A23187 and red cells: Changes in deformability, K+, Mg2+, Ca2+ and ATP

Kirkpatrick, Francis H.; Hillman, Diane G.; Celle, P. L. La

doi:10.1007/bf01944610

Cited by 98 publications

(18 citation statements)

References 12 publications

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“…Blum and Hoffman (1972) and Passow ( 1963) have reported that increased Ca~ + affects only K + and not Na + permeability, but Romero andWhittam (1971), Porzig (1973), Dunn (1974) and Kirkpatrick, Hillman and LaCelle (1975) have also observed that increased Ca 2+ induces changes in Na +. This apparent conflict probably reflects differences in [Ca2+]i.…”

Section: Discussionmentioning

confidence: 93%

Effect of ionophore A23187 upon membrane function and ion movement in human and toad erythrocytes

1977

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Addition of 0.1-0.3 micronM A23187, a divalent cation ionophore, to human erythrocytes suspended in a 1.0 mM 45Ca2+ -containing buffer results in a small (approximately two fold) increase in [Ca2+]i, a significant decrease in osmotic fragility, and a decrease in intracellular K+ (100 mmoles/liter of cells to 70 mmoles/liter cells) without significant alteration of intracellular [Na+]. This decrease in [K+]i is associated with a significant decrease in packed cell volume and correlates directly with the observed alteration is osmotic fragility. Increasing extracellular K+ to 125 mM prevents the A23187-induced changes in osmotic fragility, K+ content and cell volume, but does not prevent the ionophore-induced uptake of 45Ca2+. Addition of 0.1-0.3 micronM A23187 to toad erythrocytes leads to an increase in 45Ca2+ uptake comparable to that observed in human erythrocytes, but does not alter osmotic fragility, cell volume or K+ content. Higher concentrations of ionophore (3.0-10.0 micronM) cause a 30- to 50-fold increase in 45Ca2+ uptake and concomitant change in K+ content, cell volume and osmotic fragility. These changes in cell properties can be prevented by increasing extracellular [K+] to 90 mM. The difference in sensitivity of the two cell types to A23187 is attributed to the presence of additional intracellular calcium pools within toad erythrocytes that prevent an increase in cytoplasmic Ca2+ until Ca2+ uptake is increased substantially at the higher concentrations of A23187.

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Section: Discussionmentioning

confidence: 93%

Effect of ionophore A23187 upon membrane function and ion movement in human and toad erythrocytes

1977

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“…Thus, a rise in internal Ca 2+ leads to changes in cell shape and volume, increased cellular rigidity and hemolysis (Weed et al, 1969;Palek et al, 1974;Kirkpatrick et al, 1975). Such alterations seem to arise from Ca 2+ interactions with various molecular targets.…”

Section: Intracellular Calcium Contentmentioning

confidence: 99%

Erythrocyte: Programmed Cell Death

Vittori¹,

Vota²,

Nesse³

2012

Anemia

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“…Cation transport (BLUM et al 1972;ENGELMANN et al 1987), aggregation (FRIEDRICHS et al 1989) and many membrane bound enzymes are also regulated by CaZ+. An increase of intracellular CaZ+ concentration leads for example to changes of cell shape, loss of membrane deformability and reduced live span (EATON et al 1973;KIRKPATRICK et al 1975;LA CELLE et al 1973).…”

Section: Introductionmentioning

confidence: 99%

Fluorescence measurement of free calcium in erythrocytes of rats using the ca²⁺ indicator Fura‐2

Loipführer

Reichlmayr‐Lais

Kirchgeßner

1992

Animal Physiology Nutrition

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Zusammenfassung Fluoreszenz‐Messungen zur Bestimmung des freien Calciums in Ratten‐Erythrozyten mit Hilfe des Fluoreszenz‐Indikators Fura‐2 Die Konzentration des freien Calciums wurde in intakten Ratten‐Erythrozyten mit Hilfe des Fluoreszenz‐Indikators Fura‐2 bestimmt. Zu diesem Zweck wurde die Erythrozyten‐Suspension mit dem Acetoxy‐methylester des Fura‐2 (Fura‐2‐AM) während 90 Minuten inkubiert. Als ideale Inkubationsbedingungen wurde eine Indikatorkonzentration von 500 nM und ein Hämatokrit von 0,1% eruiert. Um Verfälschungen der Meßdaten durch Blutplättchen zu vermeiden, ist es unbedingt notwendig, die Erythrozytenfraktion zu reinigen. Als mittlerer Wert für die freie Konzentration an Calcium in Erythrozyten wurde 54 ± 2 nM gemessen. Dieser Wert ist reproduzierbar auch nach Lagerung des Gesamtblutes bei 4°C bis zu 24 Stunden.

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A23187 and red cells: Changes in deformability, K+, Mg2+, Ca2+ and ATP

Cited by 98 publications

References 12 publications

Effect of ionophore A23187 upon membrane function and ion movement in human and toad erythrocytes

Effect of ionophore A23187 upon membrane function and ion movement in human and toad erythrocytes

Erythrocyte: Programmed Cell Death

Fluorescence measurement of free calcium in erythrocytes of rats using the ca²⁺ indicator Fura‐2

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A23187 and red cells: Changes in deformability, K+, Mg2+, Ca2+ and ATP

Cited by 98 publications

References 12 publications

Effect of ionophore A23187 upon membrane function and ion movement in human and toad erythrocytes

Effect of ionophore A23187 upon membrane function and ion movement in human and toad erythrocytes

Erythrocyte: Programmed Cell Death

Fluorescence measurement of free calcium in erythrocytes of rats using the ca2+ indicator Fura‐2

Contact Info

Product

Resources

About

Fluorescence measurement of free calcium in erythrocytes of rats using the ca²⁺ indicator Fura‐2