A23187-induced pulmonary gas trapping and inflammation in the guinea pig

Abstract

A brief A23187 aerosol exposure produced prolonged airway obstruction with granulocyte accumulation in conscious guinea pigs. Aminophylline, atropine, pyrilamine, salbutamol, SC-41930 (a leukotriene B4 antagonist) and WEB 2086 (a platelet activating factor antagonist) were administered intravenously (i.v.) to evaluate their ability to prevent these changes. Inhaled salbutamol was also assessed. Aminophylline, atropine, and salbutamol (i.v. and aerosol) inhibited A23187-induced gas trapping (p less than 0.01). However, pyrilamine, SC-41930 and WEB 2086 did not influence this airway obstructive effect. Only atropine, inhaled salbutamol and SC-41930 inhibited the cell influx (p less than 0.01), while pyrilamine potentiated the inflammation (p less than 0.05). We conclude that A23187 produces a sustained bronchospasm and an intense granulocyte accumulation. The treatment agents tested differ considerably in their ability to alter A23187-induced airway obstruction and inflammation.

Effects of the calcium ionophore A23187 on airway responsiveness to histamine and substance P in guinea pigs

“…This corroborated the results of Stengel et al [1,9]. In addition, we showed a significant correlation between the degree of AHR to substance P and the number of epithelial cells in the BALF.…”

“…The divalent cationic ionophore A23187 is a nonimmunogenic substance that induces bronchoconstriction and airway changes characterized by mild epithelial injury, peribronchial accumulation of granulocytes, and edema of the airways [1]. Such findings have been observed in patients with bronchial asthma, and may contribute to their characteristic airway hyperresponsiveness (AHR) [2].…”

Sulfuric acid induces airway hyperresponsiveness to substance P in the guinea pig

“…Whereas the degree of airway hyperresponsiveness to substance P was similar for sulfuric acid-and A23187-exposed guinea pigs, the pattern of lung inflammation differed. Airway epithelial damage and peribronchiolar inflammation may have con tributed to the hyperresponsiveness we observe( with A23187 [6,8]. However, the minimal sulfuri~ acid-related histological changes suggests a dis C131 sociation between airway inflammation and increased responsiveness.…”

Anti-asthma effect of an antiviral drug, acyclovir: a clinical case and experimental study

“…Acyclovir TBX Aminophyliine and his colleagues [2][3][4][5]. They have evaluated the bronchoconstriction after A23187 inhalatioti by post-mortem pulmonary gas trapping.…”

“…Stengel et al have shown that inhalation of calcium ionophore A23I87 results In airway obstruction with infiltration of peribronchial inflammatory cells, mainly eosinophils. in guinea pigs [2][3][4][5][6], Although an actively sensitized animal model frequently requires an antihistaminic drug to prevent systemic anaphylaxis, the A23187 inhalation requires no antihistaminic dmg to prevent systemic anaphylaxis. So an A23I87 inhalation asthmatic mode!…”