Z and E populations for the common markers. In contrast to this, several nucleic acid changes (11 nt in 4 positions) were found between the three Z-populations (Hungary) and the E-population (Slovenia) in the desaturase marker. Further study is required to reveal whether the differences found in this study are consistent across E-populations, thus making these markers suitable for diagnostic purposes.

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A Δ11 desaturase gene genealogy reveals two divergent allelic classes within the European corn borer (Ostrinia nubilalis)

Cited by 7 publications

References 47 publications

GENE GENEALOGIES REVEAL DIFFERENTIATION AT SEX PHEROMONE OLFACTORY RECEPTOR LOCI IN PHEROMONE STRAINS OF THE EUROPEAN CORN BORER,OSTRINIA NUBILALIS

GENE GENEALOGIES REVEAL DIFFERENTIATION AT SEX PHEROMONE OLFACTORY RECEPTOR LOCI IN PHEROMONE STRAINS OF THE EUROPEAN CORN BORER,OSTRINIA NUBILALIS

Molecular cloning, mRNA expression and biological activity of the pheromone biosynthesis activating neuropeptide (PBAN) from the European corn borer, Ostrinia nubilalis

Viability and pathogenicity of Rhynchosporium secalis after long-term storage

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