A σ54-dependent PTS permease of the mannose family is responsible for sensitivity of Listeria monocytogenes to mesentericin Y105

Dalet, Karine; Cenatiempo, Y.; Cossart, Pascale; Héchard, Yann

doi:10.1099/00221287-147-12-3263

Cited by 157 publications

(207 citation statements)

References 24 publications

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“…monocytogenes (Dalet et al, 2001). However, we subsequently observed that MptA was not expressed in the mptD deletion mutant, suggesting that the entire operon is switched off in this mutant (Gravesen et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…monocytogenes showing highlevel resistance to class IIa bacteriocins (Gravesen et al, 2002). This IIAB is part of the EII Man t permease encoded by the mptACD operon (Dalet et al, 2001). Genetic inactivation of the mptACD operon resulted in resistance to mesentericin Y105 in Ls.…”

Section: Introductionmentioning

confidence: 99%

“…Genetic inactivation of the mptACD operon resulted in resistance to mesentericin Y105 in Ls. monocytogenes and Enterococcus faecalis (Dalet et al, 2001;Héchard et al, 2001). Thus EII Man t has been proposed as a requirement for inhibition of the target cell by class IIa bacteriocins.…”

Section: Introductionmentioning

confidence: 99%

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Expression of mptC of Listeria monocytogenes induces sensitivity to class IIa bacteriocins in Lactococcus lactis

Ramnath

Arous

Gravesen³

et al. 2004

Microbiology

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Sensitivity to class IIa bacteriocins from lactic acid bacteria was recently associated with the mannose phosphotransferase system (PTS) permease, EII Man t , in Listeria monocytogenes. To assess the involvement of this protein complex in class IIa bacteriocin activity, the mptACD operon, encoding EII Man t , was heterologously expressed in an insensitive species, namely Lactococcus lactis, using the NICE double plasmid system. Upon induction of the cloned operon, the recombinant Lc. lactis became sensitive to leucocin A. Pediocin PA-1 and enterocin A also showed inhibitory activity against Lc. lactis cultures expressing mptACD. Furthermore, the role of the three genes of the mptACD operon was investigated. Derivative plasmids containing various combinations of these three genes were made from the parental mptACD plasmid by divergent PCR. The results showed that expression of mptC alone is sufficient to confer sensitivity to class IIa bacteriocins in Lc. lactis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression of mptC of Listeria monocytogenes induces sensitivity to class IIa bacteriocins in Lactococcus lactis

Ramnath

Arous

Gravesen³

et al. 2004

Microbiology

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“…The expression of this gene was highly suppressed after incubation for 240 h at 4 °C, and the treatment with nisin resulted in lower dltA expression as compared with the peptide P34. Previous studies have shown that a 1000-fold increased resistance to class IIa bacteriocins in L. monocytogenes and Enterococcus faecalis resulted from the loss of mptA expression [38,42,43]. The cell wall of L. monocytogenes is composed by a thick peptidoglycan layer containing two types of anionic polymers: teichoic acids (TA), which are covalently linked to the peptidoglycan, and lipoteichoic acids (LTAs), which are poly phosphoglycerols substituted with a Dalanyl ester or a glycosyl residue and anchored in the membrane by their glycolipid moiety [44].…”

Section: Resultsmentioning

confidence: 99%

Influence of Peptide P34 on Gene Expression of Listeria Monocytogenes and Listeria Seelegeri

Vaucher

Giongo²,

Estivales

et al. 2016

Int J Pharm Pharm Sci

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Objective: Investigate the influence of the antimicrobial peptides P34 and nisin on the expression of genes associated with components of the cell surface of Listeria monocytogenes and Listeria seeligeri.Methods: Antimicrobial activity was determined by addition of peptide P34 and nisin (12.5 µg/ml) onto Brain Heart Infusion agar (BHI) plates previously inoculated with indicator strains (L. monocytogenes ATCC 7644 or L. seeligeri AC 82/4) after incubation for 24 h at 37 °C or 240 h at 4 °C. Ribonucleic acid (RNA) was directly extracted from bacterial colonies at the border of the inhibition zones, and the expression levels of genes Dalanine-D-alanyl carrier protein ligase (dltA), putative phospholipid lysinylation (Imo 1695) and EIIAB Man Results: A non-significant increase in the levels of transcription of genes dltA, Imo1695 and mptA was observed for L. monocytogenes treated with peptide P34 or nisin. Both peptides caused a similar decrease in dltA gene expression in L. seeligeri. The expression of gene Imo1695 significantly decreased (about 2000-fold) after treatment with the peptide P34 at 37 °C, while at 4 °C a reduction of 12-fold and 5-fold was detected for P34 and nisin, respectively. A significant decrease in mptA gene expression was observed by exposition to peptide P34 (31.872-fold) and nisin (16.047-fold) for 24 h at 37 °C.of mannose-specific PTS (mptA) were determined using real-time PCR. Conclusion:The results suggest that both peptide P34 and nisin influence the expression of genes related with the cell-surface/cell-membrane structure of L. seeligeri and in lesser extent L. monocytogenes.

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“…By inactivating this operon by directed mutagenesis in L. monocytogenes and E. faecalis, the mutant strains displayed high resistance to the class IIa bacteriocin mesentericin Y105 (Dalet et al 2001;Hechard et al 2001). Heterologous expression of mptACD operon of L. monocytogenes in an insensitive Lc.…”

Section: Class Iia Bacteriocinsmentioning

confidence: 99%

Genetic characterisation and heterologous expression of leucocin C, a class IIa bacteriocin from Leuconostoc carnosum 4010

Wan

Saris

et al. 2012

Appl Microbiol Biotechnol

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A σ54-dependent PTS permease of the mannose family is responsible for sensitivity of Listeria monocytogenes to mesentericin Y105

Abstract: Sensitivity of

Cited by 157 publications

References 24 publications

Expression of mptC of Listeria monocytogenes induces sensitivity to class IIa bacteriocins in Lactococcus lactis

Expression of mptC of Listeria monocytogenes induces sensitivity to class IIa bacteriocins in Lactococcus lactis

Influence of Peptide P34 on Gene Expression of Listeria Monocytogenes and Listeria Seelegeri

Genetic characterisation and heterologous expression of leucocin C, a class IIa bacteriocin from Leuconostoc carnosum 4010

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