2002
DOI: 10.1254/jjp.89.366
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A1 and A2 Adenosine Receptor Activation Inversely Modulates Potassium Currents and Membrane Potential in DDT1 MF-2 Smooth Muscle Cells

Abstract: ABSTRACT-Adenosine receptors are widely distributed in mammalian tissues and have been possibly involved through transmembrane potential changes in cell function regulation. The effect of A1 and A2A adenosine receptor ligands on transmembrane potential measured with flow cytometry and potassium conductance measured by the patch-clamp technique was investigated in DDT1 MF-2 smooth muscle cells. The A1 adenosine-receptor agonist CPA (50 nM) and the A2A adenosine-receptor agonist CGS 21680 (50 nM) elicited a rapi… Show more

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Cited by 8 publications
(2 citation statements)
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“…Thus, it is possible that 10 µM CPA is interacting with A 2 receptors expressed by the mouse microvasculature; however, currently there are no data documenting the expression profiles for adenosine receptors in mouse kidney. It is also possible that the dilation reflects activation of other vasodilatory mechanisms such as stimulation of K channels (53)(54)(55).…”
Section: Discussionmentioning
confidence: 99%
“…Thus, it is possible that 10 µM CPA is interacting with A 2 receptors expressed by the mouse microvasculature; however, currently there are no data documenting the expression profiles for adenosine receptors in mouse kidney. It is also possible that the dilation reflects activation of other vasodilatory mechanisms such as stimulation of K channels (53)(54)(55).…”
Section: Discussionmentioning
confidence: 99%
“…Activation of the A 1 AR has been shown to hyperpolarize cells (50). When membrane potential changes were measured in the same cells using a fluorescence method, CPA caused hyperpolarization in CHO cells expressing the wild-type A 1 AR with an EC 50 value of 10.1 ± 2.6 nM ( Figure 5).…”
Section: Functional Effects Of Nucleosides At Wild-type and Mutant Rementioning
confidence: 98%