A2144G Is the Main Mutation in the 23S rRNA Gene of Helicobacter pylori Associated with Clarithromycin Resistance

Momynaliev, K. Т.; Selezneva, O. V.; Козлова, А. А.; Vereshchagin, V. A.; Ilina, Elena; Govorun, Vadim M.

doi:10.1007/s11177-005-0205-7

Cited by 3 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mutation A2144G was found in an H. pylori isolate from Túquerres, with a frequency of 0.25 (1/4), which corroborates findings which suggest that the mutation is clearly associated with in vitro clarithromycin resistance[ 18 - 20 ]. It was found that the frequency in the sampled population in this study, is in line with the frequencies reported in other regions, 0.01 (1/73)[ 21 ] and 0.81(9/11)[ 20 - 23 ].…”

Section: Discussionsupporting

confidence: 74%

“…The mutations associated with clarithromycin resistance in the H. pylori isolates described in this study (A2144G, C2196T, and T2183C), are located in 23S rRNA gene V domain, as reported in the current literature[ 3 ]. Inhibition of the action of the macrolide may be due to spatial alterations in the V domain of 23S rRNA gene, which inhibit the target, as seen in transversion mutations A2143G, A2142G, A2142C[ 3 ], A2144G[ 18 , 19 , 22 ], where a nitrogenous base with two H groups (Adenine) is changed for another with three H groups (Guanine and Cytosine), with the inherent spatial alteration of the molecular structure, a phenomenon similar in transitions C2196T and T2183C[ 17 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Punctual mutations in23S rRNAgene of clarithromycin-resistantHelicobacter pyloriin Colombian populations

Matta

Ríos

Pazos

2018

WJG

View full text Add to dashboard Cite

AIMTo characterize punctual mutations in 23S rRNA gene of clarithromycin-resistant Helicobacter pylori (H. pylori) and determine their association with therapeutic failure.METHODSPCR products of 23S rRNA gene V domain of 74 H. pylori isolates; 34 resistant to clarithromycin (29 from a low-risk gastric cancer (GC) population: Tumaco-Colombia, and 5 from a high-risk population: Tuquerres-Colombia) and 40 from a susceptible population (28 from Tumaco and 12 from Túquerres) were sequenced using capillary electrophoresis. The concordance between mutations of V domain 23S rRNA gene of H. pylori and therapeutic failure was determined using the Kappa coefficient and McNemar’s test was performed to determine the relationship between H. pylori mutations and clarithromycin resistance.RESULTS23S rRNA gene from H. pylori was amplified in 56/74 isolates, of which 25 were resistant to clarithromycin (20 from Tumaco and 5 from Túquerres, respectively). In 17 resistant isolates (13 from Tumaco and 4 from Túquerres) the following mutations were found: A1593T1, A1653G2, C1770T, C1954T1, and G1827C in isolates from Tumaco, and A2144G from Túquerres. The mutations T2183C, A2144G and C2196T in H. pylori isolates resistant to clarithromycin from Colombia are reported for the first time. No association between the H. pylori mutations and in vitro clarithromycin resistance was found. However, therapeutic failure of eradication treatment was associated with mutations of 23S rRNA gene in clarithromycin-resistant H. pylori (κ = 0.71).CONCLUSIONThe therapeutic failure of eradication treatment in the two populations from Colombia was associated with mutations of the 23S rRNA gene in clarithromycin-resistant H. pylori.

show abstract

Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Punctual mutations in23S rRNAgene of clarithromycin-resistantHelicobacter pyloriin Colombian populations

Matta

Ríos

Pazos

2018

WJG

View full text Add to dashboard Cite

show abstract

“…International and national studies of point mutations related to clarithromycin resistance have been published. Momynaliev et al (30) reported that in 10 analyzed clarithromycin-resistant clinical isolates of H. pylori obtained in Russia, resistance was found to be mediated solely by the A2144G mutation in the 23S rRNA gene. The prevalence of each point mutation varies according to geographical area.…”

Section: Ethical Considerationsmentioning

confidence: 99%

Antimicrobial resistance of Helicobacter pylori strains to five antibiotics, including levofloxacin, in Northwestern Turkey

Caliskan

Tokman

Erzın

et al. 2015

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: Antibiotic resistance is the main factor that affects the effi cacy of current therapeutic regimens against Helicobacter pylori. This study aimed to determine the rates of resistance to effi cacy clarithromycin, amoxicillin, tetracycline, levofl oxacin and metronidazole among H. pylori strains isolated from Turkish patients with dyspepsia. Methods: H. pylori was cultured from corpus and antrum biopsies that were collected from patients with dyspeptic symptoms, and the antimicrobial susceptibility of H. pylori was determined using the E-test (clarithromycin, amoxicillin, tetracycline, metronidazole and levofl oxacin) according to the EUCAST breakpoints. Point mutations in the 23S rRNA gene of clarithromycin-resistant strains were investigated using realtime PCR. Results: A total of 98 H. pylori strains were isolated, all of which were susceptible to amoxicillin and tetracycline. Of these strains, 36.7% (36/98) were resistant to clarithromycin, 35.5% (34/98) were resistant to metronidazole, and 29.5% (29/98) were resistant to levofl oxacin. Multiple resistance was detected in 19.3% of the isolates. The A2143G and A2144G point mutations in the 23S rRNA-encoding gene were found in all 36 (100%) of the clarithromycin-resistant strains. Additionally, the levofl oxacin MIC values increased to 32 mg/L in our H. pylori strains. Finally, among the clarithromycin-resistant strains, 27.2% were resistant to levofl oxacin, and 45.4% were resistant to metronidazole. Conclusions: We conclude that treatment failure after clarithromycin-or levofl oxacinbased triple therapy is not surprising and that metronidazole is not a reliable agent for the eradication of H. pylori infection in Turkey.

show abstract

“…Other minisequencing protocols have been established to detect fluoroquinolone resistance related SNPs in N. gonorrhoeae [ 171 ], clarithromycin resistance in Helicobacter pylori [ 172 ], and rifampin and isoniazid-resistance in M. tuberculosis [ 173 ].…”

Section: Mass Spectrometric Approachesmentioning

confidence: 99%

Emerging Rapid Resistance Testing Methods for Clinical Microbiology Laboratories and Their Potential Impact on Patient Management

Frickmann

Masanta

Zautner

2014

BioMed Research International

View full text Add to dashboard Cite

Atypical and multidrug resistance, especially ESBL and carbapenemase expressing Enterobacteriaceae, is globally spreading. Therefore, it becomes increasingly difficult to achieve therapeutic success by calculated antibiotic therapy. Consequently, rapid antibiotic resistance testing is essential. Various molecular and mass spectrometry-based approaches have been introduced in diagnostic microbiology to speed up the providing of reliable resistance data. PCR- and sequencing-based approaches are the most expensive but the most frequently applied modes of testing, suitable for the detection of resistance genes even from primary material. Next generation sequencing, based either on assessment of allelic single nucleotide polymorphisms or on the detection of nonubiquitous resistance mechanisms might allow for sequence-based bacterial resistance testing comparable to viral resistance testing on the long term. Fluorescence in situ hybridization (FISH), based on specific binding of fluorescence-labeled oligonucleotide probes, provides a less expensive molecular bridging technique. It is particularly useful for detection of resistance mechanisms based on mutations in ribosomal RNA. Approaches based on MALDI-TOF-MS, alone or in combination with molecular techniques, like PCR/electrospray ionization MS or minisequencing provide the fastest resistance results from pure colonies or even primary samples with a growing number of protocols. This review details the various approaches of rapid resistance testing, their pros and cons, and their potential use for the diagnostic laboratory.

show abstract

A2144G Is the Main Mutation in the 23S rRNA Gene of Helicobacter pylori Associated with Clarithromycin Resistance

Cited by 3 publications

References 31 publications

Punctual mutations in23S rRNAgene of clarithromycin-resistantHelicobacter pyloriin Colombian populations

Punctual mutations in23S rRNAgene of clarithromycin-resistantHelicobacter pyloriin Colombian populations

Antimicrobial resistance of Helicobacter pylori strains to five antibiotics, including levofloxacin, in Northwestern Turkey

Emerging Rapid Resistance Testing Methods for Clinical Microbiology Laboratories and Their Potential Impact on Patient Management

Contact Info

Product

Resources

About